Team:Groningen/Template/MODULE/projects/parts/biobricktable

From 2014.igem.org

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Revision as of 14:21, 17 October 2014

iGEM Groningen 2014 assembled parts
 
EMPTYPartTypeDescriptionDesignerLength
EMPTYBBa_K1365000CodingnisANisin precursor. Should be used in combination with the nisin gene cluster (e.g. Lactococcus lactis NZ9800).Sandra Mous174
EMPTYBBa_K1365003CodingnisCProtein needed in the cyclization of the nisin precursor. Part of the nisin gene cluster.Sandra Mous1245
EMPTYBBa_K1365020compositenisRKTwo proteins involved in the recognition of nisin and positive feedback regulation to induce the pNisA promoter. This biobrick is part of the nisin gene cluster.Sandra Mous2023
EMPTYBBa_K1365020CodingsfGFPSuperfolder GFP is a more table protein than more communly used GFP. It gives an higher fluorescence as well1.Rick Elbert1625
EMPTYBBa_K1365100DeviceEMPTYDual action system against S. aureus. System made for L. lactis and codes for nisin precursor and the biofilm degradeding enzyme DspB. The latter is fused with an USP45sp for secretion from the cell. This device has an P2 operon and this thus transcriped after binding from p-AgrA.Rick Elbert1625
EMPTYBBa_K1365111CodingssUsp45-dspBDispersinB fused to an Usp45sp. The dspB enzyme is secreted from the cell via the usp system of L. lactis2.Rick Elbert1170
EMPTYBBa_K1365120DeviceEMPTYDual action system against P. aeruginosa. System is made for L. lactis and includes the genes for autoinducer inactivation enzyme A (AiiA) and Dispersin B (DspB).Rick Elbert2350
EMPTYBBa_K1365130DeviceEMPTYEMPTYRick ElbertEMPTY
EMPTYBBa_K1365169CodingssUsp45-aiiAAutoinducer inactivation enzyme A (AiiA) gene fused to signal sequence for the usp45 secretion pathway.Rick Elbert834
EMPTYBBa_K1365301PlasmidpIL2533Plasmid for L. lactis including the biobrick compatible VF2 and RV primer binding sites, prefix and suffix. No illegal restriction sites.Lisa Hielkema6230
EMPTYBBa_K1365555CompesitionRBS-sfGFP-dtCompesition of the superfolder GFP combined with RBS and double terminator (dt). This part was used to assemble with different promoters used for characterization experiments.Aakanksha Saraf874
EMPTYBBa_K1365556 CompesitionRBS-nisA-dtCompesition of the precursor nisA combined with an RBS and double terminator (dt). This part was assembled with a range of promotor to be used for characterization experiments.Aakanksha Saraf328
EMPTYBBa_K1365557ReporterEMPTYESuperfolder GFP under a constitituve promoter. Desingend for the use in L. lactis and E. coli.PTYAakanksha Saraf915
EMPTYBBa_K1365997DeviceEMPTYAHL sensor. The LasR membrane protein is produced by this device. Chandhuru Jagadeesan1003
EMPTYBBa_K1365998SignalingEMPTYAHL generator. This part can be used to mimic the presence of AHL producing bacteria P. aeruginosa.Chandhuru Jagadeesan892
EMPTYBBa_K1365999compesitionRBS-GFP-dtComposition of ribosome binding site, green fluorescence protein and double terminator. Brick is both L. lactis as E. coli compatible.Chandhuru Jagadeesan873
EMPTYEMPTYEMPTYEMPTYEMPTYEMPTYEMPTY