Team:Groningen/Template/MODULE/projects/parts/biobricktable

From 2014.igem.org

(Difference between revisions)

Revision as of 10:59, 17 October 2014

iGEM Groningen 2014 assembled parts

EMPTY	Part	Type	Description	Description	Designer	Length
EMPTY	BBa_K1365000	Coding	nisA	Nisin precursor. Should be used in combination with the nisin gene cluster (e.g. Lactococcus lactis NZ9800).	Sandra Mous	174
EMPTY	BBa_K1365003	Coding	nisC	Protein needed in the cyclization of the nisin precursor. Part of the nisin gene cluster.	Sandra Mous	1245
EMPTY	BBa_K1365006	composite	nisRK	Two proteins involved in the recognition of nisin and positive feedback regulation to induce the pNisA promoter. This biobrick is part of the nisin gene cluster.	Sandra Mous	2023
EMPTY	BBa_K1365020	Coding	sfGFP	Superfolder GFP is a more table protein than more communly used GFP. It gives an higher fluorescence as well1.	Rick Elbert	1625
EMPTY	BBa_K1365100	Device	EMPTY	Dual action system against S. aureus. System made for L. lactis and codes for nisin precursor and the biofilm degradeding enzyme DspB. The latter is fused with an USP45sp for secretion from the cell. This device has an P2 operon and this thus transcriped after binding from p-AgrA.	Rick Elbert	1625
EMPTY	BBa_K1365111	Coding	ssUsp45-dspB	DispersinB fused to an Usp45sp. The dspB enzyme is secreted from the cell via the usp system of L. lactis2.	Rick Elbert	1170
EMPTY	BBa_K1365120	Device	EMPTY	Dual action system against P. aeruginosa. System is made for L. lactis and includes the genes for autoinducer inactivation enzyme A (AiiA) and Dispersin B (DspB).	Rick Elbert	2350
EMPTY	BBa_K1365130	Device	EMPTY	EMPTY	Rick Elbert	EMPTY
EMPTY	BBa_K1365169	Coding	ssUsp45-aiiA	Autoinducer inactivation enzyme A (AiiA) gene fused to signal sequence for the usp45 secretion pathway.	Rick Elbert	834
EMPTY	BBa_K1365301	Plasmid	pIL253³	Plasmid for L. lactis including the biobrick compatible VF2 and RV primer binding sites, prefix and suffix. No illegal restriction sites.	Lisa Hielkema	6230
EMPTY	BBa_K1365555	Compesition	RBS-sfGFP-dt	Compesition of the superfolder GFP combined with RBS and double terminator (dt). This part was used to assemble with different promoters used for characterization experiments.	Aakanksha Saraf	874
EMPTY	BBa_K1365556	Compesition	RBS-nisA-dt	Compesition of the precursor nisA combined with an RBS and double terminator (dt). This part was assembled with a range of promotor to be used for characterization experiments.	Aakanksha Saraf	328
EMPTY	BBa_K1365557	Reporter	EMPTY	ESuperfolder GFP under a constitituve promoter. Desingend for the use in L. lactis and E. coli.PTY	Aakanksha Saraf	915
EMPTY	BBa_K1365997	Device	EMPTY	AHL sensor. The LasR membrane protein is produced by this device.	Chandhuru Jagadeesan	1003
EMPTY	BBa_K1365998	Signaling	EMPTY	AHL generator. This part can be used to mimic the presence of AHL producing bacteria P. aeruginosa.	Chandhuru Jagadeesan	892
EMPTY	BBa_K1365999	compesition	RBS-GFP-dt	Composition of ribosome binding site, green fluorescence protein and double terminator. Brick is both L. lactis as E. coli compatible.	Chandhuru Jagadeesan	873
EMPTY	EMPTY	EMPTY	EMPTY	EMPTY	EMPTY	EMPTY

@@ Line 32: / Line 32: @@
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365020</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365020</td><td>Coding</td><td>sfGFP</td><td>Superfolder GFP is a more table protein than more communly used GFP. It gives an higher fluorescence as well1.</td><td>Rick Elbert</td><td>1625</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365100</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365100</td><td>Device</td><td>EMPTY</td><td>Dual action system against S. aureus. System made for L. lactis and codes for nisin precursor and the biofilm degradeding enzyme DspB. The latter is fused with an USP45sp for secretion from the cell. This device has an P2 operon and this thus transcriped after binding from p-AgrA.</td><td>Rick Elbert</td><td>1625</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365111</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365111</td><td>Coding</td><td>ssUsp45-dspB</td><td>DispersinB fused to an Usp45sp. The dspB enzyme is secreted from the cell via the usp system of L. lactis2.</td><td>Rick Elbert</td><td>1170</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365120</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365120</td><td>Device</td><td>EMPTY</td><td>Dual action system against P. aeruginosa. System is made for L.  lactis and includes the genes for autoinducer inactivation enzyme A (AiiA) and Dispersin B (DspB).</td><td>Rick Elbert</td><td>2350</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365130</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365130</td><td>Device</td><td>EMPTY</td><td>EMPTY</td><td>Rick Elbert</td><td>EMPTY</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365169</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365169</td><td>Coding</td><td>ssUsp45-aiiA</td><td>Autoinducer inactivation enzyme A (AiiA) gene fused to signal sequence for the usp45 secretion pathway.</td><td>Rick Elbert</td><td>834</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365301</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365301</td><td>Plasmid</td><td>pIL253<sup>3</sup></td><td>Plasmid for L. lactis including the biobrick compatible VF2 and RV primer binding sites, prefix and suffix. No illegal restriction sites.</td><td>Lisa Hielkema</td><td>6230</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365555</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365555</td><td>Compesition</td><td>RBS-sfGFP-dt</td><td>Compesition of the superfolder GFP combined with RBS and double terminator (dt). This part was used to assemble with different promoters used for characterization experiments.</td><td>Aakanksha Saraf</td><td>874</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365556</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365556</td><td>Compesition</td><td>RBS-nisA-dt</td><td>Compesition of the precursor nisA combined with an RBS and double terminator (dt). This part was assembled with a range of promotor to be used for characterization experiments.</td><td>Aakanksha Saraf</td><td>328</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365557</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365557</td><td>Reporter</td><td>EMPTY</td><td>ESuperfolder GFP under a constitituve promoter. Desingend for the use in L. lactis and E. coli.PTY</td><td>Aakanksha Saraf</td><td>915</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365997</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365997</td><td>Device</td><td>EMPTY</td><td>AHL sensor. The LasR membrane protein is produced by this device. </td><td>Chandhuru Jagadeesan</td><td>1003</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365998</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365998</td><td>Signaling</td><td>EMPTY</td><td>AHL generator. This part can be used to mimic the presence of AHL producing bacteria P. aeruginosa.</td><td>Chandhuru Jagadeesan</td><td>892</td>
 </tr>
 <tr>
-<td>EMPTY</td><td>BBa_K1365999</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>
+<td>EMPTY</td><td>BBa_K1365999</td><td>compesition</td><td>RBS-GFP-dt</td><td>Composition of ribosome binding site, green fluorescence protein and double terminator. Brick is both L. lactis as E. coli compatible.</td><td>Chandhuru Jagadeesan</td><td>873</td>
 </tr>
 <tr>