Team:INSA-Lyon/Results
From 2014.igem.org
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- | + | <h5>Confocal Laser Scanning Microscopy Analyzes</h5> | |
- | <div align="justify"><p>For the Confocal Laser Scanning Microscopy biofilm acquisitions, all the strains were cultivated in 96-wells microplate in M63 Mannitol during 16H at 30°C (<a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">See Protocol for details</a>). See results in <b>Figure | + | <div align="justify"><p><br/>For the Confocal Laser Scanning Microscopy biofilm acquisitions, all the strains were cultivated in 96-wells microplate in M63 Mannitol during 16H at 30°C (<a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">See Protocol for details</a>). See results in <b>Figure 4</b>.</p> |
<img src="https://static.igem.org/mediawiki/2014/4/4f/Figureglobaleetoile.png" alt="les filles au labo" | <img src="https://static.igem.org/mediawiki/2014/4/4f/Figureglobaleetoile.png" alt="les filles au labo" | ||
width="900px" align="center"/> | width="900px" align="center"/> | ||
- | <p><b>Figure | + | <p><b>Figure 4: Engineered bacteria biofilm characterization and quantification using Confocal Laser Scanning Microscopy</p></b> |
<p>All the strains used are constitutively fluorescent to allow detection with confocal laser microscopy (ZEISS LSM510 META, 40X/1.3OILDIC, laser Argon 4 lines 30 W 458 nm, 477 nm, 488 nm, 514 nm, <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">See Protocol</a>). Positive control/CsgA+ (Wild-type <i>E. coli</i> curli producing strain); Negative control/CsgA- (<i>csgA</i>-knockout <i>E. coli</i> strain); BBa_CsgA (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgAHis1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgAHis2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). <b>A)</b> Biofilm sections obtained by Z-stack acquisitions. <b>B)</b> Biofilm 3D reconstruction using IMARIS® from acquisitions in A). <b>C) </b>Bio-volume quantification and maximum of thickness measurement using COMSTAT2 (ImageJ). The strain marked with a star is significantly different from all others (Tukey’s test, p<0.05).</p> | <p>All the strains used are constitutively fluorescent to allow detection with confocal laser microscopy (ZEISS LSM510 META, 40X/1.3OILDIC, laser Argon 4 lines 30 W 458 nm, 477 nm, 488 nm, 514 nm, <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">See Protocol</a>). Positive control/CsgA+ (Wild-type <i>E. coli</i> curli producing strain); Negative control/CsgA- (<i>csgA</i>-knockout <i>E. coli</i> strain); BBa_CsgA (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgAHis1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgAHis2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). <b>A)</b> Biofilm sections obtained by Z-stack acquisitions. <b>B)</b> Biofilm 3D reconstruction using IMARIS® from acquisitions in A). <b>C) </b>Bio-volume quantification and maximum of thickness measurement using COMSTAT2 (ImageJ). The strain marked with a star is significantly different from all others (Tukey’s test, p<0.05).</p> |
Revision as of 18:16, 17 October 2014