Team:Evry/Notebook/Transformation/15-07-2014

From 2014.igem.org

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<li>2000V
<li>2000V
and sowed cells on MB 1X+Cam 1/1000 after 2hours of incubation.<br>
and sowed cells on MB 1X+Cam 1/1000 after 2hours of incubation.<br>
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Latest revision as of 12:54, 17 October 2014

Picture

Transformation of Pseudovibrio with PSB1C3 (2)
We made electrocompetent Pseudovibrio and transform them by electroporation with

  • 1µL of palsmid (33ng/µL)
  • 50µL of cells
  • 2000V and sowed cells on MB 1X+Cam 1/1000 after 2hours of incubation.

    July 15

    • Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Transformation/15-07-2014"