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Team:Northwestern/CFPS
From 2014.igem.org
(Difference between revisions)
| Line 69: | Line 69: | ||
<li>RBS B0034</li> | <li>RBS B0034</li> | ||
<li>Tried all of our lysates</li> | <li>Tried all of our lysates</li> | ||
| - | <li> | + | <li>green fluorescence indicates amount of transcription</li> |
| + | <li>red fluorescence indicates amount of translation</li> | ||
| + | <li><b>conclusion</b>: the data is most likely statistically meaningless</li> | ||
</ul> | </ul> | ||
Revision as of 04:42, 17 October 2014
Cell-Free Protein Synthesis
20140723 Testing our lysates
- data from BL21 (E.Coli) lysates
- out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
- the plasmid used was superfolder GFP from Jewett Lab
20140915 All of our lysates
- RBS B0034
- Tried all of our lysates
- green fluorescence indicates amount of transcription
- red fluorescence indicates amount of translation
- conclusion: the data is most likely statistically meaningless
