Team:Groningen/Template/MODULE/Notebook/bandage/week3

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21 July - 25 July
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July 21- July 25
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Growing new overnight cultures and creating some chemicals for the first attempt of pouring some cells in a Gel.
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Growing a new overnight cultures and preparing the chemicals for the first polyacrylamide gels with <i>L. lactis</i> cells
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Creating our first polyacrylamide Hydrogel with <i>Lactococcus lactis</i> in it. hoping for growth and survival.
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Pouring a polyacrylamide hydrogel with <i>Lactococcus lactis</i> NZ9700 in it
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Before we continue with all the growth experiments we first want to check what percentage of gel works the best for our purposes.
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Four polyacrylamide gels with different concentration were poured, one of 10 %, 15 %, 20 %, 25 %, and 30 % acrylamide, we decided to continue with 20 % for now
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We will start the experiments with these percentages: 10 - 15 - 20 - 25 and 30 % acrylamid.
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For our purposes the 20% acrylamid gel works best, it doesn't break so fast and isn't that hard.
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After we decided the 20% gel works best we've decided to continue with the freeze drying and encapsulation of cells.
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Pouring a polyacrylamide gel containing an overnight culture, this gel is divided in four, two gels were freeze dried, and two more were incubated overnight at 30 °C with fresh M17 medium
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We've created 1 large 20% acrylamid gel which contains 1 ml of overnight culture.
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This gel is split up 4 ways. 2 of those we're freeze dried, and the other 2 we're put in the stove overnight (30 °C).
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These gels were checked on a phase contrast microscope the next day. It was clearly there were a lot of cells and that they were moving. We'll continue these experiments as soon as we have a GFP strain.
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These gels were checked by phase contrast microscopy after incubation overnight, a lot of cells were visible, but we could not distinguish the living of dead bacteria, therefore we decided to continue the experiment with an inducable GFP expressing strain
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Latest revision as of 01:24, 18 October 2014

July 21- July 25
 
Growing a new overnight cultures and preparing the chemicals for the first polyacrylamide gels with L. lactis cells
 
Pouring a polyacrylamide hydrogel with Lactococcus lactis NZ9700 in it
 
Four polyacrylamide gels with different concentration were poured, one of 10 %, 15 %, 20 %, 25 %, and 30 % acrylamide, we decided to continue with 20 % for now
 
Pouring a polyacrylamide gel containing an overnight culture, this gel is divided in four, two gels were freeze dried, and two more were incubated overnight at 30 °C with fresh M17 medium
 
These gels were checked by phase contrast microscopy after incubation overnight, a lot of cells were visible, but we could not distinguish the living of dead bacteria, therefore we decided to continue the experiment with an inducable GFP expressing strain
 
 
 
 

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