Team:Paris Saclay/Notebook/July/11

From 2014.igem.org

(Difference between revisions)
(→3 - Electrophoresis)
(→2 - Preparation of competent cells E.coli)
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*MG1655Z1
*MG1655Z1
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Sean did the day1, Maher did the day 2.
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Sean did the first day, Maher did the second one.
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]

Revision as of 09:10, 15 July 2014

Contents

Friday 11th July

Lab work

1 - Fill in the WIKI (Notebook and Protocols)

Juliette & Romain

2 - Preparation of competent cells E.coli

Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the first day, Maher did the second one.

Protocol

3 - Electrophoresis

Fabio & Marie

Electrophoresis 11.07.2014.jpg
  1. Cm FNR-RBS-AmilCP term (1)
  2. Cm FNR-RBS-AmilCP term (2)
  3. B0015 (1)
  4. B0015 (2)
  5. PCola-GES 10^-2 (1)
  6. PCola-GES 10^-2 (1)
  7. PCola-GES 10^-2 (2)
  8. PCola-GES 10^-2 (2)
  9. PJBG-6409 10^-2 (1)
  10. PJBG-6409 10^-2 (2)
  11. PJBG-6409 10^-2 (2)

Protocol

Back to the calendar

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