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Team:NJAU China/Safety
From 2014.igem.org
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<p>As outstanding progress has been made after all these years in the field of synthetic biology, an increasing number of scientists have been devoted to the creation of artificial bio-system even a life. While just like every coin owning two sides, this novel technique could also bring calamity if it were mis-used or beyond control. As iGEMers, it is our duty to reflect more about the safety to ensure the potential negative influence would hardly hinder human’s daily life and jeopardize environment.</p> | <p>As outstanding progress has been made after all these years in the field of synthetic biology, an increasing number of scientists have been devoted to the creation of artificial bio-system even a life. While just like every coin owning two sides, this novel technique could also bring calamity if it were mis-used or beyond control. As iGEMers, it is our duty to reflect more about the safety to ensure the potential negative influence would hardly hinder human’s daily life and jeopardize environment.</p> | ||
<h1>Potential Risks</h1> | <h1>Potential Risks</h1> | ||
| - | <p> | + | <p>To humans, as one species of symbiotic bacteria in human’s gut, if engineered <i>Escherichia Coli</i> enter the gut of the mankind due to the incorrect operation, the disorder of normal metabolism may get induced.</p> |
| - | < | + | <p>To the environment, the culture and some kind of reagents may cause contamination to water and soil. In addition, inserted genes may get leaked and insert into other creatures’ genome, which may cause unknown risks.</p> |
| - | < | + | <p>Our project contains a facilitating module in our project which makes use of CRISPRi system to repress certain gene. If this novel repression tools were mis-used by either evil individuals or criminal groups and were targeted on house-keeping genes of human, severe harm would been done to human’s prosperity. |
| - | <h1> | + | <h1>Safe Operations</h1> |
<p>Cooperation is an important element during the project. With the help of WHU-China for offering us Streptococcus thermophiles which contain gshF and dCas9 protein, we cast our idea more efficient.</p> | <p>Cooperation is an important element during the project. With the help of WHU-China for offering us Streptococcus thermophiles which contain gshF and dCas9 protein, we cast our idea more efficient.</p> | ||
<p> Besides the help of WHU-China, we also have a tight connection with CAU_China.They offer our team the protocol about gene synthesis, which helped us a lot about GSH part. As we have done lots of experiments about detecting florescence intensity to verify the function of our device, we give our protocol about the method to quantized the expression of GFP, our team members Xu Xuan is familiar with this experiment, he also give some suggestions their project about rpaⅠ. Via frequent communication and disscution, we both gained progress and excellent suggestions.</p> | <p> Besides the help of WHU-China, we also have a tight connection with CAU_China.They offer our team the protocol about gene synthesis, which helped us a lot about GSH part. As we have done lots of experiments about detecting florescence intensity to verify the function of our device, we give our protocol about the method to quantized the expression of GFP, our team members Xu Xuan is familiar with this experiment, he also give some suggestions their project about rpaⅠ. Via frequent communication and disscution, we both gained progress and excellent suggestions.</p> | ||
Revision as of 19:15, 17 October 2014
As outstanding progress has been made after all these years in the field of synthetic biology, an increasing number of scientists have been devoted to the creation of artificial bio-system even a life. While just like every coin owning two sides, this novel technique could also bring calamity if it were mis-used or beyond control. As iGEMers, it is our duty to reflect more about the safety to ensure the potential negative influence would hardly hinder human’s daily life and jeopardize environment.
Potential Risks
To humans, as one species of symbiotic bacteria in human’s gut, if engineered Escherichia Coli enter the gut of the mankind due to the incorrect operation, the disorder of normal metabolism may get induced.
To the environment, the culture and some kind of reagents may cause contamination to water and soil. In addition, inserted genes may get leaked and insert into other creatures’ genome, which may cause unknown risks.
Our project contains a facilitating module in our project which makes use of CRISPRi system to repress certain gene. If this novel repression tools were mis-used by either evil individuals or criminal groups and were targeted on house-keeping genes of human, severe harm would been done to human’s prosperity.
Safe Operations
Cooperation is an important element during the project. With the help of WHU-China for offering us Streptococcus thermophiles which contain gshF and dCas9 protein, we cast our idea more efficient.
Besides the help of WHU-China, we also have a tight connection with CAU_China.They offer our team the protocol about gene synthesis, which helped us a lot about GSH part. As we have done lots of experiments about detecting florescence intensity to verify the function of our device, we give our protocol about the method to quantized the expression of GFP, our team members Xu Xuan is familiar with this experiment, he also give some suggestions their project about rpaⅠ. Via frequent communication and disscution, we both gained progress and excellent suggestions.
See CAU_China Attribution Page Here: https://2014.igem.org/Team:CAU_China/Team/Attribution.html
