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Team:CAU China/Human Practice/Peers
From 2014.igem.org
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| - | We usually discussed projects, rules of iGEM competition and Synthetic Biology with NJAU_China , and gave some advice to them both on dry and wet experiments. When we knew NJAU_China needed to synthesize a 2.4kb gene, gshF, which would cost over RMB 7000 ($ 1200) through gene synthesis company, we provided a protocal which can direct people to synthsize gene with short single DNA oligotides (about 50-60 bp). The method mainly consists of single DNA oligotides designing and synthesizing by company, assembly, PCR amplification, transformation, check, sequencing and point mutaion ( sometimes necessary ). It takes similiar time length and 1/3-1/2 times less cost than synthesizing through company. | + | We usually discussed projects, rules of iGEM competition and Synthetic Biology with <p style="color:#000000"> <a href="https://igem.org/2014_Judging_Form?id=1555"style="color:#FFFFFF"> NJAU_China</a> , and gave some advice to them both on dry and wet experiments. When we knew <p style="color:#000000"> <a href="https://igem.org/2014_Judging_Form?id=1555"style="color:#FFFFFF"> NJAU_China</a> needed to synthesize a 2.4kb gene, gshF, which would cost over RMB 7000 ($ 1200) through gene synthesis company, we provided a protocal which can direct people to synthsize gene with short single DNA oligotides (about 50-60 bp). The method mainly consists of single DNA oligotides designing and synthesizing by company, assembly, PCR amplification, transformation, check, sequencing and point mutaion ( sometimes necessary ). It takes similiar time length and 1/3-1/2 times less cost than synthesizing through company. |
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Revision as of 08:40, 17 October 2014
Help PeersWe usually discussed projects, rules of iGEM competition and Synthetic Biology with NJAU_China , and gave some advice to them both on dry and wet experiments. When we knew NJAU_China needed to synthesize a 2.4kb gene, gshF, which would cost over RMB 7000 ($ 1200) through gene synthesis company, we provided a protocal which can direct people to synthsize gene with short single DNA oligotides (about 50-60 bp). The method mainly consists of single DNA oligotides designing and synthesizing by company, assembly, PCR amplification, transformation, check, sequencing and point mutaion ( sometimes necessary ). It takes similiar time length and 1/3-1/2 times less cost than synthesizing through company.
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