Team:UT-Tokyo/Counter/Project/Lab

From 2014.igem.org

(Difference between revisions)
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<div id = "Lab-1">
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  <TABLE id="Table_1" cellspacing="0" cellpadding="0" abp="28">
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    <THEAD abp="29">
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      <TR abp="30">
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        <TH nowrap="" colspan="1" abp="31">
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        <TH nowrap="" colspan="1" abp="32">
 +
        <TH abp="33">Name</TH>
 +
        <TH colspan="1" abp="34">Type
 +
        <TH colspan="1" abp="35">Description
 +
        <TH colspan="1" abp="36">Designer
 +
        <TH colspan="1" abp="37">Length
 +
        <TH abp="38">
 +
      </TR>
 +
    </THEAD>
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    <TBODY abp="39">
 +
      <!-- ROW -->
 +
    <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="40">
 +
    </FORM>
 +
    <INPUT name="Editing" type="hidden" value="Editing" abp="41">
 +
    <INPUT name="part" type="hidden" value="32349" abp="42">
 +
    <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="43">
 +
    <INPUT name="group" type="hidden" value="UT-Tokyo" abp="44">
 +
    <TR abp="45">
 +
      <TD abp="46"> </TD>
 +
      <TD abp="47">  </TD>
 +
      <TD abp="48"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461000" abp="49">BBa_K1461000</A></TD>
 +
      <TD abp="50">RBS</TD>
 +
      <TD abp="51">crRBS</TD>
 +
      <TD width="100" abp="52">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="53">52</TD>
 +
      <TD abp="54"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="55">
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      </FORM>
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      <INPUT name="Editing2" type="hidden" value="Editing" abp="56">
 +
      <INPUT name="part" type="hidden" value="32464" abp="57">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="58">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="59">
 +
    <TR abp="60">
 +
      <TD abp="61"> </TD>
 +
      <TD abp="62">  </TD>
 +
      <TD abp="63"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461001" abp="64">BBa_K1461001</A></TD>
 +
      <TD abp="65">Regulatory</TD>
 +
      <TD abp="66">ecf20 promoter</TD>
 +
      <TD width="100" abp="67">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="68">80</TD>
 +
      <TD abp="69"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="70">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="71">
 +
      <INPUT name="part" type="hidden" value="32465" abp="72">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="73">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="74">
 +
    <TR abp="75">
 +
      <TD abp="76"> </TD>
 +
      <TD abp="77">  </TD>
 +
      <TD abp="78"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461002" abp="79">BBa_K1461002</A></TD>
 +
      <TD abp="80">Regulatory</TD>
 +
      <TD abp="81">ecf11 promoter</TD>
 +
      <TD width="100" abp="82">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="83">80</TD>
 +
      <TD abp="84"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="85">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="86">
 +
      <INPUT name="part" type="hidden" value="32466" abp="87">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="88">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="89">
 +
    <TR abp="90">
 +
      <TD abp="91"> </TD>
 +
      <TD abp="92">  </TD>
 +
      <TD abp="93"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461003" abp="94">BBa_K1461003</A></TD>
 +
      <TD abp="95">RNA</TD>
 +
      <TD abp="96">taRNA</TD>
 +
      <TD width="100" abp="97">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="98">71</TD>
 +
      <TD abp="99"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="100">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="101">
 +
      <INPUT name="part" type="hidden" value="32469" abp="102">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="103">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="104">
 +
    <TR abp="105">
 +
      <TD abp="106"> </TD>
 +
      <TD abp="107">  </TD>
 +
      <TD abp="108"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461004" abp="109">BBa_K1461004</A></TD>
 +
      <TD abp="110">Coding</TD>
 +
      <TD abp="111">ecf20</TD>
 +
      <TD width="100" abp="112">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="113">582</TD>
 +
      <TD abp="114"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="115">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="116">
 +
      <INPUT name="part" type="hidden" value="32472" abp="117">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="118">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="119">
 +
    <TR abp="120">
 +
      <TD abp="121"> </TD>
 +
      <TD abp="122">  </TD>
 +
      <TD abp="123"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461005" abp="124">BBa_K1461005</A></TD>
 +
      <TD abp="125">Coding</TD>
 +
      <TD abp="126">ecf11</TD>
 +
      <TD width="100" abp="127">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="128">609</TD>
 +
      <TD abp="129"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="130">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="131">
 +
      <INPUT name="part" type="hidden" value="32473" abp="132">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="133">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="134">
 +
    <TR abp="135">
 +
      <TD abp="136"> </TD>
 +
      <TD abp="137">  </TD>
 +
      <TD abp="138"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461006" abp="139">BBa_K1461006</A></TD>
 +
      <TD abp="140">Coding</TD>
 +
      <TD abp="141">anti ecf20</TD>
 +
      <TD width="100" abp="142">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="143">432</TD>
 +
      <TD abp="144"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="145">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="146">
 +
      <INPUT name="part" type="hidden" value="32471" abp="147">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="148">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="149">
 +
    <TR abp="150">
 +
      <TD abp="151"> </TD>
 +
      <TD abp="152">  </TD>
 +
      <TD abp="153"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461007" abp="154">BBa_K1461007</A></TD>
 +
      <TD abp="155">Coding</TD>
 +
      <TD abp="156">anti ecf11</TD>
 +
      <TD width="100" abp="157">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="158">663</TD>
 +
      <TD abp="159"></TD>
 +
    </TR>
 +
    </TBODY>
 +
  </TABLE>
 +
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 +
  <BR abp="162">
 +
  <!-- Part Table -->
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    <TABLE width="865" abp="164">
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      <TBODY abp="165">
 +
        <TR abp="166">
 +
          <TD width="50%" abp="167"><strong abp="168">UT-Tokyo 2014 iGEM Team Parts Sandbox</strong>
 +
          <TD width="35%" abp="169">
 +
          <TD width="15%" align="RIGHT" abp="170"><A href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo&Editing=Editing" abp="171"><em abp="172">Edit</em></A>
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      <TR abp="175">
 +
        <TH nowrap="" colspan="1" abp="176">
 +
        <TH nowrap="" colspan="1" abp="177">
 +
        <TH abp="178">Name</TH>
 +
        <TH colspan="1" abp="179">Type
 +
        <TH colspan="1" abp="180">Description
 +
        <TH colspan="1" abp="181">Designer
 +
        <TH colspan="1" abp="182">Length
 +
        <TH abp="183">
 +
      </TR>
 +
    </THEAD>
 +
    <TBODY abp="184">
 +
      <!-- ROW -->
 +
      <!-- ROW -->
 +
      <!-- ROW -->
 +
    <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="185">
 +
    </FORM>
 +
    <INPUT name="Editing2" type="hidden" value="Editing" abp="186">
 +
    <INPUT name="part" type="hidden" value="34675" abp="187">
 +
    <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="188">
 +
    <INPUT name="group" type="hidden" value="UT-Tokyo" abp="189">
 +
    <TR abp="190">
 +
      <TD abp="191"> </TD>
 +
      <TD abp="192">  </TD>
 +
      <TD abp="193"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461008" abp="194">BBa_K1461008</A></TD>
 +
      <TD abp="195">Coding</TD>
 +
      <TD abp="196">GFP (AAV tag +)</TD>
 +
      <TD width="100" abp="197">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="198">742</TD>
 +
      <TD abp="199"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="200">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="201">
 +
      <INPUT name="part" type="hidden" value="34677" abp="202">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="203">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="204">
 +
    <TR abp="205">
 +
      <TD abp="206"> </TD>
 +
      <TD abp="207">  </TD>
 +
      <TD abp="208"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461009" abp="209">BBa_K1461009</A></TD>
 +
      <TD abp="210">Coding</TD>
 +
      <TD abp="211">GFP (AAV tag +)</TD>
 +
      <TD width="100" abp="212">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="213">742</TD>
 +
      <TD abp="214"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="215">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="216">
 +
      <INPUT name="part" type="hidden" value="32400" abp="217">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="218">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="219">
 +
    <TR abp="325">
 +
      <TD abp="326"> </TD>
 +
      <TD abp="327"> </TD>
 +
      <TD abp="328"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461200" abp="329">BBa_K1461200</A></TD>
 +
      <TD abp="330">Composite</TD>
 +
      <TD abp="331">taRNA (+d.term)</TD>
 +
      <TD width="100" abp="332">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="333">208</TD>
 +
      <TD abp="334"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="335">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="336">
 +
      <INPUT name="part" type="hidden" value="32540" abp="337">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="338">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="339">
 +
    <TR abp="340">
 +
      <TD abp="341"> </TD>
 +
      <TD abp="342"> </TD>
 +
      <TD abp="343"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461201" abp="344">BBa_K1461201</A></TD>
 +
      <TD abp="345">Composite</TD>
 +
      <TD abp="346">ecf20 (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="347">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="348">737</TD>
 +
      <TD abp="349"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="350">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="351">
 +
      <INPUT name="part" type="hidden" value="32541" abp="352">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="353">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="354">
 +
    <TR abp="355">
 +
      <TD abp="356"> </TD>
 +
      <TD abp="357"> </TD>
 +
      <TD abp="358"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461202" abp="359">BBa_K1461202</A></TD>
 +
      <TD abp="360">Composite</TD>
 +
      <TD abp="361">ecf11 (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="362">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="363">764</TD>
 +
      <TD abp="364"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="365">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="366">
 +
      <INPUT name="part" type="hidden" value="32584" abp="367">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="368">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="369">
 +
    <TR abp="370">
 +
      <TD abp="371"> </TD>
 +
      <TD abp="372">  </TD>
 +
      <TD abp="373"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461203" abp="374">BBa_K1461203</A></TD>
 +
      <TD abp="375">Composite</TD>
 +
      <TD abp="376">cr-ecf20 (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="377">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="378">777</TD>
 +
      <TD abp="379"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="380">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="381">
 +
      <INPUT name="part" type="hidden" value="32585" abp="382">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="383">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="384">
 +
    <TR abp="385">
 +
      <TD abp="386"> </TD>
 +
      <TD abp="387">  </TD>
 +
      <TD abp="388"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461204" abp="389">BBa_K1461204</A></TD>
 +
      <TD abp="390">Composite</TD>
 +
      <TD abp="391">cr-ecf11 (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="392">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="393">804</TD>
 +
      <TD abp="394"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="395">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="396">
 +
      <INPUT name="part" type="hidden" value="32586" abp="397">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="398">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="399">
 +
    <TR abp="400">
 +
      <TD abp="401"> </TD>
 +
      <TD abp="402">  </TD>
 +
      <TD abp="403"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461205" abp="404">BBa_K1461205</A></TD>
 +
      <TD abp="405">Composite</TD>
 +
      <TD abp="406">cr-GFP (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="407">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="408">915</TD>
 +
      <TD abp="409"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="410">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="411">
 +
      <INPUT name="part" type="hidden" value="32592" abp="412">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="413">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="414">
 +
    <TR abp="415">
 +
      <TD abp="416"> </TD>
 +
      <TD abp="417">  </TD>
 +
      <TD abp="418"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461206" abp="419">BBa_K1461206</A></TD>
 +
      <TD abp="420">Composite</TD>
 +
      <TD abp="421">anti ecf20 (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="422">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="423">587</TD>
 +
      <TD abp="424"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="425">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="426">
 +
      <INPUT name="part" type="hidden" value="32593" abp="427">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="428">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="429">
 +
    <TR abp="430">
 +
      <TD abp="431"> </TD>
 +
      <TD abp="432">  </TD>
 +
      <TD abp="433"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461207" abp="434">BBa_K1461207</A></TD>
 +
      <TD abp="435">Composite</TD>
 +
      <TD abp="436">anti ecf11 (+RBS, +d.term)</TD>
 +
      <TD width="100" abp="437">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="438">818</TD>
 +
      <TD abp="439"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="440">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="441">
 +
      <INPUT name="part" type="hidden" value="32596" abp="442">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="443">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="444">
 +
    <TR abp="955">
 +
      <TD abp="956"> </TD>
 +
      <TD abp="957"> </TD>
 +
      <TD abp="958"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461300" abp="959">BBa_K1461300</A></TD>
 +
      <TD abp="960">Composite</TD>
 +
      <TD abp="961">RBS-GFP-4miR-142-3p bindng sites-d.term</TD>
 +
      <TD width="100" abp="962">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="963">1007</TD>
 +
      <TD abp="964"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="965">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="966">
 +
      <INPUT name="part" type="hidden" value="32572" abp="967">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="968">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="969">
 +
    <TR abp="970">
 +
      <TD abp="971"> </TD>
 +
      <TD abp="972"> </TD>
 +
      <TD abp="973"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461301" abp="974">BBa_K1461301</A></TD>
 +
      <TD abp="975">Composite</TD>
 +
      <TD abp="976">RBS-GFP-4 miR-142-5p bindng sites-d.term</TD>
 +
      <TD width="100" abp="977">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="978">999</TD>
 +
      <TD abp="979"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="980">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="981">
 +
      <INPUT name="part" type="hidden" value="32629" abp="982">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="983">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="984">
 +
    <TR abp="985">
 +
      <TD abp="986"> </TD>
 +
      <TD abp="987"> </TD>
 +
      <TD abp="988"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461302" abp="989">BBa_K1461302</A></TD>
 +
      <TD abp="990">Composite</TD>
 +
      <TD abp="991">RBS-GFP-miR 142 3p non-binding site-d.term</TD>
 +
      <TD width="100" abp="992">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="993">1001</TD>
 +
      <TD abp="994"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="995">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="996">
 +
      <INPUT name="part" type="hidden" value="32630" abp="997">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="998">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="999">
 +
    <TR abp="1000">
 +
      <TD abp="1001"> </TD>
 +
      <TD abp="1002"> </TD>
 +
      <TD abp="1003"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461303" abp="1004">BBa_K1461303</A></TD>
 +
      <TD abp="1005">Composite</TD>
 +
      <TD abp="1006">RBS-GFP-miR 142 5p non-binding site-d.term</TD>
 +
      <TD width="100" abp="1007">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1008">1007</TD>
 +
      <TD abp="1009"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1010">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1011">
 +
      <INPUT name="part" type="hidden" value="32631" abp="1012">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1013">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1014">
 +
    <TR abp="1015">
 +
      <TD abp="1016"> </TD>
 +
      <TD abp="1017">  </TD>
 +
      <TD abp="1018"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461304" abp="1019">BBa_K1461304</A></TD>
 +
      <TD abp="1020">Composite</TD>
 +
      <TD abp="1021">RBS-GFP-2miR 142 3p bindng sites-2miR 142 5p bindng sites-d.term</TD>
 +
      <TD width="100" abp="1022">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1023">1003</TD>
 +
      <TD abp="1024"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1025">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1026">
 +
      <INPUT name="part" type="hidden" value="32632" abp="1027">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1028">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1029">
 +
    <TR abp="1030">
 +
      <TD abp="1031"> </TD>
 +
      <TD abp="1032">  </TD>
 +
      <TD abp="1033"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461305" abp="1034">BBa_K1461305</A></TD>
 +
      <TD abp="1035">Composite</TD>
 +
      <TD abp="1036">RBS-GFP-2miR 142 3p non-bindng sites-2miR 142 5p non-bindng sites-d.term</TD>
 +
      <TD width="100" abp="1037">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1038">1003</TD>
 +
      <TD abp="1039"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1040">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1041">
 +
      <INPUT name="part" type="hidden" value="32638" abp="1042">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1043">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1044">
 +
    <TR abp="445">
 +
      <TD abp="446"> </TD>
 +
      <TD abp="447"> </TD>
 +
      <TD abp="448"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461208" abp="449">BBa_K1461208</A></TD>
 +
      <TD abp="450">Generator</TD>
 +
      <TD abp="451">taRNA (arabinose-induced) (+d.term)</TD>
 +
      <TD width="100" abp="452">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="453">346</TD>
 +
      <TD abp="454"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="455">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="456">
 +
      <INPUT name="part" type="hidden" value="32602" abp="457">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="458">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="459">
 +
    <TR abp="460">
 +
      <TD abp="461"> </TD>
 +
      <TD abp="462">  </TD>
 +
      <TD abp="463"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461209" abp="464">BBa_K1461209</A></TD>
 +
      <TD abp="465">Generator</TD>
 +
      <TD abp="466">arabinose-induced ecf20 generator</TD>
 +
      <TD width="100" abp="467">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="468">875</TD>
 +
      <TD abp="469"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="470">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="471">
 +
      <INPUT name="part" type="hidden" value="32605" abp="472">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="473">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="474">
 +
    <TR abp="475">
 +
      <TD abp="476"> </TD>
 +
      <TD abp="477">  </TD>
 +
      <TD abp="478"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461210" abp="479">BBa_K1461210</A></TD>
 +
      <TD abp="480">Generator</TD>
 +
      <TD abp="481">constitutive ecf20 generator</TD>
 +
      <TD width="100" abp="482">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="483">780</TD>
 +
      <TD abp="484"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="485">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="486">
 +
      <INPUT name="part" type="hidden" value="32606" abp="487">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="488">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="489">
 +
    <TR abp="490">
 +
      <TD abp="491"> </TD>
 +
      <TD abp="492">  </TD>
 +
      <TD abp="493"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461211" abp="494">BBa_K1461211</A></TD>
 +
      <TD abp="495">Generator</TD>
 +
      <TD abp="496">arabinose-induced ecf11 generator</TD>
 +
      <TD width="100" abp="497">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="498">902</TD>
 +
      <TD abp="499"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="500">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="501">
 +
      <INPUT name="part" type="hidden" value="32608" abp="502">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="503">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="504">
 +
    <TR abp="505">
 +
      <TD abp="506"> </TD>
 +
      <TD abp="507">  </TD>
 +
      <TD abp="508"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461212" abp="509">BBa_K1461212</A></TD>
 +
      <TD abp="510">Generator</TD>
 +
      <TD abp="511">constitutive ecf11 generator</TD>
 +
      <TD width="100" abp="512">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="513">807</TD>
 +
      <TD abp="514"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="515">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="516">
 +
      <INPUT name="part" type="hidden" value="32615" abp="517">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="518">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="519">
 +
    <TR abp="520">
 +
      <TD abp="521"> </TD>
 +
      <TD abp="522">  </TD>
 +
      <TD abp="523"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461213" abp="524">BBa_K1461213</A></TD>
 +
      <TD abp="525">Generator</TD>
 +
      <TD abp="526">arabinose-induced cr-ecf20 generator</TD>
 +
      <TD width="100" abp="527">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="528">915</TD>
 +
      <TD abp="529"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="530">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="531">
 +
      <INPUT name="part" type="hidden" value="32616" abp="532">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="533">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="534">
 +
    <TR abp="535">
 +
      <TD abp="536"> </TD>
 +
      <TD abp="537">  </TD>
 +
      <TD abp="538"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461214" abp="539">BBa_K1461214</A></TD>
 +
      <TD abp="540">Generator</TD>
 +
      <TD abp="541">constitutive cr-ecf20 generator</TD>
 +
      <TD width="100" abp="542">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="543">820</TD>
 +
      <TD abp="544"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="545">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="546">
 +
      <INPUT name="part" type="hidden" value="32617" abp="547">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="548">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="549">
 +
    <TR abp="550">
 +
      <TD abp="551"> </TD>
 +
      <TD abp="552">  </TD>
 +
      <TD abp="553"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461215" abp="554">BBa_K1461215</A></TD>
 +
      <TD abp="555">Generator</TD>
 +
      <TD abp="556">arabinose-induced cr-ecf11 generator</TD>
 +
      <TD width="100" abp="557">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="558">942</TD>
 +
      <TD abp="559"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="560">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="561">
 +
      <INPUT name="part" type="hidden" value="32618" abp="562">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="563">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="564">
 +
    <TR abp="565">
 +
      <TD abp="566"> </TD>
 +
      <TD abp="567">  </TD>
 +
      <TD abp="568"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461216" abp="569">BBa_K1461216</A></TD>
 +
      <TD abp="570">Generator</TD>
 +
      <TD abp="571">constitutive cr-ecf11 generator</TD>
 +
      <TD width="100" abp="572">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="573">847</TD>
 +
      <TD abp="574"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="575">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="576">
 +
      <INPUT name="part" type="hidden" value="32619" abp="577">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="578">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="579">
 +
    <TR abp="580">
 +
      <TD abp="581"> </TD>
 +
      <TD abp="582"> </TD>
 +
      <TD abp="583"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461217" abp="584">BBa_K1461217</A></TD>
 +
      <TD abp="585">Generator</TD>
 +
      <TD abp="586">arabinose-induced cr-GFP generator</TD>
 +
      <TD width="100" abp="587">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="588">1053</TD>
 +
      <TD abp="589"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="590">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="591">
 +
      <INPUT name="part" type="hidden" value="32620" abp="592">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="593">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="594">
 +
    <TR abp="595">
 +
      <TD abp="596"> </TD>
 +
      <TD abp="597"> </TD>
 +
      <TD abp="598"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461218" abp="599">BBa_K1461218</A></TD>
 +
      <TD abp="600">Generator</TD>
 +
      <TD abp="601">constitutive cr-GFP generator</TD>
 +
      <TD width="100" abp="602">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="603">958</TD>
 +
      <TD abp="604"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="605">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="606">
 +
      <INPUT name="part" type="hidden" value="32716" abp="607">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="608">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="609">
 +
    <TR abp="610">
 +
      <TD abp="611"> </TD>
 +
      <TD abp="612"> </TD>
 +
      <TD abp="613"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461219" abp="614">BBa_K1461219</A></TD>
 +
      <TD abp="615">Generator</TD>
 +
      <TD abp="616">ecf20-induced GFP generator</TD>
 +
      <TD width="100" abp="617">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="618">963</TD>
 +
      <TD abp="619"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="620">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="621">
 +
      <INPUT name="part" type="hidden" value="32717" abp="622">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="623">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="624">
 +
    <TR abp="625">
 +
      <TD abp="626"> </TD>
 +
      <TD abp="627"> </TD>
 +
      <TD abp="628"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461220" abp="629">BBa_K1461220</A></TD>
 +
      <TD abp="630">Generator</TD>
 +
      <TD abp="631">ecf11-induced GFP generator</TD>
 +
      <TD width="100" abp="632">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="633">963</TD>
 +
      <TD abp="634"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="635">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="636">
 +
      <INPUT name="part" type="hidden" value="32719" abp="637">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="638">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="639">
 +
    <TR abp="640">
 +
      <TD abp="641"> </TD>
 +
      <TD abp="642"> </TD>
 +
      <TD abp="643"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461221" abp="644">BBa_K1461221</A></TD>
 +
      <TD abp="645">Generator</TD>
 +
      <TD abp="646">ecf20 generator (positive feedback)</TD>
 +
      <TD width="100" abp="647">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="648">825</TD>
 +
      <TD abp="649"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="650">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="651">
 +
      <INPUT name="part" type="hidden" value="32720" abp="652">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="653">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="654">
 +
    <TR abp="655">
 +
      <TD abp="656"> </TD>
 +
      <TD abp="657"> </TD>
 +
      <TD abp="658"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461222" abp="659">BBa_K1461222</A></TD>
 +
      <TD abp="660">Generator</TD>
 +
      <TD abp="661">ecf11 generator (positive feedback)</TD>
 +
      <TD width="100" abp="662">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="663">852</TD>
 +
      <TD abp="664"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="665">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="666">
 +
      <INPUT name="part" type="hidden" value="32730" abp="667">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="668">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="669">
 +
    <TR abp="670">
 +
      <TD abp="671"> </TD>
 +
      <TD abp="672"> </TD>
 +
      <TD abp="673"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461223" abp="674">BBa_K1461223</A></TD>
 +
      <TD abp="675">Generator</TD>
 +
      <TD abp="676">anti ecf20 generator (pLac)</TD>
 +
      <TD width="100" abp="677">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="678">795</TD>
 +
      <TD abp="679"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="680">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="681">
 +
      <INPUT name="part" type="hidden" value="32732" abp="682">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="683">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="684">
 +
    <TR abp="685">
 +
      <TD abp="686"> </TD>
 +
      <TD abp="687"> </TD>
 +
      <TD abp="688"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461224" abp="689">BBa_K1461224</A></TD>
 +
      <TD abp="690">Generator</TD>
 +
      <TD abp="691">anti ecf11 generator (pLac)</TD>
 +
      <TD width="100" abp="692">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="693">1026</TD>
 +
      <TD abp="694"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="695">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="696">
 +
      <INPUT name="part" type="hidden" value="32800" abp="697">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="698">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="699">
 +
    <TR abp="700">
 +
      <TD abp="701"> </TD>
 +
      <TD abp="702"> </TD>
 +
      <TD abp="703"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461225" abp="704">BBa_K1461225</A></TD>
 +
      <TD abp="705">Measurement</TD>
 +
      <TD abp="706">ecf20 promoter measurement system</TD>
 +
      <TD width="100" abp="707">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="708">1846</TD>
 +
      <TD abp="709"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="710">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="711">
 +
      <INPUT name="part" type="hidden" value="32802" abp="712">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="713">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="714">
 +
    <TR abp="715">
 +
      <TD abp="716"> </TD>
 +
      <TD abp="717"> </TD>
 +
      <TD abp="718"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461226" abp="719">BBa_K1461226</A></TD>
 +
      <TD abp="720">Measurement</TD>
 +
      <TD abp="721">ecf11 promoter measurement system</TD>
 +
      <TD width="100" abp="722">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="723">1873</TD>
 +
      <TD abp="724"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="725">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="726">
 +
      <INPUT name="part" type="hidden" value="32811" abp="727">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="728">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="729">
 +
    <TR abp="790">
 +
      <TD abp="791"> </TD>
 +
      <TD abp="792"> </TD>
 +
      <TD abp="793"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461231" abp="794">BBa_K1461231</A></TD>
 +
      <TD abp="795">Measurement</TD>
 +
      <TD abp="796">arabinose-induced GFP and taRNA generator</TD>
 +
      <TD width="100" abp="797">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="798">1407</TD>
 +
      <TD abp="799"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="800">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="801">
 +
      <INPUT name="part" type="hidden" value="32817" abp="802">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="803">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="804">
 +
    <TR abp="805">
 +
      <TD abp="806"> </TD>
 +
      <TD abp="807"> </TD>
 +
      <TD abp="808"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461232" abp="809">BBa_K1461232</A></TD>
 +
      <TD abp="810">Measurement</TD>
 +
      <TD abp="811">single counter</TD>
 +
      <TD width="100" abp="812">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="813">1312</TD>
 +
      <TD abp="814"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="815">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="816">
 +
      <INPUT name="part" type="hidden" value="32818" abp="817">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="818">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="819">
 +
    <TR abp="820">
 +
      <TD abp="821"> </TD>
 +
      <TD abp="822"> </TD>
 +
      <TD abp="823"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461233" abp="824">BBa_K1461233</A></TD>
 +
      <TD abp="825">Measurement</TD>
 +
      <TD abp="826">ecf20 promoter mesurement system (positive feedback)</TD>
 +
      <TD width="100" abp="827">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="828">2679</TD>
 +
      <TD abp="829"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="830">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="831">
 +
      <INPUT name="part" type="hidden" value="32819" abp="832">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="833">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="834">
 +
    <TR abp="835">
 +
      <TD abp="836"> </TD>
 +
      <TD abp="837"> </TD>
 +
      <TD abp="838"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461234" abp="839">BBa_K1461234</A></TD>
 +
      <TD abp="840">Measurement</TD>
 +
      <TD abp="841">ecf11 promoter mesurement system (positive feedback) </TD>
 +
      <TD width="100" abp="842">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="843">2733</TD>
 +
      <TD abp="844"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="845">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="846">
 +
      <INPUT name="part" type="hidden" value="32820" abp="847">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="848">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="849">
 +
    <TR abp="850">
 +
      <TD abp="851"> </TD>
 +
      <TD abp="852"> </TD>
 +
      <TD abp="853"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461235" abp="854">BBa_K1461235</A></TD>
 +
      <TD abp="855">Measurement</TD>
 +
      <TD abp="856">resettable counter device using ecf20 (w/o crRBS, taRNA)</TD>
 +
      <TD width="100" abp="857">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="858">3482</TD>
 +
      <TD abp="859"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="860">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="861">
 +
      <INPUT name="part" type="hidden" value="32821" abp="862">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="863">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="864">
 +
    <TR abp="865">
 +
      <TD abp="866"> </TD>
 +
      <TD abp="867"> </TD>
 +
      <TD abp="868"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461236" abp="869">BBa_K1461236</A></TD>
 +
      <TD abp="870">Measurement</TD>
 +
      <TD abp="871">resettable counter device using ecf11 (w/o crRBS, taRNA) </TD>
 +
      <TD width="100" abp="872">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="873">3767</TD>
 +
      <TD abp="874"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="875">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="876">
 +
      <INPUT name="part" type="hidden" value="32822" abp="877">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="878">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="879">
 +
    <TR abp="880">
 +
      <TD abp="881"> </TD>
 +
      <TD abp="882"> </TD>
 +
      <TD abp="883"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461237" abp="884">BBa_K1461237</A></TD>
 +
      <TD abp="885">Measurement</TD>
 +
      <TD abp="886">sigma factors cross-talk assay 1</TD>
 +
      <TD width="100" abp="887">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="888">1873</TD>
 +
      <TD abp="889"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="890">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="891">
 +
      <INPUT name="part" type="hidden" value="32823" abp="892">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="893">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="894">
 +
    <TR abp="895">
 +
      <TD abp="896"> </TD>
 +
      <TD abp="897"> </TD>
 +
      <TD abp="898"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461238" abp="899">BBa_K1461238</A></TD>
 +
      <TD abp="900">Measurement</TD>
 +
      <TD abp="901">sigma factors cross-talk assay 2</TD>
 +
      <TD width="100" abp="902">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="903">1846</TD>
 +
      <TD abp="904"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="905">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="906">
 +
      <INPUT name="part" type="hidden" value="32826" abp="907">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="908">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="909">
 +
    <TR abp="730">
 +
      <TD abp="731"> </TD>
 +
      <TD abp="732">  </TD>
 +
      <TD abp="733"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461227" abp="734">BBa_K1461227</A></TD>
 +
      <TD abp="735">Project</TD>
 +
      <TD abp="736">resettable single counter system using ecf20</TD>
 +
      <TD width="100" abp="737">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="738">2671</TD>
 +
      <TD abp="739"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="740">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="741">
 +
      <INPUT name="part" type="hidden" value="32813" abp="742">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="743">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="744">
 +
    <TR abp="745">
 +
      <TD abp="746"> </TD>
 +
      <TD abp="747"> </TD>
 +
      <TD abp="748"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461228" abp="749">BBa_K1461228</A></TD>
 +
      <TD abp="750">Project</TD>
 +
      <TD abp="751">reset function using ecf20</TD>
 +
      <TD width="100" abp="752">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="753">2576</TD>
 +
      <TD abp="754"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="755">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="756">
 +
      <INPUT name="part" type="hidden" value="32814" abp="757">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="758">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="759">
 +
    <TR abp="760">
 +
      <TD abp="761"> </TD>
 +
      <TD abp="762"> </TD>
 +
      <TD abp="763"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461229" abp="764">BBa_K1461229</A></TD>
 +
      <TD abp="765">Project</TD>
 +
      <TD abp="766">resettable single counter system using ecf11</TD>
 +
      <TD width="100" abp="767">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="768">2834</TD>
 +
      <TD abp="769"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="770">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="771">
 +
      <INPUT name="part" type="hidden" value="32815" abp="772">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="773">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="774">
 +
    <TR abp="775">
 +
      <TD abp="776"> </TD>
 +
      <TD abp="777"> </TD>
 +
      <TD abp="778"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461230" abp="779">BBa_K1461230</A></TD>
 +
      <TD abp="780">Project</TD>
 +
      <TD abp="781">reset function using ecf11</TD>
 +
      <TD width="100" abp="782">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="783">2929</TD>
 +
      <TD abp="784"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="785">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="786">
 +
      <INPUT name="part" type="hidden" value="32816" abp="787">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="788">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="789">
 +
    <TR abp="910">
 +
      <TD abp="911"> </TD>
 +
      <TD abp="912"> </TD>
 +
      <TD abp="913"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461239" abp="914">BBa_K1461239</A></TD>
 +
      <TD abp="915">Project</TD>
 +
      <TD abp="916">σ-Re Counter (double, using ecf20)</TD>
 +
      <TD width="100" abp="917">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="918">3821</TD>
 +
      <TD abp="919"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="920">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="921">
 +
      <INPUT name="part" type="hidden" value="32827" abp="922">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="923">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="924">
 +
    <TR abp="925">
 +
      <TD abp="926"> </TD>
 +
      <TD abp="927"> </TD>
 +
      <TD abp="928"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461240" abp="929">BBa_K1461240</A></TD>
 +
      <TD abp="930">Project</TD>
 +
      <TD abp="931">σ-Re Counter (double, using ecf11)</TD>
 +
      <TD width="100" abp="932">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="933">4106</TD>
 +
      <TD abp="934"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="935">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="936">
 +
      <INPUT name="part" type="hidden" value="32829" abp="937">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="938">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="939">
 +
    <TR abp="940">
 +
      <TD abp="941"> </TD>
 +
      <TD abp="942"> </TD>
 +
      <TD abp="943"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461241" abp="944">BBa_K1461241</A></TD>
 +
      <TD abp="945">Project</TD>
 +
      <TD abp="946">σ-Re Counter (triple)</TD>
 +
      <TD width="100" abp="947">Takefumi Yoshikawa</TD>
 +
      <TD align="right" abp="948">6615</TD>
 +
      <TD abp="949"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="950">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="951">
 +
      <INPUT name="part" type="hidden" value="32571" abp="952">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="953">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="954">
 +
    <TR abp="280">
 +
      <TD abp="281"> </TD>
 +
      <TD abp="282">  </TD>
 +
      <TD abp="283"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461104" abp="284">BBa_K1461104</A></TD>
 +
      <TD abp="285">Regulatory</TD>
 +
      <TD abp="286">EpCAM promoter</TD>
 +
      <TD width="100" abp="287">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="288"> </TD>
 +
      <TD abp="289"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="290">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="291">
 +
      <INPUT name="part" type="hidden" value="32405" abp="292">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="293">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="294">
 +
    <TR abp="295">
 +
      <TD abp="296"> </TD>
 +
      <TD abp="297">  </TD>
 +
      <TD abp="298"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461105" abp="299">BBa_K1461105</A></TD>
 +
      <TD abp="300">Regulatory</TD>
 +
      <TD abp="301">EpCAM promoter(mutant)</TD>
 +
      <TD width="100" abp="302">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="303"> </TD>
 +
      <TD abp="304"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="305">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="306">
 +
      <INPUT name="part" type="hidden" value="32404" abp="307">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="308">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="309">
 +
    <TR abp="310">
 +
      <TD abp="311"> </TD>
 +
      <TD abp="312"> </TD>
 +
      <TD abp="313"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461107" abp="314">BBa_K1461107</A></TD>
 +
      <TD abp="315">Regulatory</TD>
 +
      <TD abp="316">EGP-2</TD>
 +
      <TD width="100" abp="317">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="318"> </TD>
 +
      <TD abp="319"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="320">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="321">
 +
      <INPUT name="part" type="hidden" value="32539" abp="322">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="323">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="324">
 +
    <TR abp="220">
 +
      <TD abp="221"> </TD>
 +
      <TD abp="222">  </TD>
 +
      <TD abp="223"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461100" abp="224">BBa_K1461100</A></TD>
 +
      <TD abp="225">RNA</TD>
 +
      <TD abp="226">miR-142-3p target binding site</TD>
 +
      <TD width="100" abp="227">ドリキャス❤</TD>
 +
      <TD align="right" abp="228">25</TD>
 +
      <TD abp="229"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="230">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="231">
 +
      <INPUT name="part" type="hidden" value="32402" abp="232">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="233">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="234">
 +
    <TR abp="235">
 +
      <TD abp="236"> </TD>
 +
      <TD abp="237">  </TD>
 +
      <TD abp="238"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461101" abp="239">BBa_K1461101</A></TD>
 +
      <TD abp="240">RNA</TD>
 +
      <TD abp="241">miR-142-5p target bindng site</TD>
 +
      <TD width="100" abp="242">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="243">23</TD>
 +
      <TD abp="244"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="245">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="246">
 +
      <INPUT name="part" type="hidden" value="32658" abp="247">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="248">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="249">
 +
    <TR abp="250">
 +
      <TD abp="251"> </TD>
 +
      <TD abp="252"> </TD>
 +
      <TD abp="253"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461102" abp="254">BBa_K1461102</A></TD>
 +
      <TD abp="255">RNA</TD>
 +
      <TD abp="256">miRNA-142 binding site control1</TD>
 +
      <TD width="100" abp="257">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="258">23</TD>
 +
      <TD abp="259"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="260">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="261">
 +
      <INPUT name="part" type="hidden" value="32659" abp="262">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="263">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="264">
 +
    <TR abp="265">
 +
      <TD abp="266"> </TD>
 +
      <TD abp="267"> </TD>
 +
      <TD abp="268"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461103" abp="269">BBa_K1461103</A></TD>
 +
      <TD abp="270">RNA</TD>
 +
      <TD abp="271">miRNA-142 binding site control2</TD>
 +
      <TD width="100" abp="272">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="273">25</TD>
 +
      <TD abp="274"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="275">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="276">
 +
      <INPUT name="part" type="hidden" value="32403" abp="277">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="278">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="279">
 +
    <TR abp="1045">
 +
      <TD abp="1046"> </TD>
 +
      <TD abp="1047"> </TD>
 +
      <TD abp="1048"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461306" abp="1049">BBa_K1461306</A></TD>
 +
      <TD abp="1050">Translational_Unit</TD>
 +
      <TD abp="1051">pCMV-RBS-GFP-4 miR 142 3p site-d.term</TD>
 +
      <TD width="100" abp="1052">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1053">1595</TD>
 +
      <TD abp="1054"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1055">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1056">
 +
      <INPUT name="part" type="hidden" value="32639" abp="1057">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1058">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1059">
 +
    <TR abp="1060">
 +
      <TD abp="1061"> </TD>
 +
      <TD abp="1062"> </TD>
 +
      <TD abp="1063"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461307" abp="1064">BBa_K1461307</A></TD>
 +
      <TD abp="1065">Translational_Unit</TD>
 +
      <TD abp="1066">pCMV-RBS-GFP-4 miR 142 5p site-d.term</TD>
 +
      <TD width="100" abp="1067">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1068">1587</TD>
 +
      <TD abp="1069"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1070">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1071">
 +
      <INPUT name="part" type="hidden" value="32640" abp="1072">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1073">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1074">
 +
    <TR abp="1075">
 +
      <TD abp="1076"> </TD>
 +
      <TD abp="1077"> </TD>
 +
      <TD abp="1078"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461308" abp="1079">BBa_K1461308</A></TD>
 +
      <TD abp="1080">Translational_Unit</TD>
 +
      <TD abp="1081">pCMV-RBS-GFP-4 miR 142 3p non-binding sites-d.term</TD>
 +
      <TD width="100" abp="1082">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1083">1589</TD>
 +
      <TD abp="1084"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1085">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1086">
 +
      <INPUT name="part" type="hidden" value="32641" abp="1087">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1088">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1089">
 +
    <TR abp="1090">
 +
      <TD abp="1091"> </TD>
 +
      <TD abp="1092"> </TD>
 +
      <TD abp="1093"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461309" abp="1094">BBa_K1461309</A></TD>
 +
      <TD abp="1095">Translational_Unit</TD>
 +
      <TD abp="1096">pCMV-RBS-GFP-4 miR 142 5p non-bindgin sites-d.term</TD>
 +
      <TD width="100" abp="1097">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1098">1595</TD>
 +
      <TD abp="1099"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1100">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1101">
 +
      <INPUT name="part" type="hidden" value="32642" abp="1102">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1103">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1104">
 +
    <TR abp="1105">
 +
      <TD abp="1106"> </TD>
 +
      <TD abp="1107"> </TD>
 +
      <TD abp="1108"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461310" abp="1109">BBa_K1461310</A></TD>
 +
      <TD abp="1110">Translational_Unit</TD>
 +
      <TD abp="1111">pCMV-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term</TD>
 +
      <TD width="100" abp="1112">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1113">1591</TD>
 +
      <TD abp="1114"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1115">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1116">
 +
      <INPUT name="part" type="hidden" value="32643" abp="1117">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1118">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1119">
 +
    <TR abp="1120">
 +
      <TD abp="1121"> </TD>
 +
      <TD abp="1122"> </TD>
 +
      <TD abp="1123"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461311" abp="1124">BBa_K1461311</A></TD>
 +
      <TD abp="1125">Translational_Unit</TD>
 +
      <TD abp="1126">pCMV-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term</TD>
 +
      <TD width="100" abp="1127">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1128">1591</TD>
 +
      <TD abp="1129"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1130">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1131">
 +
      <INPUT name="part" type="hidden" value="32645" abp="1132">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1133">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1134">
 +
    <TR abp="1135">
 +
      <TD abp="1136"> </TD>
 +
      <TD abp="1137"> </TD>
 +
      <TD abp="1138"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461312" abp="1139">BBa_K1461312</A></TD>
 +
      <TD abp="1140">Translational_Unit</TD>
 +
      <TD abp="1141">pEpCAM-RBS-GFP-4 miR 142 3p site-d.term</TD>
 +
      <TD width="100" abp="1142">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1143">738</TD>
 +
      <TD abp="1144"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1145">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1146">
 +
      <INPUT name="part" type="hidden" value="32646" abp="1147">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1148">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1149">
 +
    <TR abp="1150">
 +
      <TD abp="1151"> </TD>
 +
      <TD abp="1152"> </TD>
 +
      <TD abp="1153"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461313" abp="1154">BBa_K1461313</A></TD>
 +
      <TD abp="1155">Translational_Unit</TD>
 +
      <TD abp="1156">pEpCAM-RBS-GFP-4 miR 142 5p site-d.term</TD>
 +
      <TD width="100" abp="1157">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1158"> </TD>
 +
      <TD abp="1159"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1160">
 +
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 +
      <INPUT name="part" type="hidden" value="32647" abp="1162">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1163">
 +
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 +
    <TR abp="1165">
 +
      <TD abp="1166"> </TD>
 +
      <TD abp="1167"> </TD>
 +
      <TD abp="1168"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461314" abp="1169">BBa_K1461314</A></TD>
 +
      <TD abp="1170">Translational_Unit</TD>
 +
      <TD abp="1171">pEpCAM-RBS-GFP-4 miR 142 3p non-bindng site-d.term</TD>
 +
      <TD width="100" abp="1172">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1173"> </TD>
 +
      <TD abp="1174"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1175">
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 +
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 +
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 +
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 +
    <TR abp="1180">
 +
      <TD abp="1181"> </TD>
 +
      <TD abp="1182"> </TD>
 +
      <TD abp="1183"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461315" abp="1184">BBa_K1461315</A></TD>
 +
      <TD abp="1185">Translational_Unit</TD>
 +
      <TD abp="1186">pEpCAM-RBS-GFP-4 miR 142 5p non-bindng site-d.term</TD>
 +
      <TD width="100" abp="1187">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1188"> </TD>
 +
      <TD abp="1189"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1190">
 +
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 +
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 +
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 +
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 +
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 +
    <TR abp="1195">
 +
      <TD abp="1196"> </TD>
 +
      <TD abp="1197"> </TD>
 +
      <TD abp="1198"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461316" abp="1199">BBa_K1461316</A></TD>
 +
      <TD abp="1200">Translational_Unit</TD>
 +
      <TD abp="1201">pEpCAM-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term</TD>
 +
      <TD width="100" abp="1202">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1203"> </TD>
 +
      <TD abp="1204"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1205">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1206">
 +
      <INPUT name="part" type="hidden" value="32650" abp="1207">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1208">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1209">
 +
    <TR abp="1210">
 +
      <TD abp="1211"> </TD>
 +
      <TD abp="1212"> </TD>
 +
      <TD abp="1213"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461317" abp="1214">BBa_K1461317</A></TD>
 +
      <TD abp="1215">Translational_Unit</TD>
 +
      <TD abp="1216">pEpCAM-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term</TD>
 +
      <TD width="100" abp="1217">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1218"> </TD>
 +
      <TD abp="1219"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1220">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1221">
 +
      <INPUT name="part" type="hidden" value="32651" abp="1222">
 +
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 +
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 +
    <TR abp="1225">
 +
      <TD abp="1226"> </TD>
 +
      <TD abp="1227"> </TD>
 +
      <TD abp="1228"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461318" abp="1229">BBa_K1461318</A></TD>
 +
      <TD abp="1230">Translational_Unit</TD>
 +
      <TD abp="1231">pEpCAM(mutant)-RBS-GFP-4 miR 142 3p site-d.term</TD>
 +
      <TD width="100" abp="1232">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1233"> </TD>
 +
      <TD abp="1234"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1235">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1236">
 +
      <INPUT name="part" type="hidden" value="32652" abp="1237">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1238">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1239">
 +
    <TR abp="1240">
 +
      <TD abp="1241"> </TD>
 +
      <TD abp="1242"> </TD>
 +
      <TD abp="1243"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461319" abp="1244">BBa_K1461319</A></TD>
 +
      <TD abp="1245">Translational_Unit</TD>
 +
      <TD abp="1246">pEpCAM(mutant)-RBS-GFP-4 miR 142 5p site-d.term</TD>
 +
      <TD width="100" abp="1247">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1248"> </TD>
 +
      <TD abp="1249"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1250">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1251">
 +
      <INPUT name="part" type="hidden" value="32654" abp="1252">
 +
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 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1254">
 +
    <TR abp="1255">
 +
      <TD abp="1256"> </TD>
 +
      <TD abp="1257"> </TD>
 +
      <TD abp="1258"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461320" abp="1259">BBa_K1461320</A></TD>
 +
      <TD abp="1260">Translational_Unit</TD>
 +
      <TD abp="1261">pEpCAM(mutant)-RBS-GFP-4 miR 142 3p non-bindng site-d.term</TD>
 +
      <TD width="100" abp="1262">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1263"> </TD>
 +
      <TD abp="1264"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1265">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1266">
 +
      <INPUT name="part" type="hidden" value="32655" abp="1267">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1268">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1269">
 +
    <TR abp="1270">
 +
      <TD abp="1271"> </TD>
 +
      <TD abp="1272"> </TD>
 +
      <TD abp="1273"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461321" abp="1274">BBa_K1461321</A></TD>
 +
      <TD abp="1275">Translational_Unit</TD>
 +
      <TD abp="1276">pEpCAM(mutant)-RBS-GFP-4 miR 142 5p non-bindng site-d.term</TD>
 +
      <TD width="100" abp="1277">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1278"> </TD>
 +
      <TD abp="1279"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1280">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1281">
 +
      <INPUT name="part" type="hidden" value="32656" abp="1282">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1283">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1284">
 +
    <TR abp="1285">
 +
      <TD abp="1286"> </TD>
 +
      <TD abp="1287"> </TD>
 +
      <TD abp="1288"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461322" abp="1289">BBa_K1461322</A></TD>
 +
      <TD abp="1290">Translational_Unit</TD>
 +
      <TD abp="1291">pEpCAM(mutant)-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term</TD>
 +
      <TD width="100" abp="1292">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1293"> </TD>
 +
      <TD abp="1294"></TD>
 +
      <FORM action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=UT-Tokyo" enctype="multipart/form-data" method="post" abp="1295">
 +
      </FORM>
 +
      <INPUT name="Editing2" type="hidden" value="Editing" abp="1296">
 +
      <INPUT name="part" type="hidden" value="32657" abp="1297">
 +
      <INPUT name="pgroup" type="hidden" value="iGEM2014" abp="1298">
 +
      <INPUT name="group" type="hidden" value="UT-Tokyo" abp="1299">
 +
    <TR abp="1300">
 +
      <TD abp="1301"> </TD>
 +
      <TD abp="1302"> </TD>
 +
      <TD abp="1303"><A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461323" abp="1304">BBa_K1461323</A></TD>
 +
      <TD abp="1305">Translational_Unit</TD>
 +
      <TD abp="1306">pEpCAM(mutant)-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term</TD>
 +
      <TD width="100" abp="1307">Shunsuke Sumi</TD>
 +
      <TD align="right" abp="1308"> </TD>
 +
      <TD abp="1309"></TD>
 +
    </TR>
 +
    </TBODY>
 +
  </TABLE>
 +
</div>
 +
<div id = "Lab-3">
<div id="0714"><h3>7,14,2014</h3></div>
<div id="0714"><h3>7,14,2014</h3></div>
<h4>Lab member</h4> <p>Yoshikawa,Nakashima</p>
<h4>Lab member</h4> <p>Yoshikawa,Nakashima</p>
Line 1,793: Line 3,094:
</p>
</p>
<h5>making M9 medium</h5>
<h5>making M9 medium</h5>
 +
</div>

Revision as of 17:13, 17 October 2014

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Name Type Description Designer Length
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461000" abp="49">BBa_K1461000</A> RBS crRBS Takefumi Yoshikawa 52
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461001" abp="64">BBa_K1461001</A> Regulatory ecf20 promoter Takefumi Yoshikawa 80
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461002" abp="79">BBa_K1461002</A> Regulatory ecf11 promoter Takefumi Yoshikawa 80
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461003" abp="94">BBa_K1461003</A> RNA taRNA Takefumi Yoshikawa 71
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461004" abp="109">BBa_K1461004</A> Coding ecf20 Takefumi Yoshikawa 582
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461005" abp="124">BBa_K1461005</A> Coding ecf11 Takefumi Yoshikawa 609
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461006" abp="139">BBa_K1461006</A> Coding anti ecf20 Takefumi Yoshikawa 432
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461007" abp="154">BBa_K1461007</A> Coding anti ecf11 Takefumi Yoshikawa 663 
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</TBODY>
Name Type Description Designer Length
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461008" abp="194">BBa_K1461008</A> Coding GFP (AAV tag +) Takefumi Yoshikawa 742
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461009" abp="209">BBa_K1461009</A> Coding GFP (AAV tag +) Takefumi Yoshikawa 742
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461200" abp="329">BBa_K1461200</A> Composite taRNA (+d.term) Takefumi Yoshikawa 208
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461201" abp="344">BBa_K1461201</A> Composite ecf20 (+RBS, +d.term) Takefumi Yoshikawa 737
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461202" abp="359">BBa_K1461202</A> Composite ecf11 (+RBS, +d.term) Takefumi Yoshikawa 764
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461203" abp="374">BBa_K1461203</A> Composite cr-ecf20 (+RBS, +d.term) Takefumi Yoshikawa 777
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461204" abp="389">BBa_K1461204</A> Composite cr-ecf11 (+RBS, +d.term) Takefumi Yoshikawa 804
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461205" abp="404">BBa_K1461205</A> Composite cr-GFP (+RBS, +d.term) Takefumi Yoshikawa 915
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461206" abp="419">BBa_K1461206</A> Composite anti ecf20 (+RBS, +d.term) Takefumi Yoshikawa 587
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461207" abp="434">BBa_K1461207</A> Composite anti ecf11 (+RBS, +d.term) Takefumi Yoshikawa 818
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461300" abp="959">BBa_K1461300</A> Composite RBS-GFP-4miR-142-3p bindng sites-d.term Shunsuke Sumi 1007
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461301" abp="974">BBa_K1461301</A> Composite RBS-GFP-4 miR-142-5p bindng sites-d.term Shunsuke Sumi 999
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461302" abp="989">BBa_K1461302</A> Composite RBS-GFP-miR 142 3p non-binding site-d.term Shunsuke Sumi 1001
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461303" abp="1004">BBa_K1461303</A> Composite RBS-GFP-miR 142 5p non-binding site-d.term Shunsuke Sumi 1007
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461304" abp="1019">BBa_K1461304</A> Composite RBS-GFP-2miR 142 3p bindng sites-2miR 142 5p bindng sites-d.term Shunsuke Sumi 1003
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461305" abp="1034">BBa_K1461305</A> Composite RBS-GFP-2miR 142 3p non-bindng sites-2miR 142 5p non-bindng sites-d.term Shunsuke Sumi 1003
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461208" abp="449">BBa_K1461208</A> Generator taRNA (arabinose-induced) (+d.term) Takefumi Yoshikawa 346
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461209" abp="464">BBa_K1461209</A> Generator arabinose-induced ecf20 generator Takefumi Yoshikawa 875
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461210" abp="479">BBa_K1461210</A> Generator constitutive ecf20 generator Takefumi Yoshikawa 780
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461211" abp="494">BBa_K1461211</A> Generator arabinose-induced ecf11 generator Takefumi Yoshikawa 902
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461212" abp="509">BBa_K1461212</A> Generator constitutive ecf11 generator Takefumi Yoshikawa 807
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461213" abp="524">BBa_K1461213</A> Generator arabinose-induced cr-ecf20 generator Takefumi Yoshikawa 915
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461214" abp="539">BBa_K1461214</A> Generator constitutive cr-ecf20 generator Takefumi Yoshikawa 820
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461215" abp="554">BBa_K1461215</A> Generator arabinose-induced cr-ecf11 generator Takefumi Yoshikawa 942
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461216" abp="569">BBa_K1461216</A> Generator constitutive cr-ecf11 generator Takefumi Yoshikawa 847
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461217" abp="584">BBa_K1461217</A> Generator arabinose-induced cr-GFP generator Takefumi Yoshikawa 1053
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461218" abp="599">BBa_K1461218</A> Generator constitutive cr-GFP generator Takefumi Yoshikawa 958
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461219" abp="614">BBa_K1461219</A> Generator ecf20-induced GFP generator Takefumi Yoshikawa 963
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461220" abp="629">BBa_K1461220</A> Generator ecf11-induced GFP generator Takefumi Yoshikawa 963
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461221" abp="644">BBa_K1461221</A> Generator ecf20 generator (positive feedback) Takefumi Yoshikawa 825
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461222" abp="659">BBa_K1461222</A> Generator ecf11 generator (positive feedback) Takefumi Yoshikawa 852
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461223" abp="674">BBa_K1461223</A> Generator anti ecf20 generator (pLac) Takefumi Yoshikawa 795
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461224" abp="689">BBa_K1461224</A> Generator anti ecf11 generator (pLac) Takefumi Yoshikawa 1026
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461225" abp="704">BBa_K1461225</A> Measurement ecf20 promoter measurement system Takefumi Yoshikawa 1846
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461226" abp="719">BBa_K1461226</A> Measurement ecf11 promoter measurement system Takefumi Yoshikawa 1873
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461231" abp="794">BBa_K1461231</A> Measurement arabinose-induced GFP and taRNA generator Takefumi Yoshikawa 1407
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461232" abp="809">BBa_K1461232</A> Measurement single counter Takefumi Yoshikawa 1312
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461233" abp="824">BBa_K1461233</A> Measurement ecf20 promoter mesurement system (positive feedback) Takefumi Yoshikawa 2679
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461234" abp="839">BBa_K1461234</A> Measurement ecf11 promoter mesurement system (positive feedback) Takefumi Yoshikawa 2733
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461235" abp="854">BBa_K1461235</A> Measurement resettable counter device using ecf20 (w/o crRBS, taRNA) Takefumi Yoshikawa 3482
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461236" abp="869">BBa_K1461236</A> Measurement resettable counter device using ecf11 (w/o crRBS, taRNA) Takefumi Yoshikawa 3767
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461237" abp="884">BBa_K1461237</A> Measurement sigma factors cross-talk assay 1 Takefumi Yoshikawa 1873
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461238" abp="899">BBa_K1461238</A> Measurement sigma factors cross-talk assay 2 Takefumi Yoshikawa 1846
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461227" abp="734">BBa_K1461227</A> Project resettable single counter system using ecf20 Takefumi Yoshikawa 2671
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461228" abp="749">BBa_K1461228</A> Project reset function using ecf20 Takefumi Yoshikawa 2576
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461229" abp="764">BBa_K1461229</A> Project resettable single counter system using ecf11 Takefumi Yoshikawa 2834
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461230" abp="779">BBa_K1461230</A> Project reset function using ecf11 Takefumi Yoshikawa 2929
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461239" abp="914">BBa_K1461239</A> Project σ-Re Counter (double, using ecf20) Takefumi Yoshikawa 3821
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461240" abp="929">BBa_K1461240</A> Project σ-Re Counter (double, using ecf11) Takefumi Yoshikawa 4106
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461241" abp="944">BBa_K1461241</A> Project σ-Re Counter (triple) Takefumi Yoshikawa 6615
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461104" abp="284">BBa_K1461104</A> Regulatory EpCAM promoter Shunsuke Sumi  
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461105" abp="299">BBa_K1461105</A> Regulatory EpCAM promoter(mutant) Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461107" abp="314">BBa_K1461107</A> Regulatory EGP-2 Shunsuke Sumi  
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461100" abp="224">BBa_K1461100</A> RNA miR-142-3p target binding site ドリキャス❤ 25
     <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461101" abp="239">BBa_K1461101</A> RNA miR-142-5p target bindng site Shunsuke Sumi 23
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461102" abp="254">BBa_K1461102</A> RNA miRNA-142 binding site control1 Shunsuke Sumi 23
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461103" abp="269">BBa_K1461103</A> RNA miRNA-142 binding site control2 Shunsuke Sumi 25
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461306" abp="1049">BBa_K1461306</A> Translational_Unit pCMV-RBS-GFP-4 miR 142 3p site-d.term Shunsuke Sumi 1595
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461307" abp="1064">BBa_K1461307</A> Translational_Unit pCMV-RBS-GFP-4 miR 142 5p site-d.term Shunsuke Sumi 1587
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461308" abp="1079">BBa_K1461308</A> Translational_Unit pCMV-RBS-GFP-4 miR 142 3p non-binding sites-d.term Shunsuke Sumi 1589
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461309" abp="1094">BBa_K1461309</A> Translational_Unit pCMV-RBS-GFP-4 miR 142 5p non-bindgin sites-d.term Shunsuke Sumi 1595
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461310" abp="1109">BBa_K1461310</A> Translational_Unit pCMV-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term Shunsuke Sumi 1591
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461311" abp="1124">BBa_K1461311</A> Translational_Unit pCMV-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term Shunsuke Sumi 1591
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461312" abp="1139">BBa_K1461312</A> Translational_Unit pEpCAM-RBS-GFP-4 miR 142 3p site-d.term Shunsuke Sumi 738
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461313" abp="1154">BBa_K1461313</A> Translational_Unit pEpCAM-RBS-GFP-4 miR 142 5p site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461314" abp="1169">BBa_K1461314</A> Translational_Unit pEpCAM-RBS-GFP-4 miR 142 3p non-bindng site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461315" abp="1184">BBa_K1461315</A> Translational_Unit pEpCAM-RBS-GFP-4 miR 142 5p non-bindng site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461316" abp="1199">BBa_K1461316</A> Translational_Unit pEpCAM-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461317" abp="1214">BBa_K1461317</A> Translational_Unit pEpCAM-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461318" abp="1229">BBa_K1461318</A> Translational_Unit pEpCAM(mutant)-RBS-GFP-4 miR 142 3p site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461319" abp="1244">BBa_K1461319</A> Translational_Unit pEpCAM(mutant)-RBS-GFP-4 miR 142 5p site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461320" abp="1259">BBa_K1461320</A> Translational_Unit pEpCAM(mutant)-RBS-GFP-4 miR 142 3p non-bindng site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461321" abp="1274">BBa_K1461321</A> Translational_Unit pEpCAM(mutant)-RBS-GFP-4 miR 142 5p non-bindng site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461322" abp="1289">BBa_K1461322</A> Translational_Unit pEpCAM(mutant)-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term Shunsuke Sumi  
    <A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461323" abp="1304">BBa_K1461323</A> Translational_Unit pEpCAM(mutant)-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term Shunsuke Sumi  

Contents

7,14,2014

Lab member

Yoshikawa,Nakashima


Contents

Making Plate Culture

Ampicillin *10
Chloramphenicol*6

7,15,2014

Lab member

Yoshikawa,Nakashima


Contents

Making DW 100mL


TF

4-17F(A-1), 4-4E(A-2), 3-19O(A-3), 3-4G(A-4), 4-1N(A-6), 3-3F(A-8), 4-13L(B-10)


7,16,2014

Lab member

Yoshikawa,Nakashima


Contents

A-2:No colony

TF

A-2(recovery)

preculture

A-1, A-3, A-4, A-6, A-8, B-10,Escherichia coli JM109(for competent cell)


7,17,2014

Lab member

Yoshikawa,Nakashima


Contents

A-2:There are something like colonies.

Making glycerol stock/miniprep

A-1, A-3, A-4, A-6, A-8
All samples:consentration is low.
B-10:disposed

making reagent

LB: 500mL
50mM CaCl2: 400mL
50mM CaCl2/ 20% glycerol: 200mL

preculture

A-1 #1
A-2 #1 #2
A-3 #1
A-4 #1
A-6 #1
A-8 #1
B-10 #1 #2
E. coli JM109（for competent cell）

  1. colony number


7,18,2014

Lab member

Yoshikawa,Nakashima


Contents

miniprep

A-1,A-3,A-6:from culture 2.5ml OK
A-4,A-8:from culture 1.5ml OK
A-2#1:made glycerol stock. Low consentration.
B-10#1:Low consentration.
A-2#2,B-10#2:did not grow


Making competent cell

100uL*120


making reagent

（2000×）Ampicillin 3ｍL　（100mg/mL）


7,22,2014

Lab member

Yoshikawa,Nakashima


Contents

Cut check

A-4 (81ng/μL):ES Cut,XP cut.Checked in 1.5h, 2h, 2.5h, 3h:OK
Left:1kbp Ladder/A-4(Control)/ES 1.5h/ES 2h/ES 2.5h/ES 3h
Right:1kbp Ladder/A-4(Control)/XP 1.5h/XP 2h/XP 2.5h/XP 3h
<img src="IMGP0022_20140722_1.JPG"/>


TF

We measured cfu of competent cell we made.
We used Efficiency Kit （RFP Construct on pSB1C3）in distribution Kit.

TF

50pg, 20pg, 10pg, 5pg

competent cell:25uL

Sprinkling:100uL


preculture

B-10 #3, A-4


7,23,2014

Lab member

Yoshikawa,Nakashima


Contents

plate check:Did not grow.


miniprep

A-4, B-10 #3(made glycerol stock):OK

Making plate

200ml,CP*10


7,28,2014

Lab member

Nakashima


Contents

digestion,gel extraction

A-6 SP, B-10 XP：disposed
100bp Ladder, A-6 SP, B-10 XP, NC
<img src="IMGP0033_20140728_1.JPG"/>
(wrote at 0731
A-6
band near 2kbp:SP cut or single cut
band near 1.5kbp:Plasmid)

colony PCR

A-6 #1, B-10 #1
1kbp Ladder, A-6, B-10, NC
<img src="IMGP0034_20140728_2.JPG"/>
A-6:OK,B-10:wrong

Measuring cfu

competent cell:50uL

7,29,2014

Lab member

Yoshikawa,Nakashima


Contents

Plate check:All Plates did not grow.


TF

B-10 by electroporation.

Measuring cfu

A-1:13,65,130pg

Electrophoresis check

1kbp Ladder, A-6(Plasmid), NC
<img src="IMGP0035_20140729_1.JPG"/>
There is band in 1500kbp,so 1500kbp in 0728 may be rest of cutting.

No contamination in Dye.


7,30,2014

Lab member

Yoshikawa,Nakashima


Contents

colony check

cfu:Did not grow
B-10:One colony

Cut check

We checked whether A-6 band in 0728 was rest of cutting.

A-1 SP cut:checked on different times.


colony PCR

B-10(from Plate made in 0729)
1kbp Ladder, 1.5h, 2h, 2.5h, B-10(colony PCR), NC
<img src="IMGP0042_20140730_1.JPG"/>
band near 800bp:RFP between Spe1 site and Pst1 site of A-1.
band near 2kbp:Backbone cut by SP cut
band near 3kbp:rest of cutting

1.5h:incompletely cut
2h,2.5h:OK
We decided 2h for digestion.
B-10:OK

preculture

B-10

7,31,2014

Lab member

Yoshikawa,Nakashima


Contents

miniprep

B-10 #1（from 140729 Plate）(made glycerol stock)

digestion,gel extraction

A-6 SP, B-10 XP

1kbp Ladder, A-6, B-10
<img src="IMGP0043_20140731_1.JPG"/>
B-10:incomplete cut
others:OK

ligation

C-7 (A-6 SP + B-10 XP)

8,1,2014

Lab member

Yoshikawa,Nakashima


Contents

TF

C-7（Chemical & EP）

ligation Check

PCR reaction primed with Universal Primer, using ligation products as template.
<img src="IMGP0044_20140801_1.JPG"/>
OK!

8,4,2014

Lab member

Yoshikawa,Nakashima


Contents

Plate check

No colony

digestion

A-6 SP, B-10 XP

Electrophoresis

1kbp Ladder, A-6, B-10
<img src="IMGP0045_20140804_1.JPG"/>
Incomplete cut.Disposed.

preculture

B-10

8,5,2014

Lab member

Yoshikawa,Nakashima


Contents

miniprep

B-10　→Sample Lost!　Bye bye, GFP!

Cut check

SP(A-1), XP(A-1), EX(A-4), ES(A-4)
2h, 2.5h, 3h

Elrctrophoresis

1kbp Ladder, A-1, A-1 SP(2h, 2.5h, 3h), A-1 XP(2h,2.5h, 3h), A-4,

A-4 EX(2h, 2.5h 3h), A-4 ES(2h, 2.5h, 3h), None, 1kbp Ladder

<img src="IMGP0046_20140805_1.JPG"/>

Incomplete cut.


preculture

A-1, A-4, B-10

8,6,2014

Lab member

Yoshikawa


Contents

miniprep

A-1, A-4, B-10

digestion, gel extraction

A-6 SP, B-10 XP

1kbp Ladder, A-6, B-10
<img src="IMGP0047_20140806_1.JPG"/>
A-6:OK
B-10:Incomplete cut
<img src="IMGP0048_20140806_2.JPG"/>

We chenged restriction enzyme.
B-10 XP
<img src="IMGP0051_20140806_3.JPG"/>
OK!
<img src="IMGP0060_20140806_4.JPG"/>

making gel

200ml


ligation

C-7（A-6 SP + B-10 XP）

TF

4-11L, C-7

8，7,2014

Lab member

Yoshikawa,Nakashima,Tara,Itoh,Tsukada


Contents

colony PCR

C-7
<img src="IMGP0061_20140807_1.JPG"/>
<img src="IMGP0062_20140807_2.JPG"/>
All bands are self ligation of backbone.

digestion, gel extraction

A-6 SP, B-10 XP

1kbp Ladder, B-10, A-6
<img src="IMGP0063_20140807_3.JPG"/>

ligation

C-7 (A-6 SP + B-10 XP)

Cut check

(O/N)

（enzyme-buffer) S-B, S-H*, S-M*, P*-FD, E-FD

  • Takara's product


8,8,2014

Lab member

Nakashima,Nakamura,Yamanaka,Yoshikawa(Fresh),Yoshikawa


Contents

Cut check sample Electrophoresis


1kbp Ladder, S-B, S-H*, S-M*, NC, E-FD, P*-FD
<img src="IMGP0063_20140808_1.JPG"/>
FD buffer is appropriate for SP cut.

digestion, gel extraction

A-6 SP, B-10 XP
<img src="IMGP0064_20140808_2.JPG"/>
<img src="IMGP0065_20140808_3.JPG"/>
OK!

ligation

C-7 (A-6 SP + B-10 XP)

TF

C-7,4-11L(culture in test tube)

overlap extension PCR →PCR clean up

1+2
anealing 57℃,extension 10 sec, 30 cycle.

100bp Ladder, Pecf11, Pecf20, crRBS, taRNA
<img src="IMGP0066_20140808_4.JPG"/>
OK!(low concentration)

making gel

200ml

8,9,2014

Lab member

Yoshikawa


Contents

Plate and test tube check

4-11L:OK!mixed with glycerol, and conserved in -80℃.
C-7:OK! conserved in refrigerator.

8,11,2014

Lab member

Yoshikawa,Nakashima,Yamanaka,Nakamura


Contents

colony PCR

C-7 #1~11

  1. 1~4, NC

<img src="IMGP0067_20140811_1.JPG"/>

  1. 5~11,NC

<img src="IMGP0069_20140811_2.JPG"/>

  1. 10 is OK!


overlap extension PCR →PCR clean up

Pecf11, Pecf20, crRBS, taRNA (1+2)+3,PCR product
1kbp Ladder, Pecf11(1+2+3), Pecf20(1+2+3), crRBS(1+2+3)

taRNA(1+2+3), Pecf11(all), Pecf20(all), crRBS(all), taRNA(all)

<img src="IMGP0068_20140811_3.JPG"/>

Making Plate

Ampicillin*20

preculture

A-6, B-10, C-7 #10

8,12,2014

Lab member

Nakashima,Nakamura,Yamanaka,Yoshikawa(Fresh),Yoshikawa


Contents

miniprep

A-6, B-10, C-6 #10 (made glycerol stock)

digestion →gel extraction

A-8 EX, C-6 ES, pSB1A2(A-1) XP *2 sample, A-9 linear XP
A-10 linear XP, A-7 linear XP, B-1 linear XP

1kbp Ladder, A-1 XP, A-1 XP, A-8 EX, C-6 ES
<img src="IMGP0070_20140812_1.JPG"/>
C-6:wrong
A-1:OK!

colony PCR

4-11L
<img src="IMGP0071_20140812_2.JPG"/>
OK!

ligation →TF

A-9, A-10, A-7, B-1

Making　reagent

LB 800ml

8,13,2014

Lab member

Yoshikawa,Nakashima,Tara,Nakamura,Takemura,Tsukada


Contents

miniprep

4-11L（made glycerol stock）

digestion→ gel extrction

A-8 EX, C-6 ES, A-6 SP, 4-11L XP

1kbp Ladder, A-6 SP, A-8 EX, C-6 ES, 4-11L XP
<img src="IMGP0072_20140813_1.JPG"/>
<img src="IMGP0073_20140813_2.JPG"/>
C-6:Incomplete cut
Backbone of 4-11L said to be pSB1A2, but ,actually,may be pSB1AK3.

ligation→TF

D-7 (4-11L XP + A-6 SP), D-7(C-6 ES + A-8 EX)

colony PCR

A-7 #1~2, A-9 #1~4, A-10 #1~4, B-1 #1~2 ,100bp Ladder
<img src="IMGP0074_20140813_3.JPG"/>
A-7 #1, A-10 #4：OK!

preculture

A-8, C-6, A-7 #1, A-10 #4

8,14,2014

Lab member

Yoshikawa,Nakashima,Tara,Takemura,Nakamura


Contents

miniprep

A-7 #1（made glycerol stock）, A-10 #4（made glycerol stock）, A-8, C-6

overlap extension PCR

Pecf 11, Pecf 20, crRBS, taRNA
(35 cycles, annealing in 59℃)

digestion → gel extranction

A-7 SP, 4-11L XP, pSB1A2 (B-10), A-7 linear XP
A-9 linear XP, A-10 linear XP, B-1 linear XP

1kbp Ladder, A-7 SP, 4-11L XP
<img src="IMGP0075_20140814_1.JPG"/>
<img src="IMGP0076_20140814_2.JPG"/>
OK!
A-10 XP, 100bp Ladder
<img src="IMGP0077_20140814_3.JPG"/>
OK!(low concentration)

100bp Ladder, pSB1A2(B-10), pSB1A2(B-10), A-7, A-9, B-1
<img src="IMGP0079_20140814_4.JPG"/>
<img src="IMGP0080_20140814_5.JPG"/>
Incomplete cut of pSB1A2 is weigh on our mind.

gel making

2% 25mL
1% 200mL

colony PCR

D-7(Ampicillin), D-7(Chloramphenicol)

1kbp Ladder, D-7(Amp) #1~4, D-7(CP) #1~4
<img src="IMGP0081_20140814_6.JPG"/>

ligation → TF

D-6 (A-7 SP + 4-11L XP), A-7 (linear XP + pSB1A2 XP)
A-9, A-10, B-1 (linear XP + pSB1A2 XP)

8,15,2014

Lab member

Yoshikawa,Nakashima,Tara,Takemura,Nakamura


Contents

miniprep

D-7 #3 (made glycerol stock)

digestion → gel extraction

A-10 SP, D-7 XP
<img src="IMGP0082_20140815_1.JPG"/>
<img src="IMGP0083_20140815_2.JPG"/>

colony PCR

A-7 #1~4, A-9 #1~4, A-10 #1~4, B-1 #1~4, D-6 #1~4
<img src="IMGP0085_20140815_3.JPG"/>
A-7 #3,4, A-10 #1,2,4, B-1 #1,2,4：OK!

D-6
<img src="IMGP0087_20140815_4.JPG"/>
OK!

ligation → TF

E-18 (A-10 SP + D-7 XP)

preculture

A-7 #3,4, A-10 #1,2,4, B-1 #1,2,4, D-6 #1

8,16,2014

Lab member

Yoshikawa


Contents

miniprep

A-7 #3,4, A-10 #1,2,4, B-1 #1,2,4, D-6 #1
(made glycerol stock)

8,18,2014

Lab member

Yoshikawa,Hiura


Contents

digestion → gel extraction

A-1 SP, A-2 SP, A-3 SP, D-6 XP *2, A-7 #3,4, B-10
<img src="IMGP0088_20140818_1.JPG"/>

sequence

A-7, A-7 #3,4, A-10, A-10 #1,2,4, B-1 #1,2,4
C-6 F, C-6 R, D-7 F, D-7 R, D-6 F, D-6 R

colony PCR

E-18 #1~3
<img src="IMGP0091_20140818_2.JPG"/>
OK!

ligation → TF

E-14 (A-3 SP + D-6 XP)
E-15 (A-1 SP + D-6 XP)
E-16 (A-2 SP + D-6 XP)

preculture

E-18 #1

8,19,2014

Lab member

Yoshikawa,Nakamura,Yamanaka,Yoshikawa(Fresh)


Contents

miniprep

E-18 (made glycerol stock)

digestion

A-8 XP, B-1 #1,2,4 SP
→Today, sequences of these dna proved to be wrong, so we disposed them.

making gel

2% gel:25ml

colony PCR

C-5, E-14, E-15,E-16

sequence

A-7:RBS
A-7 #3:none
A-7 #4:none
A-10:OK
B-1: all none

8,20,2014

Lab member

Yoshikawa,Nakamura,Tsukada,Yamanaka,Itoh


Contents

PCR

sigma11, sigma20, anti11, anti20

TF

1-21P

8,21,2014

Lab member

Yoshikawa,Nakashima


Contents

digestion → gel extraction

pSB1C3(A-4), A-6, B-3(We did not extract.), A-6'(NEB buffer)
<img src="IMGP0100_20140821_1.JPG"/>

colony PCR

A-2, A-3, A-4, 1-21P, NC
<img src="IMGP0101_20140821_2.JPG"/>

ligation → TF

B-3, C-2, C-2'

preculture

1-21P

8,22,2014

Lab member

Yoshikawa,Nakamura,Nakashima


Contents

miniprep

1-21P(made glycerol stock)

colony PCR

B-3, C-2, C-2
<img src="IMGP0102_20140822_1.JPG"/>

digestion → gel extraction

1-21P SP, D-7 XP, C-6 E, C-6 P, C-6(NC)
<img src="IMGP0103_20140822_2.JPG"/>
<img src="IMGP0104_20140822_3.JPG"/>

ligation → TF

K-1(1-21P SP + D-7 XP)

preculture

B-3 #1,4, D-7, C-2 #1,3, C-2' #3,4

8,23,2014

Lab member

Yoshikawa


Contents

miniprep

D-7, B-3 #1,4, C-2 #1,3, C-2' #3,4

8,25,2014

Lab member

Nakashima,Nakamura,Yamanaka,Hiura


Contents

digestion → gel extraction

A-8 EX *2, B-3 #1 ES, B-3 #4 ES, C-2 #1 ES, C-2 #3 ES
<img src="IMGP0105_20140825_1.JPG"/>
<img src="IMGP0106_20140825_2.JPG"/>

Plate　making

Ampicillin*10, Chloramphenicol*20

colony PCR

K-1
<img src="IMGP0107_20140825_3.JPG"/>

  1. 1,4:OK!


sequence

B-3 #1,4, C-2 #1,3, C-2' #3,4, E-18, A-2, A-3

ligation → TF

D-3 1 (A-8 EX + C-2 #1 ES), D-3 2(A-8 EX + C-2 #3 ES)
C-10 1(A-8 EX + B-3 #1 ES), C-10 2(A-8 EX + B-3 #4 ES)

preculture

A-2, A-8, K-1 #1, C-2 #1,3, B-3 #1

8,26,2014

Lab member

Yoshikawa,Nakashima,Yamanaka,Takemura,Tsukada


Contents

miniprep

K-1(made glycerol stock), A-2, A-8, B-3 #1, C-2 #1, C-2 #3

colony PCR

D-3 (1), D-3 (2), C-10 (1), C-10 (2)
<img src="IMGP0109_20140826_1.JPG"/>
OK!(C-10(1)#4 may be a little shorter.)

digestion → gel extraction

K-2 linear EP, K-3 linear EP, K-4 limear EP, K-5 linear EP, pSB1C3 (A-4) EP
<img src="IMGP0108_20140826_2.JPG"/>
We forgot to take the photo before we sliced the band.

ligation → TF

K-2, K-3, K-4, K-5

preculture

D-3 #1, C-10 #1

sequence

A-2:E-X-S-S-Pconst(weak)-S-P wrong

sequence

A-3:OK
B-3 #1:OK
B-3 #4:OK
C-2 #1:OK
C-2 #3:OK
C-2' #3:point mutation *2
C-2' #4:OK
E-18:OK

8,27,2014

Lab member

Yoshikawa,Nakashima,Yoshikawa(fresh),Itoh,Tara,Nakamura


Contents

miniprep

D-3, C-10(made glycerol stock)

digestion → gel extraction

A-1 SP, A-3 SP, A-10 SP, D-3 XP *2
<img src="IMGP0115_20140827_1.JPG"/>
<img src="IMGP0117_20140827_2.JPG"/>
D-3 is a little strange.Contamination?

colony PCR

K-2~5 #1~4

  • 100bp Ladder

<img src="IMGP0118_20140827_3.JPG"/>
500bp band:self ligation of backbone(A-4 200bop)
300bp band:If this band is blank vector, this band(EP cut) must be shorter than 300bp.

So, this band is OK!.

→K-2 #4, K-3 #1, K-4 #1,2, K-5 #2,3,4:OK!

ligation → TF

E-5(A-3 SP + D-3 XP), E-6(A-1 SP + D-3 XP), E-20(A-10 SP + D-3 XP)

preculture

E-15
D-3, C-10(Recovery)
K-2 #4, K-3 #1, K-5 #2

Remarks

<img src="IMGP0114_20140827_4.JPG"/>
We observed that pCMV expressed in Escherichia.coli.
Left:pCMV-GFP
Right:pConst(strong)-GFP
We may be able to carry out the characterization of pCMV and meet the gold medal requirement(parts implovement).

8,28,2014

Lab member

Yoshikawa,Nakashima,Itoh,Tara,Tsukada,Yamanaka


Contents

miniprep

K-2~5, E-23(made glycerol stock)
C-10, D-3

digestion →gel extraction

K-2 EX, K-3 EX, K-4 EX, K-5 EX, E-23 EP, C-6 ES *2, pSB1C3(A-4) EP, pSB1C3(A-4) XP
<img src="IMGP0119_20140828_1.JPG"/>
<img src="IMGP0120_20140828_2.JPG"/>
→pSB1C3 XP:keep in freezer

overlap extension PCR

A-7, A-9, B-1, B-2, D-8, D-9

check & colony PCR

check:E-5, E-6
colony PCR:(E-5 #1~4, E-6 #1~4, NC), A-7, A-9, D-8, B-1, B-2, D-9
<img src="IMGP0121_20140828_3.JPG"/>
A-7, A-9, B-1, D-8:OK!
B-2, D-9:Needs retry in higher concentration.
E-5 #2~4, E-6:OK!

ligation → TF

E-23'(E-23 EP + pSB1C3 EP), L-1~4(C-6 ES + K-2~5 EX)

8,29,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Tara,Tsukada,Yamanaka


Contents

miniprep

E-5(made glycerol stock)
A-4, C-6
E-6:No colony

digestion →gel extraction

A-7 linear XP, A-9 linear XP, A-10 SP, B-1 linear XP, D-3 XP, D-8 linear XP, E-5 SP, E-18 XP
<img src="IMGP0122_20140829_1.JPG"/>
<img src="IMGP0123_20140829_2.JPG"/>

PCR

B-2, D-9(retry)

check & colony PCR

E-23' #1~4, B-2, D-9
<img src="IMGP0124_20140829_3.JPG"/>
L-1~4
<img src="IMGP0125_20140829_4.JPG"/>
E-23 #1, L-1 #2,4, L-2 #4, L-3 #1~3, L-4 #2,4:OK!
PCR:failed

sequence order

E-23, K-1, K-2~5

ligation → TF

A-7, A-9, B-1, D-8( linear XP + pSB1C3 XP)
F-2 (E-18 XP + E-5 SP), E-20(A-10 SP + D-3 XP)

preculture

A-10, E-6, E-18, E-23', K-3, L-1~4

8,30,2014

Lab member

Yoshikawa


Contents

miniprep

A-10, E-18, K-3
L-1~4, E-23', E-6, E-18(made glycerol stock)

PCR

B-2, D-9

9,1,2014

Lab member

Nakashima,Itoh,Tara,Tsukada,Takemura


Contents

PCR clean up

B-2, D-9
<img src="IMGP0126_20140901_1.JPG"/>
failed

digestion → gel extraction

L-4 ES, E-6 ES, E-18 EX, L-1~3 ES, K-2~5 EX

<img src="IMGP0127_20140901_2.JPG"/>
<img src="IMGP0129_20140901_3.JPG"/>

Making plate

CP *30

colony PCR

A-7 #1~4, A-9 #1
<img src="IMGP0130_20140901_4.JPG"/>
OK!
D-8 #1~4, E-20 #1~4, F-2 #1~4 (100bp Ladder)
<img src="IMGP0131_20140901_5.JPG"/>
D-8 #1~4, E-20 #1~4,F-2#1,4:OK!
B-1 #1~4
<img src="IMGP0132_20140901_6.JPG"/>
OK!

Ligation

G-5 (E-6 ES + E-18 EX), M-1~4(K-2~5 EX + L-1~4 ES)

preculture

A-7 #1~4, A-9 #1, B-1 #1~4, D-8 #1~4, F-2, E-20

9,2,2014

Lab member

Yoshikawa,Nakashima,Hiura,Tara,Tsukada,Takemura


Contents

miniprep

A-7 #1~4, A-9, B-1 #1~4, D-8 #1~4, F-2, E-2(made glycerol stock)

digestion → gel extraction

A-4 SP, A-7 SP #1, A-7 SP #2, A-9 SP
<img src="IMGP0133_20140902_1.JPG"/>
<img src="IMGP0134_20140902_2.JPG"/>
A-8XP
<img src="IMGP0135_20140902_3.JPG"/>
<img src="IMGP0136_20140902_4.JPG"/>

B-1 #1, B-1 #2, B-3 XP, B-10 XP, D-7 XP, D-8 XP, E-20 XP, F-2 SP
<img src="IMGP0137_20140902_5.JPG"/>
<img src="IMGP0138_20140902_6.JPG"/>
D-8:???

sequence

A-7 #1~4, B-1 #1~4
D-8 #1~4
A-9, A-4, E-5, E-6, E-20

PCR

B-2, D-9

check & colony PCR

M-1~3 #1~4
<img src="IMGP0145_20140902_7.JPG"/>
OK!
M-4 #1~4, B-2, D-9, PC(VF2→B-10←VR )
NC, G-5
<img src="IMGP0146_20140902_8.JPG"/>
M-4 #1~4,PC(VF2→B-10←VR ):OK!
(M-4 #2~4 is a little longer?)

ligation → TF

C-4(A-7 SP + B-3 XP), C-5(A-7 SP + B-10 XP), E-17(A-9 SP + D-7 XP)

D-1(B-1 SP + D-8 XP), E-21(A-4 SP + D-8 XP), J-4 (F-2 SP + E-20 XP)


preculture

M-1~4 #1, G-5 #1, E-20

We got a result!!

simpler version of assay 1.
Left:Pσ11→GFP generator
Right:Pconst(strong)→σ11 generator & Pσ11→GFP generator
<img src="IMGP0144_20140902_9.JPG"/>

9,3,2014

Lab member

Yoshikawa,Nakashima,Hiura,Tara,Yamanaka,Takemura


Contents

M-1~4, G-5(made glycerol stock of M-3,4)

sequence

A-4: OK
A-7: all OK
A-9: OK
B-1:all OK
D-8#1: NG (2 skip)

    #2; OK

    #3: NG (1 mut.)

    #4; NG (2 mut. 2 skip)

E-5: OK
E-6: OK
E-20:OK

digestion → gel extraction

A-7 #1, 2 SP, C-9 XP, C-10 XP, K-2~4 EX, M-1~4 ES
<img src="IMGP0147_20140903_1.JPG"/>
<img src="IMGP0148_20140903_2.JPG"/>

colony PCR

C-4 a, C-5 a, D-1 a, E-17
<img src="IMGP0149_20140903_3.JPG"/>
C-4 a#2,3, C-5 a, D-1 a, E-17:OK!
E-21 b, J-4
<img src="IMGP0150_20140903_4.JPG"/>
J-4:wrong
E-21:a little shorter

PCR

B-2, D-9, B-7, B-2(Taq), D-9(Taq) Taq:positive control
anealing temperature:51℃～61℃(gradient)

ligation → TF

D-5 (A-7 SP + C-10 XP), D-6 (A-7 SP + C-9 XP), N-1~4 (M-1~4 ES + K-2~5 EX)

N-5 (M-1 ES K-3 EX), N-6 (M-3 ES + K-5 EX)

9,4,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Tara,Yamanaka,Tsukada


Contents

miniprep

E-20, D-1(made glycerol stock)

digestion → gel extraction

pSB1C3 (A-4) XS, pSB1C3 (A-4) XP, A-3 SP, D-1 XP
<img src="IMGP0154_20140904_1.JPG"/>
<img src="IMGP0155_20140904_2.JPG"/>

PCR check & clean up

D-9 #1,3,5,7,9
<img src="IMGP0151_20140904_3.JPG"/>
There are bands in #5,7,9
B-2 1,3,5,7,9,11, B-7.
<img src="IMGP0152_20140904_4.JPG"/>
There are bands in B-2 #5,7, B-7.
B-2 4,6, D-9 10,11,12
<img src="IMGP0153_20140904_5.JPG"/>
OK!
We decided to clean up B-2 #5, D-9 #11, B-7.

Making Plate

CP *30

digestion

B-2 linear SP, D-9 linear XP, B-7 linear XP

colony PCR

D-5, D-6, N-1, N-2 #1~4
<img src="IMGP0156_20140904_6.JPG"/>
D-5, D-6, N-1, N-2 #1,2:OK!
N-3~6 #1~4
<img src="IMGP0157_20140904_7.JPG"/>
N-3,N-4#1,3,4,N-5#1,4,N-6:OK!

ligation → TF

E-1 (A-3 SP + D-1 XP), B-2 (B-2 linear XP + pSB1C3 XP), D-9 (D-9 linear XP + pSB1C3 XP)

B-7 (B-7 linear XP + pSB1C3 XP), Emp. (pSB1C3 XS)


miniprep

M-1, M-2, C-4, C-5, E-17, E-21, J-4 (made glycerol stock)
K-2, K-5, C-9

preculture

F-2, D-5, D-6, N-1~6

9,5,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Takemura,Yamanaka


Contents

miniprep

F-2
N-1~6, D-5, D-6
→N-1, 2, 4, 5. 6 :failed

digestion → gel extraction

A-1 SP, A-3 SP, D-5 XP, D-6 SP, E-20 XP, K-4 EX, N-3 ES, F-2 SP
J-4 E(check)
<img src="IMGP0158_20140905_1.JPG"/>
<img src="IMGP0159_20140905_2.JPG"/>

ligation → TF

E-11 (A-3 SP + D-5 XP), E-12 (A-1 SP + D-5 XP), E-14 (A-3 SP + D-6 SP)

E-15 (A-1 SP + D-6 XP), O-3 (K-4 EX + N-3 ES)


PCR

B-2, D-9, B-7

colony PCR

B-2, B-7, D-9, E-1　#1~4
<img src="IMGP0160_20140905_3.JPG"/>
B-7 #2,3, D-9 #1, E-1 #3,4:OK!
Z-1 #2~4
<img src="IMGP0161_20140905_4.JPG"/>

preculture

B-7 #2,3, D-9 #1, E-1 #3, Z-1 #2, K-3, N-1,2,4~6

9,6,2014

Lab member

<p>Yoshikawa,Nakashima


</p>

Contents

miniprep

B-7 #2,3, D-9 #1, E-1 #3, Z-1 #2 (made glycerol stock)
N-1,2,4~6, K-3

PCR

B-2, D-9, B-7

check & colony PCR

B-2, D-9, B-7, B-2#5~8, B-7 #2,3,5,6, D-9 #5,6.7,8
<img src="IMGP0162_20140906_1.JPG"/>

9,8,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Takemura,Yamanaka,Tara


Contents

digestion → gel extaction

B-2 linear XP (did not extracted)
N-1,2,4~6 ES
<img src="IMGP0163_20140908_1.JPG"/>
<img src="IMGP0165_20140908_2.JPG"/>
A-4 SP, pSB1C3 (A-4) XP, A-6 SP, B-7 #2 XP, B-7 #3 XP, D-9 #1 XP, K-2,3,5 EX
<img src="IMGP0166_20140908_3.JPG"/>
B-7 #2, D-9 #1:disposed(incomplete cut)
B-7 #3:disposed(a little longer)(This band proved to be correct.9/9 wrote)
<img src="IMGP0167_20140908_4.JPG"/>

colony PCR

E-11,12,14,15 #1~4
<img src="IMGP0168_20140908_5.JPG"/>
OK!
O-3 #1,2
<img src="IMGP0169_20140908_6.JPG"/>

  1. 2:OK!


PCR

B-2
<img src="IMGP0170_20140908_7.JPG"/>
B-7,D-9
<img src="IMGP0171_20140908_8.JPG"/>
The band of the longest DNA is OK!

ligation → TF

O-1 (N-1 ES + K-2 EX), O-2 (N-2 ES + K-3 EX), O-4 (N-4 ES + K-5 EX), O-5 (N-5 ES + K-3 EX)
O-6 (N-6 ES + K-5 EX), B-2 (B-2 linear XP (9/8 PCR) + pSB1C3 XP)
B-2' (B-2 linear XP + pSB1C3 XP), D-9, B-7

preculture

D-5, D-6, N-3, E-21, A-3, K-4
E-11 #1, E-12 #1, E-14 #1, E-15 #1, O-3 #2, B-7 #6, D-9 #5,6

9,9,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Takemura,Yamanaka


Contents

miniprep

E-11,12,14,15, O-3, B-7 #6, D-9 #5,6 (made glycerol stock)
D-5, N-3, K-4, A-3
D-6:did not grow
D-5:diposed(doubt of cross contamination)

digestion → gel extraction

B-2 linear XP (did not extracted)
pSB1C3 (A-4) XP, A-6 SP, A-8 EX, B-7 #6 XP, D-9 linear XP, B-7 linear , E-1 EX, E-14 ES, E-15 ES, O-3 ES
<img src="IMGP0172_20140909_1.JPG"/>
<img src="IMGP0173_20140909_2.JPG"/>

colony PCR

B-2 #1~3, B-2' #1~4, B-7 #1~3, D-9 #1,2, O-1 #1~4
<img src="IMGP0175_20140909_3.JPG"/>
B-2' #1, B-7 #1,2, O-1:OK!
O-2,4,5,6 #1~4
<img src="IMGP0176_20140909_4.JPG"/>
O-2,3,4,6,O-5#2,3,4

sequence

B-7 #2: NG
B-7 #3: OK
D-9 #1: NG
E-1: OK
C-4: OK
C-5: OK
D-5: OK
D-6: OK
E-17: OK
E-21: OK
F-2: ?
J-4: OK
N-1: OK
N-2: M-2
N-3: OK
N-4: M-4
N-5: OK
N-6: OK

ligation → TF

D-9 (D-9 linear XP + pSB1C3 XP) *2, B-2 (B-2 linear XP + pSB1C3 XP)*2

P-3 (O-3 ES + A-8 EX), I-1 (E-14 ES + E-1 EX), I-2 (E-15 ES + E-1 EX)


preculture

F-2, D-5,6, D-9 #5,6, E-12, K-4
O-1,2,4,6 #1, O-5 #2, B-2' #1

9,10,2014

Lab member

Nakashima,Hiura,Takemura,Yamanaka,Yoshikawa(Fresh)


Contents

miniprep

O-1,5,6, N-2,4, F-2, B-2' #1 (made glycerol stock)
E-12, K-4, D-5
D-6:did not grow

digestion → gel extraction

A-6 SP, A-7 SP, A-8 EX, B-2' XP, B-7 XP
<img src="IMGP0177_20140910_1.JPG"/>
<img src="IMGP0179_20140910_2.JPG"/>
K-3 EX, K-5 EX, O-1 ES, N-2 ES, N-4 ES
<img src="IMGP0180_20140910_3.JPG"/>
<img src="IMGP0181_20140910_4.JPG"/>
O-5 ES, O-6 ES
<img src="IMGP0182_20140910_5.JPG"/>
<img src="IMGP0183_20140910_6.JPG"/>

colony PCR

I-1 #1~4
<img src="IMGP0185_20140910_7.JPG"/>
B-2(140909) #1~8, D-9 #1~8
<img src="IMGP0184_20140910_8.JPG"/>
B-2 #2, D-9 all, I-1 #2~4, I-2 #1~4:OK!

ligation → TF

O-2 (N-2 ES + K-3 EX), O-4 (N-4 ES + K-5 EX), P-1 (O-1 ES + A-8 EX), P-5 (O-5 ES + A-8 EX)

P-6 (O-6 ES + A-8 EX), C-1' (B-2' XP + A-6 SP), C-3' (B-2 ' XP + A-7 SP), C-8 (B-7 XP + A-6 SP)


preculture

D-9 #5,6, D-9(140909) #1~4, B-2 (140909) #2, I-1 #2, I-2 #1, D-6 #1

9,11,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Hiura,Itoh,Tsukada


Contents

miniprep

D-9 #1~4, B-2 #2, I-1, I-2, D-6 (made glycerol stock)

digestion → gel extraction

A-1 EP, pSB1C3(A-3) EP *2, A-4 SP, A-6 SP, A-7 SP, A-8 EX, A-10 EX, B-2 #2 XP, E-6 EP, E-12 EP
<img src="IMGP0186_20140911_1.JPG"/>
<img src="IMGP0187_20140911_2.JPG"/>
E-15 EP, E-18 EP, E-20 EP, D-9 #1 XP, O-3 ES
<img src="IMGP0188_20140911_3.JPG"/>
<img src="IMGP0189_20140911_4.JPG"/>

sequence

B-2' #1, B-2 #2, F-2, E-11, E-12, E-14, E-15, O-1, N-2, O-3, N-4, O-5, O-6, D-9 #1~4

Plate Making

CP *30

colony PCR

C-1', C-3', C-8, O-2, O-4 #1~3
<img src="IMGP0190_20140911_5.JPG"/>
C-1', C-3', C-8, O-2, O-4 #1,2:OK!
P-1, P-5, P-6 #1~3
<img src="IMGP0192_20140911_6.JPG"/>
→P-1 #1~3, P-5 #2, P-6 #1~3:OK!

ligation → TF

C-1 (A-6 SP + B-2 #2 XP), C-3 (A-7 SP + B-2 #2 XP)

E-22a (A-4 SP + D-9 #1 XP), E-22b (A-4 SP + D-9 #2 XP), P-3 (O-3 ES + A-8 EX)

A-10 CP, A-1 CP, E-6 CP, E-12 CP, E-20 CP, E-15 CP (replace backbone to pSB1C3)

preculture

C-1' #1, C-3' #1, C-8 #1, O-2 #1, O-4 #1, P-1 #1, P-5 #2, P-6 #1, E-12,14,15,18, A-1, O-1,3,5

9,12,2014

Lab member

Yoshikawa,Nakashima,Hiura,Itoh,Tsukada,Tara


Contents

miniprep

C-1', C-3', O-2, O-4, P-1, P-5, P-6 (made glycerol stock)
E-12, E-14, E-15, O-1, O-3, O-5, E-18, A-1

digestion → gel extraction

A-6 SP, A-8 EX *2, B-2 XP, C-1' ES, C-3' ES, C-8 ES, K-6 SP, O-2 ES, O-3 ES, O-4 ES
<img src="IMGP0193_20140912_1.JPG"/>
<img src="IMGP0194_20140912_2.JPG"/>
C-1,3:did not extracted
P-1 XP, P-5 XP, P-6 XP
<img src="IMGP0195_20140912_3.JPG"/>
<img src="IMGP0196_20140912_4.JPG"/>

colony PCR

A-1 CP, A-10 CP, C-3, E-6 CP #1~3
<img src="IMGP0198_20140912_5.JPG"/>
E-12 CP, E-15 CP, E-20 CP, E-22a, E-22b
<img src="IMGP0199_20140912_6.JPG"/>
except E-20#1,2:OK!

sequence

B-2' #1: NG
B-2 #2: OK
D-9 #1: NG

   #2: NG

   #3: NG

   #4: OK

O-1: OK
O-3: OK
O-5: OK
O-6: OK
N-2: OK
N-4: OK
E-11: Ok
E-12: OK
E-14: OK
E-15: OK
F-2: OK

ligation → TF

C-1 (A-6 SP + B-2 XP), P-2~4 (O-2~4 ES + A-8 EX), Q-1,5,6 (P-1,5,6 XP + K-6 SP)

ligation

P-3 (O-3 ES + A-8 EX)

preculture

A-4, A-6, A-8, C-3 #1, A-1 CP #1, E-12 CP #1, E-15 CP #1, E-20 CP #3, E-6 CP #1

9,13,2014

Lab member

Yoshikawa


Contents

miniprep

A-4, A-6, A-8 C-3, E-6 CP, E-12 CP, E-15 CP, E-20 CP, A-1 CP(made glycerol stock)

9,15,2014

Lab member

Yoshikawa,Nakashima,Takemura,Nakamura,Yamanaka,Tara


Contents

digestion → gel extraction

A-8 EX, C-3 ES
<img src="IMGP0200_20140915_1.JPG"/>
<img src="IMGP0201_20140915_2.JPG"/>

colony PCR

C-1 (σ20F, VR)
<img src="IMGP0202_20140915_3.JPG"/>
OK!

ligation → TF

D-4 (C-3 ES + A-8 EX) *2 (new and old competent cell)

preculture

C-1 #1

9,16,2014

Lab member

Yoshikawa,Nakashima,Hiura,Itoh,Nakamura,Yamanaka


Contents

miniprep

C-1　(made glycerol stock)

colony PCR

D-4, P-2~4
<img src="IMGP0206_20140916_1.JPG"/>
A-10
<img src="IMGP0207_20140916_2.JPG"/>

Q-1,5,6:confirmed by fluorescence

digestion → gel extraction

A-4 SP, A-8 EX, C-1 ES, D-9 XP, E-18 EP, pSB1C3 (A-4) EP
<img src="IMGP0205_20140916_3.JPG"/>

ligation → TF

D-2 (A-8 EX + C-1 ES), E-18 CP (E-18 EP + pSB1C3 EP) *2, E-22 (A-4 SP + D-9 XP)

preculture

D-4, P-2~4, A-10 CP, Q-1,5,6

assay

<img src="IMGP0204_20140916_4.JPG"/>
PC (E-23': Pconst (strong)-GFP-d.term)
Experiment (K-1: pCMV-GFP-d.term)
NC (Z-1: pSB1C3)
absorbance:600nm and 395nm Absorbance of 395nm proved not to be able to measure.
We need fluorospectro-photometer.

9,17,2014

Lab member

Yoshikawa,Nakashima,Hiura,Takemura,Yoshikawa(Fresh),Yamanaka


Contents

miniprep

D-4, P-2~4, Q-1,5,6, A-10 CP　(made glycerol stock)

digestion → gel extraction

A-1 CP SP, A-3 SP, D-4 XP, K-6 SP, P-2 XP, P-3 XP, P-4 XP
<img src="IMGP0208_20140917_1.JPG"/>
<img src="IMGP0209_20140917_2.JPG"/>

colony PCR

D-2, E-18 CP, E-22
<img src="IMGP0218_20140917_3.JPG"/>

ligation → TF

E-8 *2 (A-3 SP + D-4 XP), E-9 (A-1 CP SP + D-4 XP), Q-2~4 (K-6 SP + P-2~4 XP)

assay

Photo of plate(n=4)
<img src="IMGP0211_20140917_4.JPG"/>
<img src="IMGP0210_20140917_5.JPG"/>
<img src="IMGP0213_20140917_6.JPG"/>
<img src="IMGP0212_20140917_7.JPG"/>
<img src="IMGP0215_20140917_8.JPG"/>
<img src="IMGP0214_20140917_9.JPG"/>
<img src="IMGP0217_20140917_10.JPG"/>
<img src="IMGP0216_20140917_11.JPG"/>

9,18,2014

Lab member

Yoshikawa,Nakashima,Hiura,Tsukada,Nakamura


Contents

miniprep

D-2, E-18 CP, E-22
(made glycerol stock)

digestion → gel extraction

A-1 CP SP, A-3 SP, A-9 SP, D-2 XP, E-22 SP, G-5 XP
<img src="IMGP0219_20140918_1.JPG"/>
<img src="IMGP0220_20140918_2.JPG"/>

colony PCR

E-8, E-9　#1~4
<img src="IMGP0221_20140918_3.JPG"/>
Q-2~4 :confirmed by fluorescent

ligation → TF

E-2 (A-3 SP + D-2 XP)
E-3 (A-1CP SP + D-2 XP)
E-19 (A-9 SP + D-2 XP)
H-5 (E-22 SP + G-5 XP)

preculture

E-8,9, Q-2~4

9,19,2014

Lab member

Yoshikawa,Nakashima,Hiura,Tara,Yamanaka,Nakamura


Contents

miniprep

E-8,9, Q-2~4

digestion → gel extraction

A-9 SP, D-2 XP, E-22 XP, F-2 SP, G-5 SP
<img src="IMGP0222_20140919_1.JPG"/>
<img src="IMGP0223_20140919_2.JPG"/>

colony PCR

E-2, E-19 #1~4
E-3, H-5 :No colony
<img src="IMGP0225_20140919_3.JPG"/>

ligation → TF

H-5 (G-5 SP + E-22 XP)
H-4 (F-2 SP + E-22 XP)

preculture

E-2 E#1, E-19 #1

9,20,2014

Lab member

Yoshikawa


Contents

miniprep

E-2, E-19

9,22,2014

Lab member

Yoshikawa,Nakashima,Takemura,Nakamura,Tara,Yamanaka


Contents

digestion → gel extraction

A-1 SP, D-2 XP, E-2 ES, E-17 EX, E-22 XP, G-5 SP, J-4 SP
<img src="IMGP0226_20140922_1.JPG"/>
<img src="IMGP0235_20140922_2.JPG"/>

colony PCR

H-4
<img src="IMGP0236_20140922_3.JPG"/>
H-4#1,2,3:OK!

ligation - TF

H-5 (G-5 SP + E-22 XP)
J-6 (J-4 SP + E-22 XP)
E-3 (A-1 SP + D-2 XP)
F-1 (E-2 ES + E-17 EX)

9,23,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Tara,Yamanaka

Contents

digestion, gel extraction

A-1CP SP, D-2 XP, E-22 XP, G-5 SP
J-4 SP(stopped)
<img src="IMGP0241%27.jpg"/>
<img src="IMGP0242%27.jpg"/>

miniprep

H-4(made glycerol stock)

colony PCR

J-6
<img src="IMGP0243%27%27%27.jpg"/>
OK!

ligation,TF

H-5(G-5 SP+E22 XP)
E-3(A-1 SP+D-2 XP)

9,24,2014

Lab member

Nakashima,Yamanaka,Takemura


Contents

miniprep

F-1,J-6(made glycerol stock)
E-22,D-6
G-6 did not grow.
We disposed J-6.(dropped on the floor)

digestion,gel extraction

A-1 SP,E-2 ES,E-5 ES,E-17 EX,E-18CP EX,E-21 XP,G-5 SP,D-2 XP,F-1 SP,E-19 XP,E-22 XP
<img src="IMGP0244%27.jpg"/>
<img src="IMGP0245%27.jpg"/>

ligation,TF

J-2(F-1 SP+E-1 XP)
H-1(F-1 SP+E-21 XP)
F-3(E-5 ES+E-17 EX)
F-4(E-2 ES+E-18 EX)
E-3(A-1 SP+D-2 XP)
H-5(G-5 SP+E-22 XP)

9,25,2014

Lab member

Nakashima,Nakamura,Tara,Tsukada,Yamanaka


Contents

miniprep

A-1CP, J-6

digestion,gel extraction

A-1 SP,D-2 XP,E-5 ES,E-17 EX,E-19 XP,E-21 XP,E-22 XP,F-1 SP,G-5 SP
<img src="IMGP0247%27.jpg"/>
<img src="IMGP0248%27.jpg"/>

ligation,TF

J-2(F-1 SP+E-19 XP)
H-1(F-1 SP+E-21 XP)
F-3(E-5 ES+E-17 EX)
E-3(A-1 SP+D-2 XP)
H-5(G-5 SP+E-22 XP)

Making competent cell


preculture

G-5,E-5,E-17,E. coli JM109

competent cell(made at 0911) check

Ampicillin,Chloramphenicol,non-antibiotics
culture in LB 3ml
LB midium 3ml, as N.C.
culture for 2h.
no TF

result of check

Ampicilln,non-antibiotics:become clouded
Chroramphenicol,LB:did not grow
So,we confirmed ampicillin resistant plasmid exists in competent cell and we disposed it.

9,26,2014

Lab member

Yoshikawa,Nakashima,Tara,Yoshikawa(Fresh),Tsukada


Contents

miniprep

G-5,E-5,E-17

digestion

A-1CP SP,D-2 XP,G-5 EP(strange band appeared,disposed),pSB1C3(A-4) EP,A-1 SP
<img src="IMGP0249%27%27.jpg"/>
<img src="IMGP0250%27.jpg"/>

colony PCR

F-3,F-4,H-1,H-5,J-2
<img src="IMGP0251%27.jpg"/>
<img src="IMGP0253%27.jpg"/>
OK!

ligation,TF

E-3(D-2 XP+A-1SP)
E-3CP(D-2 XP+A-1CP SP)

preculture

F-3,F-4,H-1,H-5,J-2#1,G-5

9,27,2014

Lab member

Yoshikawa,Nakashima

Contents

TF

E-3(chemical)

miniprep

F-3,F-4,H-1,H-5,J-2(made glycerol stock)
G-5

TF

E-3CP(electroporation)

9,29,2014

Lab member

Yoshikawa,Nakashima,Nakamura,Tara,Takemura


Contents

digestion,gel extraction

A-1CP SP,pSB1C3(A-4) EP,D-2 XP,E-21 XP,G-5 EP,H-5 EP,J-2 SP
<img src="IMGP0256%280929%29%27.jpg"/>
<img src="IMGP0257%27.jpg"/>

Gel making


ligation

G-5CP(G-5 EP+pSB1C3 EP)
H-5CP(H-5 EP+pSB1C3 EP)
J-5(J-2 SP+E-21 XP)
E-3(A-1CP SP+P-2 XP)

preculture

E-5,E-11,E-22
K-1,E-23',Z-1(for assay)

PCR

VF2-(ligation product of E-3)-VR E-3 lig:1uL
VF2:1uL
VR:1uL
Taq:5uL
MilliQ:2uL
Total:10uL
extention:1m12sec
<img src="IMGP0258%280929%29%27.jpg"/>
We observed the band which had expected length.
The ligtion must be OK!

9,30,2014

Lab member

Yoshikawa.Nakamura,Tsukada,Tara,Yamanaka


Contents

miniprep

E-2,E-5,E-11

digestion,gel extraction

We could not find D-2 sample(probably accidentally disposed).
So we stopped it.

colony PCR

G-5,H-5,J-5
<img src="IMGP0258%280930%29%27.jpg"/>
H-5,J-5#1,2,3,4:OK!

Making M9 medium

1M MgSO4 50ml
1M CaCl2 10ml
20% glucose 50ml

digestion(cutcheck)

G-5 E,G-5 P,G-5 non-cut
<img src="IMGP0260%27.jpg"/>
Strange bands appeared.
We decided to read a sequence of this part.

10,1,2014

Lab member

Nakashima,Tara,Nakamura,Takemura


Contens


miniprep

H-5CP,G-5CP,J-5(made glycerol stock)

sequence order

E-2,E-8,E-9,E-19,F-1,F-3,F-4,G-5,H-1,H-4
H-5(VF-VR,Psigma2F-anti2R),J-2,J-4,J-5,J-6

10,2,2014

Lab member

Nakashima,Nakamura,Tara


Contents

assay

completely failed

In M9 medium, Escherichia.coli JM109 did not grow well.(doubling time:1h)
The glycerol stock needed to be put a lot.

culture

F-2,F-3,F-4(for M9 check)

PCR

G-1(F,R),G-4(F,R),G-5(F,R),H-1(F,R),H-4(F,R),H-5(F,R)
Template:1ng/uL,1uL

Making M9 medium

5*M9 24mL
20% Glu 1.2mL
MgSO4 240uL
CaCl2 12uL
Amino acid 10mL
mess up to 1L

PCR check

<img src="IMGP0261%27.jpg"/>

OK!(except G-5)

PCR

G-1,G-4,H-1,H-4
extension time:6m30sec
EpCAM nested
95C 3min-(-95C 30sec-48C 30sec-72C 3min-)*30-72C 5min-4C

preculture

E-17,E-18,F-1,F-2,F-3,F-4,Z-1(M9)
E-23'(LB)

10,3,2014

Lab member

Nakashima,Tara,Nakamura


Contents

PCR clean up and check

<img src="IMGP0262%27.jpg"/>
G-1,G-4,H-1,H-4:Band in correct position and unexpected position.
EpCAM:No band

digestion,gel extraction

<img src="IMGP0263%27.jpg"/>
<img src="IMGP0264%27.jpg"/>
G-1deg linear EP,G-4deglinear EP,H-1deg linear EP,H-4deg linear EP(A-4)
(deg:degradation tag)

PCR

EpCAM nested
94C 3min-(-94C 30sec-48C 30sec-72C 3min-)*30-72C 5min-4C <img src="IMGP0265%27.jpg"/>
No band

gel making


ligation,TF

H-1'(H-1'linear EP+pSB1C3 EP)
H-4'(H-4'linear EP+pSB1C3 EP)
G-1'(G-1'linear EP+pSB1C3 EP)
G-4'(G-4'linear EP+pSB1C3 EP)

PCR

EpCAM nested
95C 3min-(-95C 30sec-46C 30sec-72C 3min-)*30-72C 5min-4C <img src="IMGP0267%27.jpg"/>
No band

10,4,2014

Lab member

Yoshikawa

assay1

F-2 in 20mL culture
When OD600=0.516,we put 10% arabinose 20uL.
F-1 in 20mL culture
When OD600=0.514,we put 10% arabinose 20uL.
Z-1 in 20mL culture
When OD600=0.544,we put 10% arabinose 20uL.
O/N culture

Culture in 3mL:disposed(OD600 value did not agrees with each other.)

colony PCR

H-1deg,H-4deg,G-1eg,G-4deg
We confirmed by fluorescence.

preculture

H-1deg,H-4deg#1,G-1eg,G-4deg

10,5,2014

Lab member

Yoshikawa


Contents

miniprep

H-1deg,H-4deg#1,G-1eg,G-4deg

preculture
(M9)

E-15CP,E-17,E-18CP,I-2,F-1,2,3,4,Z-1

10,6,2014

Lab member

Yoshikawa,Nakashima,Tara


Contents

assay1,3

E-15,I-2,F-1,F-2,F-3,F-4,F-17,E-18,Z-1
Measured the OD 600 value.
Except I-2,F-2,the value is more than 1.0.
F-1,F-3,E-17,Z-1(20 fold dilution):Culture in 3mL. The composition of M9 proved to be wrong,so disposed.

Making M9 medium

5*M9 200mL
20% Glu 10mL
amino acids 10mL
1M MgSO4 2mL
1M CaCl2 100uL
MilliQ 778mL
Total 1000mL

result of OD600

F-3(non-Chloramphenicol)
1h:0.094
2h:0.086
F-3(Chloramphenicol) 1h:0.074
2h:0.073

assay1

(tentative)

We measured the fluorescence of F-1,F-2,Z-1(culture in 20mL).
Ex:501nm
F-2:We observed a peak in 511nm.
F-1,Z-1:No peak

preculture

F-1,F-2,F-3,F-4,E-15CP,I-2,Z-1,E-17,E-18CP(M9 medium)
E-23'(LB medium)

10,7,2014

Lab member

Yoshikawa,Nakashima,Tara,Nakamura


Contents

assay

F-1,F-13,E-17,Z-1,E-15,I-2
culture in M9
except E-15,OD600 is more than 1.0.
E-15:over 0.85
F-2:only 0.3
E-23':over 2.0,culture in LB medium
All samples was cultured in 3 mL.(n=5)
F-1,Z-1:cultured in 20mL(flask)(n=1)

OD600 before subculture

E-15:1.113
E-11:0.933
E-18:1.190
E-23':forgot to measure
F-1:0.927
F-2:0.374
F-3:0.878
F-4:0.992
I-2:0.888
Z-1:1.084

PCR

J-5(F,R),J-6(F,R)
J-5:OK!
extension time
F:1800+200=2000bp,4min
R:1850+200=2050bp,4min

PCR

J-6(F,R)
J-5R
extension time:1min

10,8,2014

Lab member

Yoshikawa,Nakashima,Tara


Contents

making gel


subculture

Escherichia.coliMG1655
100 fold dilution
20mL,flask

PCR clean up and check

<img src="IMGP0268%27.jpg"/>
<img src="IMGP0269%27.jpg"/>
J-6F:low concentration
J-6R:shorter band is OK!
We decided that we made J-5,J-6
as H5deg-positive feedback circuit, and H6deg-positive feedback circuit.

sequence order

G-1deg,G-4deg,H-1deg,H-4deg

TF

(electroporation)

F-1,F-2,F-3,F-4,E-17,E-18CP,Z-1,I-2,E-15
G-1deg,G-4deg,H-1deg,h-4deg,J-4,J-4
also,2mL culture as recovery.

10,9,2014

Lab member

Yoshiakwa,Nakashima,Tara

Contents

digestion,gel extraction

E-19 XP,E-20 XP,H-1deg SP,H-4deg SP

making gel


making plate

Chloramphenicol*10

ligation

J-5deg(E-19XP+H-1degSP)
J-6deg(E-20XP+H-4degSP)
We did not have competent cell of E.coli MG1655, so put it in freezer.

preculture

F-1,E-17,E-15,Z-1,I-2:both in LB medium and M9 medium.
MG1655:LB medium

10,10,2014

Lab member

Yoshikawa,Nakashima,Tara,Yamanaka

assay1,3

I-2,E-15,Z-1,F-1,E-17(made glycerol stock,subculture in 20 fold dilution)
MG1655 did not grow, because we accidentally put antibiotics.
OD600(O/N culture)
E-15:1.795
E-17:1.781
F-1:1.937
I-2:1.886
Z-1:1.780

making M9 medium
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