Team:INSA-Lyon/Results

From 2014.igem.org

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<p>Significant differences are indicated using lowercase letters, and different letters indicate significant differences (Tukey’s test, p < 0.05). Error bars represent standard deviations.</p>
<p>Significant differences are indicated using lowercase letters, and different letters indicate significant differences (Tukey’s test, p < 0.05). Error bars represent standard deviations.</p>
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<p>Taken together, these results show that the CsgA- Strain with part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404008">BBa_K1404008</a>8 chelates twice more than strain CsgA- with part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404007">BBa_K1404007</a>. That means that <b>only two His-tags on C-term can improve the natural nickel chelation capacities of CsgA </b>. CsgA with a single His-tag did not perform better than a wild-type CsgA. Potentially, further increasing the amount of His-tags could improve the nickel accumulation capacities of CsgA. </p>
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<p>Taken together, these results show that the CsgA- Strain with part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404008">BBa_K1404008</a> chelates twice more than strain CsgA- with part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404007">BBa_K1404007</a>. That means that <b>only two His-tags on C-term can improve the natural nickel chelation capacities of CsgA </b>. CsgA with a single His-tag did not perform better than a wild-type CsgA. Potentially, further increasing the amount of His-tags could improve the nickel accumulation capacities of CsgA. </p>
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Revision as of 13:49, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Curli characterization


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization