Team:INSA-Lyon/Results
From 2014.igem.org
(Difference between revisions)
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<p><b>Figure 3: Engineered bacteria biofilm characterization and quantification using Confocal Laser Scanning Microscopy</p></b> | <p><b>Figure 3: Engineered bacteria biofilm characterization and quantification using Confocal Laser Scanning Microscopy</p></b> | ||
- | <p>All the strains used are constitutively fluorescent to allow detection with confocal laser microscopy (ZEISS LSM510 META, 40X/1.3OILDIC, laser Argon 4 lines 30 W 458 nm, 477 nm, 488 nm, 514 nm, <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">See Protocole</a>). Positive control/CsgA+ (Wild-type E.coli curli producing strain); Negative control/CsgA- (csgA-knockout E.coli strain); BBa_CsgA (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgAHis1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgAHis2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). <b>A)</b> Biofilm sections obtained by Z-stack acquisitions. <b>B)</b> Biofilm 3D reconstruction using IMARIS® from acquisitions in A). <b>C) </b>Bio-volume quantification and maximum of thickness measurement using COMSTAT2 (ImageJ). The strain marked with a star is significantly different from all others (Tukey’s test, p<0.05).</p> | + | <p>All the strains used are constitutively fluorescent to allow detection with confocal laser microscopy (ZEISS LSM510 META, 40X/1.3OILDIC, laser Argon 4 lines 30 W 458 nm, 477 nm, 488 nm, 514 nm, <a href="https://static.igem.org/mediawiki/2014/7/7e/Culture_confocal_analyse.pdf">See Protocole</a>). Positive control/CsgA+ (Wild-type <i>E. coli</i> curli producing strain); Negative control/CsgA- (csgA-knockout <i>E. coli</i> strain); BBa_CsgA (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgAHis1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgAHis2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). <b>A)</b> Biofilm sections obtained by Z-stack acquisitions. <b>B)</b> Biofilm 3D reconstruction using IMARIS® from acquisitions in A). <b>C) </b>Bio-volume quantification and maximum of thickness measurement using COMSTAT2 (ImageJ). The strain marked with a star is significantly different from all others (Tukey’s test, p<0.05).</p> |
- | <p></br>As no strains carrying our parts show a significant difference with the positive control, our parts insertion doesn’t modify the biofilm formation property. The His-Tag and His2-Tag engineered CsgA doesn’t disturb the curli formation.</p> | + | <p></br>As no strains carrying our parts show a significant difference with the positive control, our parts insertion doesn’t modify the biofilm formation property. <b>The His-Tag and His2-Tag engineered CsgA doesn’t disturb the curli formation.</b></p> |
</p></div></li> | </p></div></li> |
Revision as of 16:58, 17 October 2014