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Team:DTU-Denmark/Primers
From 2014.igem.org
(Difference between revisions)
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<tr><td>VF2</td><td>TGCCACCTGACGTCTAAGAA</td><td>Primer for sequencing insert in standard backbone</td></tr> | <tr><td>VF2</td><td>TGCCACCTGACGTCTAAGAA</td><td>Primer for sequencing insert in standard backbone</td></tr> | ||
| + | <tr><td>VR</td><td>ATTACCGCCTTTGAGTGAGC</td><td>Reverse primer for sequencing insert in standard backbone</td></tr> | ||
| + | <tr><td>P3</td><td>ATTCCGCUAAGGATGATTTCTGGAATTCGCGG</td><td>Primer attaching on backbone upstream BioBrick prefix</td></tr> | ||
| + | <tr><td>P4</td><td>ACCTTGCCCUTTTTTGCCGGACTGCAGCGGC</td><td>Primer attaching on backbone downstream BioBrick suffix</td></tr> | ||
| + | |||
| + | <tr><td>P5</td><td>TGGCGCCCGAACAGGGACTTGA</td><td> Reverse primer attaching upstream terminator for <i>in vitro</i> template amplification</td></tr> | ||
| + | |||
| + | <tr><td>P6</td><td>TAATACGACTCACTATAGGGAGAGCCCGGATAGCTCAGTCGGT</td><td>Primer with T7 promoter tail for <i>in vitro</i> transcription template aplification</td></tr> | ||
| + | |||
| + | <tr><td>P7</td><td>TTGCCGGACTGCAGCGGCCGCTACTAGTATGGCGCCCGAACAGGGACTT</td><td>Reverse primer attaching upstream of the terminator with a primer tail including BioBrick suffix</td></tr> | ||
</table> | </table> | ||
Revision as of 12:35, 17 October 2014
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At the Giant Jamboree, we received a gold medal and an Interlab Study Award. We also won the Overgrad Best Parts Collection.
Below is a list of primers used in the experimental work
| Primer name | 5'-sequence-3' | Description |
| VF2 | TGCCACCTGACGTCTAAGAA | Primer for sequencing insert in standard backbone |
| VR | ATTACCGCCTTTGAGTGAGC | Reverse primer for sequencing insert in standard backbone |
| P3 | ATTCCGCUAAGGATGATTTCTGGAATTCGCGG | Primer attaching on backbone upstream BioBrick prefix |
| P4 | ACCTTGCCCUTTTTTGCCGGACTGCAGCGGC | Primer attaching on backbone downstream BioBrick suffix |
| P5 | TGGCGCCCGAACAGGGACTTGA | Reverse primer attaching upstream terminator for in vitro template amplification |
| P6 | TAATACGACTCACTATAGGGAGAGCCCGGATAGCTCAGTCGGT | Primer with T7 promoter tail for in vitro transcription template aplification |
| P7 | TTGCCGGACTGCAGCGGCCGCTACTAGTATGGCGCCCGAACAGGGACTT | Reverse primer attaching upstream of the terminator with a primer tail including BioBrick suffix |
