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Team:Groningen/Template/MODULE/Notebook/detection/week3
From 2014.igem.org
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| - | Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (<a href="http://parts.igem.org/Part: BBa_K1033225">BBa_K1033225</a>), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter | + | Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (<a href="http://parts.igem.org/Part: BBa_K1033225">BBa_K1033225</a>), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP29 (<a href="http://parts.igem.org/Part: BBa_K1033222">BBa_K1033222</a>) |
Revision as of 19:22, 17 October 2014
August 11 - August 16
Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (BBa_K1033225), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP29 (BBa_K1033222)
Both constructs were transformed to E. coli DH5α
Some blue colonies were visible on the plates, an overnight culture was prepared from these colonies
The plasmids were isolated out of the overnight cultures
The size of the constructs were digested, and checked on gel
