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Revision as of 09:17, 11 July 2014 (view source) Romain.sanson (Talk | contribs) (→Monday 30th June) ← Older edit		Revision as of 10:05, 11 July 2014 (view source) Romain.sanson (Talk | contribs) (→Monday 30th June) Newer edit →
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-	====4 - Preparations:====	+	====4 - Preparations :====
-	'''Marie, Mathieu & Romain'''	+	* Aliquots of antibiotics :
		+	'''Marie & Mathieu'''
		+	We weigh 0.203g of Chloramphenicol and put it in 10ml of ethanol.
		+	Then, we do aliquots of 1ml.
		+	We do the same for Kanamycin and Ampicillin.
-	* Aliquots of antibiotics
	* Petri dishes		* Petri dishes
		+	'''Romain'''
		+	We add 250µl of antibiotics in 250ml of LB

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Revision as of 10:05, 11 July 2014

Contents 1 Monday 30th June 1.1 Lab work 1.1.1 1 - Culture or the supercompetents: 1.1.2 2 - Réhydration of selected biobricks: 1.1.3 3 - Transformations : 1.1.4 4 - Preparations :

Monday 30th June

Lab work

1 - Culture or the supercompetents:

Mathias

• MG1655
• MG1655Z1

1mL of an overnight culture in 100mL of LB

2 - Réhydration of selected biobricks:

Juliette

• BBQ_I9026
• BBQ_K592025
• BBQ_K786002
• BBQ_K786003
• BBQ_K778002
• BBQ_K592011 {2 times}
• BBQ_K592009
• BBQ_K1033910 {idem}
• BBQ_J45014
• BBQ_K517003

3 - Transformations :

Maher & Mathias

We used the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.

4 - Preparations :

• Aliquots of antibiotics :

Marie & Mathieu We weigh 0.203g of Chloramphenicol and put it in 10ml of ethanol. Then, we do aliquots of 1ml. We do the same for Kanamycin and Ampicillin.

• Petri dishes

Romain We add 250µl of antibiotics in 250ml of LB

People there:

• Instructors : Solenne.
• Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

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