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Team:Evry/Notebook/Transformation/18-07-2014
From 2014.igem.org
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<u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice of the promoter</font></big></b> </u> <br> | <u> <b> <big><FONT COLOR=#003333>Construction of a new plasmid - Choice of the promoter</font></big></b> </u> <br> | ||
We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of <i>Pseudovibrio FOBEG1</i>.<br> | We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of <i>Pseudovibrio FOBEG1</i>.<br> | ||
| - | PCR with primers 5 and 6 (see primers table in <a href="https://2014.igem.org/Team:Evry/Notebook/Protocols">Protocols</a>). | + | PCR with primers 5 and 6 (see primers table in <a href="https://2014.igem.org/Team:Evry/Notebook/Protocols">Protocols</a>), Q5 high fidelity and TM=55°C. |
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Revision as of 12:33, 17 October 2014
Construction of a new plasmid - Choice of the promoter
We chose to amplify the tkt promoter of the transkelotase gene. We find this strong constitutive promoter with the genome browser on the genome of Pseudovibrio FOBEG1.
PCR with primers 5 and 6 (see primers table in Protocols), Q5 high fidelity and TM=55°C.
