Team:Groningen/Template/MODULE/notebook

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Revision as of 15:29, 17 October 2014

Notebook > Appendix
 
 
 
Primers for making BioBricks out of the nisin operon
 
Primer Sequence
NisA forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA
NisA reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA
NisB forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA
NisB reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT
NisT forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
NisT reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
NisC forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA
NisC reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC
NisI forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT
NisI reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG
NisP forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT
NisP reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
NisRK forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT
NisRK reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA
NisFEG forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
NisFEG reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
PNisI+RBS forward GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT
PNisI+RBS reverse TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA
 
 
 
 
Primers for making the new sfGFP(Bs) BioBrick
 
Primer Sequence
sfGFP(Bs) forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT
sfGFP(Bs) reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT
 
 
 
 
Primers for making BioBricks out of genes with illegal restriction sites
 
The primers are denoted with the position of the illegal restriction site that they should remove. Illegal EcoRI sites are abbreviated with an E, XbaI sites with an X, SpeI site with an S and PstI sites with a P.
 
Gene Primer Sequence
NisT Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
  S980 reverse TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA
  S980 forward TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA
  S1210 reverse CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC
  S1210 forward GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
  Suffix forward ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisP Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT
  P225 reverse AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT
  P225 forward AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT
  E347 reverse AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT
  E347 forward AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT
  E1657 reverse CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA
  E1657 forward TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
  Suffix forward AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisFEG Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
  P186 reverse TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC
  P186 forward GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA
  E336/X349 reverse CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA
  E336/X349 forward TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
  Suffix forward TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
 
 
 
 

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