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Team:NTNU Trondheim/Project/Modelling
From 2014.igem.org
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| - | <h3> | + | <h3>Flux balance analysis of the impact of increasing CO2-uptake and introducing Glucose Oxidase into Synechocystis sp. PCC6803 </h3> |
| - | <p> | + | <p> |
| + | To aid in the identification of possible target genes for increasing CO2 uptake in Synechocystis, <a href="http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1003081">the most recent model of the metabolic network in Synechocystis</a> was acquired and analyzed with | ||
| + | <a href="http://www.nature.com/nbt/journal/v28/n3/abs/nbt.1614.html">flux balance analysis</a>. The approach initially chosen was simply to fix the CO2 uptake flux above that achieved in the optimal FBA solution, and examining the effect on the growth rate (figure 1). | ||
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| + | <div class="col4"><a href="https://static.igem.org/mediawiki/2014/0/0b/Growth.jpg"> <img src="https://static.igem.org/mediawiki/2014/0/0b/Growth.jpg" width="500"> | ||
| + | <p style="text-align:center; color:black; "><b> Figure:</b> Synechocystis growth rate as a function of CO2 uptake flux in an optimal FBA solution.</p> </div> | ||
| + | As can be seen, the growth rate increases from 0 as the CO2 uptake flux is increased from zero to its optimal value (1.19), and decreases after this point. | ||
| + | <math>\times</math> | ||
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| + | </p> | ||
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Revision as of 16:33, 17 October 2014
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Team:NTNU_Trondheim/Home
From 2014.igem.org
Modelling
Flux balance analysis of the impact of increasing CO2-uptake and introducing Glucose Oxidase into Synechocystis sp. PCC6803
To aid in the identification of possible target genes for increasing CO2 uptake in Synechocystis, the most recent model of the metabolic network in Synechocystis was acquired and analyzed with flux balance analysis. The approach initially chosen was simply to fix the CO2 uptake flux above that achieved in the optimal FBA solution, and examining the effect on the growth rate (figure 1).
As can be seen, the growth rate increases from 0 as the CO2 uptake flux is increased from zero to its optimal value (1.19), and decreases after this point.
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