"
Team:Bordeaux/NotebookJune3
From 2014.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
<html> | <html> | ||
| - | <h1> | + | <h1>3<sup>rd</sup> June </h1> |
<div class="type-exp"> Molecular biology</div> | <div class="type-exp"> Molecular biology</div> | ||
| Line 10: | Line 10: | ||
<li> | <li> | ||
<div> <b>DAY TWO :</b> Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)</div> | <div> <b>DAY TWO :</b> Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)</div> | ||
| - | </li> | + | </li><br> |
<li> Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol) </li> | <li> Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol) </li> | ||
| - | </ul> | + | </ul><br> |
</body> | </body> | ||
</html> | </html> | ||
{{:Team:Bordeaux/Pied}} | {{:Team:Bordeaux/Pied}} | ||
Latest revision as of 18:40, 17 October 2014
3rd June
Molecular biology
-
DAY TWO : Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)
- Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol)
