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Team:Oxford/InterlabResults
From 2014.igem.org
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<center>View our interlab results pdf <a href="https://static.igem.org/mediawiki/2014/8/86/Oxigem_Interlab_results.pdf" target="_blank">here!</a></center> | <center>View our interlab results pdf <a href="https://static.igem.org/mediawiki/2014/8/86/Oxigem_Interlab_results.pdf" target="_blank">here!</a></center> | ||
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| - | We have given each measurement here in arbitrary units - as provided by our laboratory's plate-reader and spectrophotometer.<br>Adjusted fluorescence is calculated as the mean fluo of each biological replicate minus the mean fluo for the DH5-a, giving an approximation for the fluorescence due to the devices only.<br><br><br>Cell counts were estimated based the OD, using the online calculator given by the link: www.genomics.agilent.com%2fbiocalculators%2fcalcODBacterial.jsp%3f_requestid%3d826255 <br><br><br> Results were given in triplicate with respect to biological replicates (meaning three separate colonies were taken from the same plates); and duplicate with respect to the technical replicates (two 200ul samples were taken from each culture to be read).<br>Although it is clear from visual inspection that there are significant differences in fluorescence depending on device, the fluorescence of each sample can be modelled as the following function:<br><br>FLUO = DEVICE + AUTO(CELLS) + AUTO(MEDIA) + ERROR(BIOLOGICAL) + ERROR(TECHNICAL). | + | We have given each measurement here in arbitrary units - as provided by our laboratory's plate-reader and spectrophotometer.<br><br><br>Adjusted fluorescence is calculated as the mean fluo of each biological replicate minus the mean fluo for the DH5-a, giving an approximation for the fluorescence due to the devices only.<br><br><br>Cell counts were estimated based the OD, using the online calculator given by the link: www.genomics.agilent.com%2fbiocalculators%2fcalcODBacterial.jsp%3f_requestid%3d826255 <br><br><br> Results were given in triplicate with respect to biological replicates (meaning three separate colonies were taken from the same plates); and duplicate with respect to the technical replicates (two 200ul samples were taken from each culture to be read).<br><br><br>Although it is clear from visual inspection that there are significant differences in fluorescence depending on device, the fluorescence of each sample can be modelled as the following function:<br><br><br>FLUO = DEVICE + AUTO(CELLS) + AUTO(MEDIA) + ERROR(BIOLOGICAL) + ERROR(TECHNICAL).</div> |
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Revision as of 15:26, 17 October 2014
