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Team:Carnegie Mellon/Superoxide
From 2014.igem.org
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<center><p><img src ="https://static.igem.org/mediawiki/2014/c/c5/800px-KillerRed-2.png" alt="Killer Red"</p></center> | <center><p><img src ="https://static.igem.org/mediawiki/2014/c/c5/800px-KillerRed-2.png" alt="Killer Red"</p></center> | ||
<center><p>iGEM Registry of Standard Biological Parts, Part:BBa K1184000</p></center> | <center><p>iGEM Registry of Standard Biological Parts, Part:BBa K1184000</p></center> | ||
| - | <p | + | <p> In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods<a href="https://2013.igem.org/Team:Carnegie_Mellon/Project/Abstract">(Source: Carnegie Mellon iGEM 2013)</a></p> |
<h4>Methods Used</h4> | <h4>Methods Used</h4> | ||
Revision as of 03:33, 17 October 2014
Superoxide Generator Improvement
iGEM Registry of Standard Biological Parts, Part:BBa K1184000
In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods(Source: Carnegie Mellon iGEM 2013)
Methods Used
- Optimization of codons in KillerRed by eliminating rare codon usage.
- Optimization of codons in Supernova, the monomeric version of KillerRed, by eliminating rare codon usage.
- Photobleaching analysis of KillerRed, Codon Optimized KillerRed, and Codon Optimized Supernova.
