Team:Evry/Notebook/Transposons/08.06.14

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<br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/>
<br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/>
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<img src="https://static.igem.org/mediawiki/2014/6/64/PNK2.png" width="50%"/>
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<img src="https://static.igem.org/mediawiki/2014/6/64/PNK2.png" width="50%"/><br/>

Revision as of 18:25, 17 October 2014

Picture
It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.
A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
It parallel annotation of plasmid pNK2 with Blast is realized.

Aug 08