Team:Paris Saclay/Notebook/July/11

From 2014.igem.org

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(→2 - Preparation of competent cells E.coli)
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==Lab work==
==Lab work==
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====1 - Fill in the WIKI (Notebook and Protocols)====
+
===1 - Fill in the WIKI (Notebook and Protocols)===
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''Juliette & Romain''
+
''by Juliette & Romain''
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====2 - Preparation of competent cells E.coli====
+
===2 - Preparation of competent cells E.coli===
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''Maher and Sean''
+
''by Maher and Sean''
Competent strains used:  
Competent strains used:  
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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====3 - Electrophoresis====
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===3 - Electrophoresis===
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''Fabio & Marie''
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''by Fabio & Marie''
[[File:Electrophoresis_11.07.2014.jpg|400px|right]]
[[File:Electrophoresis_11.07.2014.jpg|400px|right]]

Revision as of 14:21, 15 July 2014

Contents

Friday 11th July

Lab work

1 - Fill in the WIKI (Notebook and Protocols)

by Juliette & Romain

2 - Preparation of competent cells E.coli

by Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the first day, Maher did the second one.

Protocol

3 - Electrophoresis

by Fabio & Marie

Electrophoresis 11.07.2014.jpg
  1. Cm FNR-RBS-AmilCP term (1)
  2. Cm FNR-RBS-AmilCP term (2)
  3. B0015 (1)
  4. B0015 (2)
  5. PCola-GES 10^-2 (1)
  6. PCola-GES 10^-2 (1)
  7. PCola-GES 10^-2 (2)
  8. PCola-GES 10^-2 (2)
  9. PJBG-6409 10^-2 (1)
  10. PJBG-6409 10^-2 (2)
  11. PJBG-6409 10^-2 (2)

Protocol

Back to the calendar

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