Team:UGA-Georgia/Notebook
From 2014.igem.org
(Difference between revisions)
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<li> Since this pre-study gave us consistent, positive values for fluorescence, we were able to now refine the oxygen exposure protocol and apply it to characterization of our parts. </li> | <li> Since this pre-study gave us consistent, positive values for fluorescence, we were able to now refine the oxygen exposure protocol and apply it to characterization of our parts. </li> | ||
<ul> | <ul> | ||
- | <li> This detailed protocol can be found in our <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">protocol</a> section </li> | + | <li> This detailed protocol can be found in our <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">protocol</a> section. </li> |
</ul> | </ul> | ||
+ | <li> Revival of frozen stocks of cells containing plasmids with our theoretical "perfect" RBS (14), cells containing our theoretical "negative" RBS (15), and each of the 3 colonies of 12. Also, revival of WT with no plasmid were revived from a room temperature stock (negative control). </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5">October</font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1</h5> | ||
+ | <li> The revived parent strains of 14, 15, WT, and 12 C-1,2,3 were inoculated into varying volumes, and in triplicates, to test for a linear relationship of mCherry production to volume of culture which would be determined after the samples were taken to the plate reader. </li> | ||
+ | <ul> | ||
+ | <li> The triplicates were grown in 5mL, 25mL, and one single sample each of 12 C-1, 14, and 15 were grown into 100mL as a reference. </li> | ||
+ | </ul> | ||
+ | |||
Revision as of 16:02, 17 October 2014
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