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Team:DTU-Denmark/Primers
From 2014.igem.org
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Revision as of 12:37, 17 October 2014
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At the Giant Jamboree, we received a gold medal and an Interlab Study Award. We also won the Overgrad Best Parts Collection.
Below is a list of primers used in the experimental work
| Primer name | 5'-sequence-3' | Description |
| VF2 | TGCCACCTGACGTCTAAGAA | Primer for sequencing insert in standard backbone |
| VR | ATTACCGCCTTTGAGTGAGC | Reverse primer for sequencing insert in standard backbone |
| P3 | ATTCCGCUAAGGATGATTTCTGGAATTCGCGG | Primer attaching on backbone upstream BioBrick prefix |
| P4 | ACCTTGCCCUTTTTTGCCGGACTGCAGCGGC | Primer attaching on backbone downstream BioBrick suffix |
| P5 | TGGCGCCCGAACAGGGACTTGA | Reverse primer attaching upstream terminator for in vitro template amplification |
| P6 | TAATACGACTCACTATAGGGAGAGCCCGGATAGCTCAGTCGGT | Primer with T7 promoter tail for in vitro transcription template aplification |
| P7 | TTGCCGGACTGCAGCGGCCGCTACTAGTATGGCGCCCGAACAGGGACTT | Reverse primer attaching upstream of the terminator with a primer tail including BioBrick suffix |
