Team:York/Cake

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<li><a href="https://2014.igem.org/Team:York">Home</a></li>
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<li> <a href="https://2014.igem.org/Team:York/Sustainability">Sustainability</a></li>
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<li><a href="https://2014.igem.org/Team:York/Notebook">Notebook</a></li>
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<h1>Constructs</h1>
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<h1>Surveys</h1>
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<img class="img-responsive img-max-650" src="https://static.igem.org/mediawiki/2014/a/ae/York_Constructs.jpg">
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<p>Through our surveys, we aimed to determine whether age, gender, nationality, scientific literacy and religious and political views significantly influence public opinion on the topic of GMOs, GMO based projects and portrayal of GMO in the media.</p>
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<br><br>
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<h3>1. Environmental Protection</h3><br>
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<div class="col-md-3">
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<p>91% of respondents considered environmental protection important or extremely important. For this question, significant differences between groups were only observed between genders, with 78% of females saying that environmental protection is extremely important, compared to only 40% of males (figure 1.).</p>
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<ul class="nav nav-tabs" role="tablist" id="myTab">
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    <li class="active"><a href="#one" role="tab" data-toggle="tab">pYodA</a></li>
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    <li><a href="#two" role="tab" data-toggle="tab">NRAMP</a></li>
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    <li><a href="#three" role="tab" data-toggle="tab">CysP</a></li>
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    <li><a href="#four" role="tab" data-toggle="tab">SpPCS</a></li>
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    <li><a href="#five" role="tab" data-toggle="tab">Gsh1*</a></li>
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    <li><a href="#six" role="tab" data-toggle="tab">CysE*</a></li>
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<small><strong>Figure 1. Responses the the question ‘In your opinion, protecting the environment is:’</strong></small><br><br>
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<h2>pYodA</h2>
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<h3>2. Opinion on Environmental Remediation Projects and Initiatives</h3 ><br>
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<p>
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<p>We asked our respondents whether they would support or discourage environmental remediation projects and initiatives. Significant differences were observed between age groups, genders and Western and Eastern Europeans.</p>
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<b>Name:</b> pYodA (ZinTp) <br>
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<p> 95% of respondents under the age of 24 said that they would support or strongly support environmental remediation projects, compared to only 73% of those over 24 (figure 2.).</p>
-
<b>Organism:</b> <i>Escherichia Coli</i> <br>
+
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<b>Normal Function:</b> pYodA is a cadmium-induced promoter that activates the yodA(zinT) gene, leading to the production of ZinT metal-binding protein.<br>
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<b>Aim:</b> We are using pYodA in an alternative way; to regulate the expression of the CysP gene (sulfate transporter) and the NRAMP gene (Cadmium transporter). Due to using pYodA, the expression of these two genes will be regulated by the concentration of Cadmium in the extracellular environment. The over-production of Cysteine will only occur when the concentration of Cadmium reaches the sensitivity threshold of pYodA. Due to cysteine production being both energetically demanding and toxic at high concentrations, we do not want these genes to be expressed constitutively. <br>
+
-
<b>Characterisation:</b> In order to characterise pYodA, we will couple it with a GFP gene. This will allow us to calculate the sensitivity threshold of pYodA and measure the amount of cadmium that can be chelated at various concentrations. <br>
+
-
<b>Literature:</b>
+
-
<ol>
+
-
<li>http://mic.sgmjournals.org/content/148/12/3801.long</li>
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<li>http://www.uniprot.org/uniprot/F4VWH2</li>
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<li>http://sbkb.org/uid/F4VWH2/uniprot#structures</li>
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<li>http://www.jbc.org/content/278/44/43728</li>
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<li>http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=G7061</li>
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<img src="https://static.igem.org/mediawiki/2014/0/0b/Figure_2igem_york.png" class="headshot img-responsive">
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<h2>NRAMP</h2>
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<small><strong>Figure 2. Responses to the question “Would you support or discourage environmental remediation prodjects and initiatives?” Based on age.</strong></small><br><br>
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<p>
+
-
<b>Gene:</b> mntH<br>
+
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<b>Organism:</b> <i>Escherichia Coli</i><br>
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<b>Protein:</b> NRAMP<br>
+
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<b>Function:</b> NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.<br>
+
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<b>Aim:</b> We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.<br>
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<b>Expression:</b> We can run assays measuring the concentration of cadmium in the environment. If NRAMP is active, the concentration of cadmium should decrease.<br></p></div>
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<p>At 92%, females are also more likely than males to support or strongly support environmental protection initiatives, who would only do so 80% of the time (figure 3.). </p>
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<h2>CysP</h2>
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<img src="https://static.igem.org/mediawiki/2014/7/7e/Figure_3york_igem.png" class="headshot img-responsive">
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<p>
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<small><strong>Figure 3. Responses to the question “Would you support or discourage environmental remediation projects and initiatives?” Based on gender.</small></strong><br><br>
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<b>Gene:</b> cysP (AKA YlnA) <br>
+
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<b>Organism:</b> <i>Bacillus Subtilis</i> <br>
+
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<b>Protein:</b> CysP <br>
+
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<b>Function:</b> This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space. <br>
+
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<b>Aim:</b> We want to overexpress this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium. <br>
+
-
<b>Expression:</b> We could run assays measuring the concentration of sulfate in the environment containing our bacteria. <br>
+
-
</p>
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<p>As opposed to 98% of Western European respondents, only 75% of those from Eastern Europe would support projects aiming to protect the environment (figure 4.) </p>
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<img src="https://static.igem.org/mediawiki/2014/b/b4/Figure_4igem_york.png" class="headshot img-responsive">
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<h2>CysE*</h2>
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<small><strong>Figure 4.  Responses to the question “Would you support or discourage environmental remediation projects and initiatives?” Based on region of origin.</small></strong><br><br>
-
<p>
+
 
-
<b>Original Gene:</b> cysE<br>
+
<h3>3. Opinion on Environmental Remediation Projects that Rely on GMOs</h3>
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<b>Mutant Gene:</b> cysE*<br>
+
<p>Although 85% of our respondents said that they would support, or strongly support environmental remediation projects and initiatives, the number dropped to 60% when respondents were told that these projects would use genetically modified organisms.  Compared to the previous question, where there were significant differences between groups based on age, gender and nationality, in this case the only significant differences are based on religious affiliation(figure 5) and scientific literacy (figure 6)</p>
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<b>Organism:</b> <i>Escherichia Coli</i> <br>
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<b>Protein:</b> CysE(SAT)<br>
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<img src="https://static.igem.org/mediawiki/2014/e/e3/Figure_5igem_york.png" class="headshot img-responsive">
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<b>Normal function:</b> To catalyse the acetylation of L-serine (the first step in cysteine biosynthesis)<br>
+
<small><strong>Figure 5.  Responses to the question “Would you support or discourage environmental remediation projects and initiatives that rely on the use of GMOs?” (Opinion based on religious affiliation)</strong></small><br><br>  
-
<b>Function:</b> Serine acetyltransferase (CysE) carries out the first step in cysteine biosynthesis; it  catalyses the acetylation of L-serine which generates O-acetyl-L-serine. Cysteine itself strongly inhibits the activity of serine acetyltransferase by binding to the serine-binding site. This inhibition depends on the protein's carboxy terminus, and has been localized to Met-256 specifically. Due to cysteine production being both energetically demanding and toxic at high concentrations, the cell does not want to produce cysteine constitutively.<br>
+
 
-
<b>Aim:</b> To over-produce cysteine.To fulfill this aim, we need to remove negative feedback from cysteine biosynthesis. To do this, we are using a mutant CysE gene (CysE*). Our mutant CysE gene will produce a protein that has a single amino acid substitution: Met-256 will be replaced by Ile by changing the corresponding AUG codon to AUC. This single amino-acid substitution will alter the three dimensional shape of the serine-binding site in our acetyltransferase. Changing the shape of the serine-binding site prevents cysteine from binding to it. Thus, our acetyltransferase will not be inhibited by cysteine. As a result, we will be able to over-produce cysteine in our cell.<br>
+
 
-
<b>Method:</b> We had the cysE* gene synthesised to produce the mutant CysE* protein.<br>
+
<p>For the purposes of this report, we have divided our respondents into two categories: religious and non-religious. However, we plan on carrying out more in depth analysis of our data in the near future, when we will further divide the “religious” category based on the specific affiliation indicated by our responders.</p>
-
<b>Literature:</b>  
+
<p>As far as scientific literacy is concerned, we asked our respondents to select the level of scientific literacy they possess from the following:</p>
<ol>
<ol>
-
<li>http://aem.asm.org/content/66/10/4497.full</li>
+
<li>Little or no contact with science and no understanding of the scientific method.
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<li>Denk D., Bock A. <i>J. Gen. Microbiol</i>, 1987</li>
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<li>Occasional contact with science(eg. media) and a basic understanding of the scientific method.
-
</ol></p></div>
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<li>Contact with science through secondary education(eg. high school science classes) or equivalent level and a moderate understanding of the scientific method.
 +
<li>Contact with science through higher education(eg. university science degree) or equivalent level, ability to read and understand articles in scientific journals and a good understanding of the scientific method.
 +
<li>An understanding of science equivalent to having a career in a scientific field, ability to read and understand articles in scientific journals, a very good understanding and experience of applying the scientific method.</ol>
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<h2>Gsh1*</h2>
 
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<p>
 
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<b>Original Gene:</b> gsh1/gshA<br>
 
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<b>Organism:</b> <i>Saccharomyces cerevisiae</i><br>
 
-
<b>Protein:</b> Glutamate-Cysteine Ligase (previously known as gamma-glutamylcysteine synthetase)<br>
 
-
<b>Function:</b> Gamma glutamylcysteine synthetase catalyzes the first step in glutathione (GSH) biosynthesis;<br>
 
-
L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi<br>
 
-
Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress.<br>
 
-
<b>Aim:</b> We can use the overproduced cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, we need glutamate-cysteine ligase to catalyse the reaction. We can overexpress GSH1* using the pYodA promoter. <br>
 
-
<b>Literature:</b><ol><li>http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C</li></ol></p></div>
 
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<div class="tab-pane" id="four" class="collapse">
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<p>Although there is no significant difference between levels 2 and 3, respondents who have identified with level 4  scientific literacy were significantly more likely to support environmental projects that relied on GMOs and over 80% of those who identified with level 5 scientific literacy said they would support such initiatives. None of our respondents have selected level 1 scientific literacy.</p>
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<h2>spPCS</h2>
+
 
-
<p>
+
<img src="https://static.igem.org/mediawiki/2014/b/bf/Figure_6igem_york.png" class="headshot img-responsive">
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<b>Gene:</b> spPCS<br>
+
<small><strong>Figure 6. Responses to  the question “Would you support or discourage environmental remediation projects and initiatives that rely on the use of GMOs?” (Opinion based on scientific literacy level)</small></strong><br><br>
-
<b>Organism:</b> <i>Schizosaccharomyces pombe</i><br>
+
 
-
<b>Function:</b> The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group  to synthesise phytochelatins.<br>
+
 
-
<b>Aim:</b> Overexpression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in  taking up cadmium.<br>
+
<p>Our respondents were asked to what extent they had researched GMOs before taking the survey. Our findings show that, as expected, the responders who had done extensive research into the topic were more likely to “Strongly support” or “Strongly discourage” environmental projects that relied on GMOs.  Responders who had done little to no research into GMOs were less likely to take a stance on this issue, with 40-50% of them saying that they would neither support nor discourage these projects (figure 7).</p>
-
<b>Literature</b><ol><li>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/</li></ol></p></div>
+
 
 +
<img src="https://static.igem.org/mediawiki/2014/8/82/Figure_7york_igem.png" class="headshot img-responsive">
 +
<small><strong>Figure 7. Responses to the question “Would you support or discourage environmental remediation projects and initiatives that rely on the use of GMOs?” (Opinion based on previous research)</small></strong><br><br>
 +
 
 +
 
 +
<h3>4. Opinion on the Impact of Various Factors on One's Stance towards GMOs</h3><br>
 +
<p>Respondents were asked to rate on a scale from 1(lowest) to 5(highest) the impact that they believe certain factors have had on their attitude towards GMOs. Our findings show that up to 70% of our respondents believe that their opinion was shaped by scientific consensus as well as independent research. At the other end of the spectrum are religious and political affiliation, with less than 10% of respondents saying that these factors have had an impact on their attitude towards GMOs (figure 8).</p>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2014/5/5b/Figure_8york_igem.png" class="headshot img-responsive">
 +
<small><strong>Figure 8. Responses to the question “On a scale from 1(lowest) to 5(highest), how would you rate the impact that each of the below has had on your attitude towards GMOs?”</small></strong><br><br>
 +
 
 +
 
 +
<h3>5. Additional Comments and Opinions</h3><br></li>
 +
<p>Our responders were given the opportunity to comment on our survey and on the topic of GMOs. A significant number have expressed their disappointment in the way GMOs are portrayed in the media and in how the concept of GMOs is understood by the public:</p><ul>
 +
<li>“We urgently need more scientifically accurate info throughout the media! Sensationalist fear mongering is a poison and the antidote is lacking.
 +
<li>“GMO subject is poorly understood by general public and superficially treated by media “
 +
<li>“Having talked to non scientific friends I have found that the vast number are unaware that plants and other organisms have genes. As a consequence they assume that we are putting human genes into foreign bodies and "making them humanoids" “
 +
<li>“Generally considered to much more of an unknown terror in England than in America (where I presume you are based?). Viewed by the general public, by spin in the media, as unnatural and hazardous but I think it surely it has to be the future for crop development in a world where population growth is far outstripping food production. “
 +
<li>“Depending on what continent your survey participants are from, some of the answers may have entirely opposite meanings: in the U.S. GMOs are largely accepted as useful and safe (and are therefore legal) while in the E.U. there is a lot of misinformed public opposition to them (and also some legislation aiming to limit or prohibit their use)."</ul>
 +
<p>Some of the comments have also expressed the view that the problem with GMOs is not the organism itself, but the way it is used by corporations:</p><ul>
 +
<li>“I don't mind GMOs designed to thrive in harsh environment or have a stable fertile crop. But I do have a problem with copywriting them, designing them to be drowned in pesticides or making the crops sterile as to require the re-purchasing of seed stock ever year. So really it's the business/politics not the science that bothers me. “
 +
<li>“I think a major factor is the gap between the problems of uses of the technology and whether it has inherent issues - the exploitation of GMOs by major companies is a real problem but has led to excessive fear of the technology. “</ul>
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Revision as of 13:12, 17 October 2014

Team York 2014


Surveys


Through our surveys, we aimed to determine whether age, gender, nationality, scientific literacy and religious and political views significantly influence public opinion on the topic of GMOs, GMO based projects and portrayal of GMO in the media.

1. Environmental Protection


91% of respondents considered environmental protection important or extremely important. For this question, significant differences between groups were only observed between genders, with 78% of females saying that environmental protection is extremely important, compared to only 40% of males (figure 1.).

Figure 1. Responses the the question ‘In your opinion, protecting the environment is:’

2. Opinion on Environmental Remediation Projects and Initiatives


We asked our respondents whether they would support or discourage environmental remediation projects and initiatives. Significant differences were observed between age groups, genders and Western and Eastern Europeans.

95% of respondents under the age of 24 said that they would support or strongly support environmental remediation projects, compared to only 73% of those over 24 (figure 2.).

Figure 2. Responses to the question “Would you support or discourage environmental remediation prodjects and initiatives?” Based on age.

At 92%, females are also more likely than males to support or strongly support environmental protection initiatives, who would only do so 80% of the time (figure 3.).

Figure 3. Responses to the question “Would you support or discourage environmental remediation projects and initiatives?” Based on gender.

As opposed to 98% of Western European respondents, only 75% of those from Eastern Europe would support projects aiming to protect the environment (figure 4.)

Figure 4. Responses to the question “Would you support or discourage environmental remediation projects and initiatives?” Based on region of origin.

3. Opinion on Environmental Remediation Projects that Rely on GMOs

Although 85% of our respondents said that they would support, or strongly support environmental remediation projects and initiatives, the number dropped to 60% when respondents were told that these projects would use genetically modified organisms. Compared to the previous question, where there were significant differences between groups based on age, gender and nationality, in this case the only significant differences are based on religious affiliation(figure 5) and scientific literacy (figure 6)

Figure 5. Responses to the question “Would you support or discourage environmental remediation projects and initiatives that rely on the use of GMOs?” (Opinion based on religious affiliation)

For the purposes of this report, we have divided our respondents into two categories: religious and non-religious. However, we plan on carrying out more in depth analysis of our data in the near future, when we will further divide the “religious” category based on the specific affiliation indicated by our responders.

As far as scientific literacy is concerned, we asked our respondents to select the level of scientific literacy they possess from the following:

  1. Little or no contact with science and no understanding of the scientific method.
  2. Occasional contact with science(eg. media) and a basic understanding of the scientific method.
  3. Contact with science through secondary education(eg. high school science classes) or equivalent level and a moderate understanding of the scientific method.
  4. Contact with science through higher education(eg. university science degree) or equivalent level, ability to read and understand articles in scientific journals and a good understanding of the scientific method.
  5. An understanding of science equivalent to having a career in a scientific field, ability to read and understand articles in scientific journals, a very good understanding and experience of applying the scientific method.

Although there is no significant difference between levels 2 and 3, respondents who have identified with level 4 scientific literacy were significantly more likely to support environmental projects that relied on GMOs and over 80% of those who identified with level 5 scientific literacy said they would support such initiatives. None of our respondents have selected level 1 scientific literacy.

Figure 6. Responses to the question “Would you support or discourage environmental remediation projects and initiatives that rely on the use of GMOs?” (Opinion based on scientific literacy level)

Our respondents were asked to what extent they had researched GMOs before taking the survey. Our findings show that, as expected, the responders who had done extensive research into the topic were more likely to “Strongly support” or “Strongly discourage” environmental projects that relied on GMOs. Responders who had done little to no research into GMOs were less likely to take a stance on this issue, with 40-50% of them saying that they would neither support nor discourage these projects (figure 7).

Figure 7. Responses to the question “Would you support or discourage environmental remediation projects and initiatives that rely on the use of GMOs?” (Opinion based on previous research)

4. Opinion on the Impact of Various Factors on One's Stance towards GMOs


Respondents were asked to rate on a scale from 1(lowest) to 5(highest) the impact that they believe certain factors have had on their attitude towards GMOs. Our findings show that up to 70% of our respondents believe that their opinion was shaped by scientific consensus as well as independent research. At the other end of the spectrum are religious and political affiliation, with less than 10% of respondents saying that these factors have had an impact on their attitude towards GMOs (figure 8).

Figure 8. Responses to the question “On a scale from 1(lowest) to 5(highest), how would you rate the impact that each of the below has had on your attitude towards GMOs?”

5. Additional Comments and Opinions


Our responders were given the opportunity to comment on our survey and on the topic of GMOs. A significant number have expressed their disappointment in the way GMOs are portrayed in the media and in how the concept of GMOs is understood by the public:

  • “We urgently need more scientifically accurate info throughout the media! Sensationalist fear mongering is a poison and the antidote is lacking. “
  • “GMO subject is poorly understood by general public and superficially treated by media “
  • “Having talked to non scientific friends I have found that the vast number are unaware that plants and other organisms have genes. As a consequence they assume that we are putting human genes into foreign bodies and "making them humanoids" “
  • “Generally considered to much more of an unknown terror in England than in America (where I presume you are based?). Viewed by the general public, by spin in the media, as unnatural and hazardous but I think it surely it has to be the future for crop development in a world where population growth is far outstripping food production. “
  • “Depending on what continent your survey participants are from, some of the answers may have entirely opposite meanings: in the U.S. GMOs are largely accepted as useful and safe (and are therefore legal) while in the E.U. there is a lot of misinformed public opposition to them (and also some legislation aiming to limit or prohibit their use)."

Some of the comments have also expressed the view that the problem with GMOs is not the organism itself, but the way it is used by corporations:

  • “I don't mind GMOs designed to thrive in harsh environment or have a stable fertile crop. But I do have a problem with copywriting them, designing them to be drowned in pesticides or making the crops sterile as to require the re-purchasing of seed stock ever year. So really it's the business/politics not the science that bothers me. “
  • “I think a major factor is the gap between the problems of uses of the technology and whether it has inherent issues - the exploitation of GMOs by major companies is a real problem but has led to excessive fear of the technology. “