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Team:Evry/Notebook/Transformation/15-07-2014
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<u> <b> <big><FONT COLOR=#003333>Transformation of <i>Pseudovibrio</i> with PSB1C3</font></big></b> </u> <br> | <u> <b> <big><FONT COLOR=#003333>Transformation of <i>Pseudovibrio</i> with PSB1C3</font></big></b> </u> <br> | ||
| - | + | We made electrocompetent <i>Pseudovibrio</i> and transform them by electroporation with | |
| - | + | <li>1µL of palsmid (33ng/µL) | |
| + | <li>50µL of cells | ||
| + | <li>2000V | ||
| + | and sowed cells on MB 1X+Cam 1/1000 after 2hours of incubation.<br> | ||
</p> | </p> | ||
| - | <span class="cd-date">July | + | <span class="cd-date">July 15</span> |
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 12:50, 17 October 2014
Transformation of Pseudovibrio with PSB1C3
We made electrocompetent Pseudovibrio and transform them by electroporation with
July 15
