Team:Melbourne/Protocol
From 2014.igem.org
(Difference between revisions)
Line 542: | Line 542: | ||
<p> </p> | <p> </p> | ||
<p><h3>PROTEIN PURIFICATION (D)</h3></p> | <p><h3>PROTEIN PURIFICATION (D)</h3></p> | ||
- | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/e1/ | + | <p><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/e1/MELBOURNE_D1V1_Batch_Purification_of_His-Tagged_Proteins.pdf">D1V1 Column Purification of His-Tagged Proteins</a></p> |
<p> </p> | <p> </p> | ||
<p><h3>PROTEIN ANALYSIS (E)</h3></p> | <p><h3>PROTEIN ANALYSIS (E)</h3></p> |
Revision as of 12:58, 17 October 2014
Home | Team | Official Team Profile | Project | Notebook | Protocol | Safety | Attributions | Public Outreach |
PROTOCOLS
PREPARING CELLS FOR TRANSFORMATION (A)
A1V1 Preparing Competent Cells
A2V1 Preparation of Liquid Broth Media
PREPARING PLASMIDS FOR TRANSFORMATION (B)
B6V1 RE-Based Colony Screening
TRANSFORMATION AND PROTEIN EXPRESSION (C)
PROTEIN PURIFICATION (D)
D1V1 Column Purification of His-Tagged Proteins
PROTEIN ANALYSIS (E)
OTHER (F)