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| - | <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
| + | /*This is wetlab2*/ |
| - | <html xmlns=" http://www.w3.org/1999/xhtml">
| + | |
| - | <head>
| + | |
| - | <meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
| + | |
| - | <title>LZU-China 2014</title>
| + | |
| - | <style type="text/css">
| + | |
| - | | + | |
| - | body,ul,li,ol{list-style:none;padding:0px;margin:0px;font-size:12px;font-family:微软雅黑;}
| + | |
| - | img{border:0px;}
| + | |
| - | .picList{width:100%;float:left;}
| + | |
| - | .picList .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList .col img{width:100%;}
| + | |
| - | .picList .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList2{width:100%;float:left;}
| + | |
| - | .picList2 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList2 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList2 .col img{width:100%;}
| + | |
| - | .picList2 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList3{width:100%;float:left;}
| + | |
| - | .picList3 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList3 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList3 .col img{width:100%;}
| + | |
| - | .picList3 .col a{
| + | |
| - | display:block;
| + | |
| - | width:580;
| + | |
| - | height:377px;
| + | |
| - | top:357px;
| + | |
| - | background:rgba(0,0,0,0.7);
| + | |
| - | color:white;
| + | |
| - | font-style:normal;
| + | |
| - | line-height:25px;
| + | |
| - | padding:10px;
| + | |
| - | opacity:0.8;
| + | |
| - | text-decoration:none;
| + | |
| - | }
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList4{width:100%;float:left;}
| + | |
| - | .picList4 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList4 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList4 .col img{width:100%;}
| + | |
| - | .picList4 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList5{width:100%;float:left;}
| + | |
| - | .picList5 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList5 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList5 .col img{width:100%;}
| + | |
| - | .picList5 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList6{width:100%;float:left;}
| + | |
| - | .picList6 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList6 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList6 .col img{width:100%;}
| + | |
| - | .picList6 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList7{width:100%;float:left;}
| + | |
| - | .picList7 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList7 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList7 .col img{width:100%;}
| + | |
| - | .picList7 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | .picList8{width:100%;float:left;}
| + | |
| - | .picList8 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
| + | |
| - | .picList8 .col img,.col a{position:absolute;top:0px;left:0px;}
| + | |
| - | .picList8 .col img{width:100%;}
| + | |
| - | .picList8 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
| + | |
| - | .msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
| + | |
| - | | + | |
| - | *{margin:0px;padding:0px;} | + | |
| - | body{background:#b1b1b1;margin:0px;padding:0px;font-size:14px;color:#000;}
| + | |
| - | b#p1, b#p2, b#p3, b#p4, b#p5 {display:none;}
| + | |
| - | #accordian {padding:0; margin:20px auto; list-style:none; width:730px; height:300px; overflow:hidden; position:relative; border:1px solid #682;}
| + | |
| - | #accordian li {width:50px; height:300px; float:left;
| + | |
| - | -moz-transition: 0.7s;
| + | |
| - | -ms-transition: 0.7s;
| + | |
| - | -o-transition: 0.7s;
| + | |
| - | -webkit-transition: 0.7s;
| + | |
| - | transition: 0.7s;
| + | |
| - | }
| + | |
| - | #accordian div {width:530px; height:300px; position:relative;}
| + | |
| - | #accordian div span {display:block; background:rgba(0,0,0,.3); width:460px; height:30px; position:absolute; bottom:300px; right:0; padding:10px; color:#fff; font:normal 13px/30px 'georgia', serif; border-top:1px solid #000;
| + | |
| - | }
| + | |
| - | #accordian div span a {color:#fff;}
| + | |
| - | #accordian li div > a {display:block; width:49px; height:300px; float:left; position:relative; color:#fff; text-decoration:none;box-shadow:-10px 0 10px -10px rgba(0,0,0,0.6);}
| + | |
| - | #accordian li div > a b {display:block; width:280px; padding-left:20px; height:50px; position:absolute; left:50px; bottom:0; font:bold 24px/50px 'georgia', serif; text-shadow:0 0 2px rgba(0, 0, 0, 0.6);
| + | |
| - | transform: rotate(-90deg);
| + | |
| - | transform-origin:bottom left;
| + | |
| - | -ms-transform: rotate(-90deg);
| + | |
| - | -ms-transform-origin:bottom left;
| + | |
| - | -webkit-transform: rotate(-90deg);
| + | |
| - | -webkit-transform-origin:bottom left;
| + | |
| - | -moz-transform: rotate(-90deg);
| + | |
| - | -moz-transform-origin:bottom left;
| + | |
| - | -o-transform: rotate(-90deg);
| + | |
| - | -o-transform-origin:bottom left;
| + | |
| - | }
| + | |
| - | #accordian li div > a b {*left:0;}
| + | |
| - | #accordian li img {display:block; float:left;}
| + | |
| - | #accordian li.p1 {width:530px;}
| + | |
| - | #accordian li.p1 div span {bottom:0;}
| + | |
| - | #accordian li a.a1 {background:#b33866; border-left:1px solid #682;}
| + | |
| - | #accordian li a.a2 {background:#8d4984; border-left:1px solid #9b5;}
| + | |
| - | #accordian li a.a3 {background:#6559a1; border-left:1px solid #ce5;}
| + | |
| - | #accordian li a.a4 {background:#3c6cc0; border-left:1px solid #ef7;}
| + | |
| - | #accordian li a.a5 {background:#187adb; border-left:1px solid #28b;}
| + | |
| - | #p1:target ~ #accordian li {width:50px;}
| + | |
| - | #p1:target ~ #accordian li div span {bottom:300px;}
| + | |
| - | #p1:target ~ #accordian li.p1 {width:530px;}
| + | |
| - | #p1:target ~ #accordian li.p1 div span {bottom:0;}
| + | |
| - | #p2:target ~ #accordian li {width:50px;}
| + | |
| - | #p2:target ~ #accordian li div span {bottom:300px;}
| + | |
| - | #p2:target ~ #accordian li.p2 {width:530px;}
| + | |
| - | #p2:target ~ #accordian li.p2 div span {bottom:0;}
| + | |
| - | #p3:target ~ #accordian li {width:50px;}
| + | |
| - | #p3:target ~ #accordian li div span {bottom:300px;}
| + | |
| - | #p3:target ~ #accordian li.p3 {width:530px;}
| + | |
| - | #p3:target ~ #accordian li.p3 div span {bottom:0;}
| + | |
| - | #p4:target ~ #accordian li {width:50px;}
| + | |
| - | #p4:target ~ #accordian li div span {bottom:300px;}
| + | |
| - | #p4:target ~ #accordian li.p4 {width:530px;}
| + | |
| - | #p4:target ~ #accordian li.p4 div span {bottom:0;}
| + | |
| - | #p5:target ~ #accordian li {width:50px;}
| + | |
| - | #p5:target ~ #accordian li div span {bottom:300px;}
| + | |
| - | #p5:target ~ #accordian li.p5 {width:530px;}
| + | |
| - | #p5:target ~ #accordian li.p5 div span {bottom:0;}
| + | |
| - | | + | |
| - | | + | |
| - | #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
| + | |
| - | | + | |
| - | .firstHeading {
| + | |
| - | width: 975px;
| + | |
| - | margin: 0px auto;
| + | |
| - | padding-top: 100px;
| + | |
| - | margin-bottom: 20px;
| + | |
| - | font-family: Georgia, Times, "Times New Roman", serif;
| + | |
| - | }
| + | |
| - | | + | |
| - | h1, h2, h3, h4, h5 { font-family: Georgia, Times, "Times New Roman", serif;}
| + | |
| - | | + | |
| - | #top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
| + | |
| - | background-color: #383838;
| + | |
| - | border: 0 none;
| + | |
| - | height: 14px;
| + | |
| - | z-index: 100;
| + | |
| - | top: 0;
| + | |
| - | position: fixed;
| + | |
| - | width: 975px;
| + | |
| - | left: 50%;
| + | |
| - | margin-left: -487px;
| + | |
| - | }
| + | |
| - | | + | |
| - | #top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
| + | |
| - | background-color: #383838;
| + | |
| - | height: 14px;
| + | |
| - | display: block;
| + | |
| - | z-index: 10;
| + | |
| - | position: fixed;
| + | |
| - | width: 100%;
| + | |
| - | top: 0;
| + | |
| - | }
| + | |
| - | | + | |
| - | #menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
| + | |
| - | color: #727272;
| + | |
| - | text-decoration: none;
| + | |
| - | background-color: transparent;
| + | |
| - | }
| + | |
| - | | + | |
| - | body {
| + | |
| - | background-color: #fff;
| + | |
| - | }
| + | |
| - | | + | |
| - | #globalWrapper, #content { /*-- changes default wiki settings --*/
| + | |
| - | width: 100%;
| + | |
| - | height: 100%;
| + | |
| - | border: 0px;
| + | |
| - | background-color: transparent;
| + | |
| - | margin: 0px;
| + | |
| - | padding: 0px;
| + | |
| - | }
| + | |
| - | | + | |
| - | html, body, .wrapper { /*-- changes default wiki settings --*/
| + | |
| - | width: 100%;
| + | |
| - | height: 100%;
| + | |
| - | background-color: transparent;
| + | |
| - | }
| + | |
| - | | + | |
| - | #contentcontainer { /*-- creates container for all content on page --*/
| + | |
| - | font-family: Arial, Helvetica, sans-serif;
| + | |
| - | font-weight: normal;
| + | |
| - | font-size: 14px;
| + | |
| - | color: #414141;
| + | |
| - | width: 960px;
| + | |
| - | margin-left: auto;
| + | |
| - | margin-right: auto;
| + | |
| - | background-color: #transparent;
| + | |
| - | margin-top: 0px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .sidemenu, .sidemenu li {
| + | |
| - | list-style-type: none;
| + | |
| - | list-style-image: none;
| + | |
| - | font-family: verdana;
| + | |
| - | text-decoration: none;
| + | |
| - | color:#000;
| + | |
| - | font-size: 14px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .sidemenu li {display: block;}
| + | |
| - | | + | |
| - | .sidemenu a {
| + | |
| - | text-decoration:none;
| + | |
| - | color: #000;
| + | |
| - | display: block;
| + | |
| - | width: 180px;
| + | |
| - | height: 100%;
| + | |
| - | padding: 3px 5px;
| + | |
| - | transition: .25s ease-in-out;
| + | |
| - | -moz-transition: .25s ease-in-out;
| + | |
| - | -webkit-transition: .25s ease-in-out;
| + | |
| - | }
| + | |
| - | | + | |
| - | .sidemenu a.greyout { color: #909090; }
| + | |
| - | | + | |
| - | .sidemenu a:hover {
| + | |
| - | color:#666;
| + | |
| - | background-color: #d9f5aa;
| + | |
| - | }
| + | |
| - | | + | |
| - | .sub-sidemenu {font-size: 10px; margin-bottom: 20px; color: #666; display: none;}
| + | |
| - | .sub-sidemenu a {color: #666;}
| + | |
| - | | + | |
| - | div.underconst
| + | |
| - | {
| + | |
| - | padding:10px;
| + | |
| - | border-radius:5px;
| + | |
| - | background-color:pink;
| + | |
| - | text-align:center;
| + | |
| - | }
| + | |
| - | | + | |
| - | a.anchor{display: block; position: relative; top: -80px; visibility: hidden;}
| + | |
| - | | + | |
| - | .contentpara {margin-bottom: 30px;}
| + | |
| - | | + | |
| - | span.email{font-family: monospace; font-weight: normal;}
| + | |
| - | | + | |
| - | fieldset {border: 1px solid #337f53;}
| + | |
| - | | + | |
| - | .greyout {color: #A0A0A0;}
| + | |
| - | .highlightme {background-color: #FFFF00;}
| + | |
| - | | + | |
| - | #alertContainer {border: 2px solid red; margin-bottom: 10px; width: 936px;}
| + | |
| - | #annContainer {margin-left: 8px;}
| + | |
| - | #newsContainer {border: 1px solid #ccc;}
| + | |
| - | | + | |
| - | .newsTitle {
| + | |
| - | display: block;
| + | |
| - | color: #414141;
| + | |
| - | font-size: 25px;
| + | |
| - | font-family: Georgia, Times, "Times New Roman", serif;
| + | |
| - | padding: 10px 15px 5px 10px;
| + | |
| - | border-bottom: 1px solid #ccc;
| + | |
| - | margin-bottom: 0px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .newsItem {
| + | |
| - | border-bottom: 1px solid #ccc;
| + | |
| - | display: block;
| + | |
| - | padding: 5px 15px 0px 10px;
| + | |
| - | margin-bottom: 0px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .newsItem h3 {
| + | |
| - | width: auto;
| + | |
| - | display: inline;
| + | |
| - | font-size: 14px;
| + | |
| - | font-family: Arial, Helvetica, sans-serif;
| + | |
| - | padding: 0px;
| + | |
| - | margin: 0px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .newsItem img {
| + | |
| - | float: right;
| + | |
| - | clear: right;
| + | |
| - | width: 80px;
| + | |
| - | padding-left: 15px;
| + | |
| - | display: inline-block;
| + | |
| - | }
| + | |
| - | | + | |
| - | .newsItem .newsDate {
| + | |
| - | font-style: italic;
| + | |
| - | font-size: 14px;
| + | |
| - | display: inline-block;
| + | |
| - | color: #999;
| + | |
| - | float: right;
| + | |
| - | padding: 0px 0px 10px 15px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .newsItem p, .newsItem ul, .newsItem li {
| + | |
| - | padding: 0px;
| + | |
| - | margin-left: 20px;
| + | |
| - | font-size: 12px;
| + | |
| - | line-height: 1.2;
| + | |
| - | }
| + | |
| - | | + | |
| - | .newsItem p {margin-top: 10px;}
| + | |
| - | | + | |
| - | .annItem {
| + | |
| - | border: 2px solid #49b677;
| + | |
| - | display: block;
| + | |
| - | padding: 0px 15px 10px 10px;
| + | |
| - | margin-bottom: 18px;
| + | |
| - | position: relative;
| + | |
| - | height: 160px;
| + | |
| - | vertical-align: middle;
| + | |
| - | }
| + | |
| - | | + | |
| - | .annItem .annCentered {
| + | |
| - | display: table-cell;
| + | |
| - | vertical-align: middle;
| + | |
| - | height: 160px;
| + | |
| - | }
| + | |
| - | | + | |
| - | .annCentered h3 {
| + | |
| - | padding: 0px;
| + | |
| - | display: block;
| + | |
| - | margin-left: auto;
| + | |
| - | margin-right: auto;
| + | |
| - | text-align: center;
| + | |
| - | }
| + | |
| - | | + | |
| - | .annItem ul, .annItem li {padding: 0px; margin: 0px;}
| + | |
| - | .annItem li {list-style: none; margin: 5px}
| + | |
| - | | + | |
| - | @charset "utf-8";
| + | |
| - | body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
| + | |
| - | margin:0;
| + | |
| - | padding:0;
| + | |
| - | font-size: 14px;
| + | |
| - | text-align: left;
| + | |
| - | }
| + | |
| - | html{background-color:#e9ecec}
| + | |
| - | table{table-layout:fixed;border-collapse:collapse}
| + | |
| - | section,article,aside,header,footer,nav,dialog,figure{display:block}
| + | |
| - | .clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both}
| + | |
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| - | <table width="100%" border="0" >
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| - | <td width="1201" height="265" rowspan="2"><p><img src="https://static.igem.org/mediawiki/2014/thumb/9/99/9.notebook.top.jpg/800px-9.notebook.top.jpg" width="1366" height="270" /></p></td>
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| - | </tr>
| + | |
| - | <tr>
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| - | <td height="70"> </td>
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| - | </tr>
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| - | </div>
| + | |
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| - | <table width="100%" border="0">
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| - | <tr>
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| - | <td width="25%"> </td>
| + | |
| - | <td><p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> "Rome was not built in a day." No matter which group we belong to, we took our work for almost a whole year. What did we do on earth? This notebook will show you in detail.</p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <div><a href="https://2014.igem.org/Team:LZU-China/Notebook"><img src="https://static.igem.org/mediawiki/2014/8/8f/Z.wabb%281%29.png" width="214" height="99" /></a> <a href="https://2014.igem.org/Team:LZU-China/NotebookB"><img src="https://static.igem.org/mediawiki/2014/e/e6/Z.wabb%282%29.png" width="215" height="99" /></a><a href="https://2014.igem.org/Team:LZU-China/NotebookC"><img src="https://static.igem.org/mediawiki/2014/0/0e/Z.wabb%283%29.png" width="212" height="99" /></a></div>
| + | |
| - | <p> </p></td>
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| - | <td width="25%"> </td>
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| - | </tr>
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| - | </table>
| + | |
| - | <p> </p>
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| - | </div>
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| - | <p> </p>
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| - | </tr>
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| - | <tr>
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| - | <td width="20%"> </td>
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| - | </tr>
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| - | </table>
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| - | </div>
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| - | <div id="apDiv5">
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| - | <table width="1365" border="0"">
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| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="1365" height="463"><img src="https://static.igem.org/mediawiki/2014/0/07/Dblbluebar.jpg" width="1365" height="463" /></td>
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| - | </tr>
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| - | </table>
| + | |
| - | </div>
| + | |
| - | <div id="apDiv6"><img src="https://static.igem.org/mediawiki/2014/6/6e/Lzulogo.png" width="220" height="221" /></div>
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| - | <div id="apDiv8">
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| - | <p> </p>
| + | |
| - | <table width="100%" border="0">
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| - | <tr>
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| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg"><p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <table width="100%" border="0" >
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| - | <tr>
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| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" > THE NOTEBOOK OF WET LAB GROUP A</td>
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| - | </tr>
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| - | </table></td>
| + | |
| - | </tr>
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| - | <tr>
| + | |
| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><table width="100%" border="0">
| + | |
| - | <tr>
| + | |
| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="30%"> </td>
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| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="57%"><p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2014.5.16-5.23<br />
| + | |
| - | The pre-experience of the growth of three strains(<em>Shewanella onedensis,</em> <em>Pseudomonas aeruginosa </em>and LZU-3, which is separated in Li’s lab )in different PNP concentration and Cr(VI) concentration.</p>
| + | |
| - | <p> </p>
| + | |
| - | <p> <img src="https://static.igem.org/mediawiki/2014/f/f5/Z.da1.png" width="700" height="173" /></p>
| + | |
| - | <div><img src="file:///E|/[iGEM]/wiki/2014logomin.png" width="358" height="40" /></div>
| + | |
| - | <p> </p>
| + | |
| - | <p> <img src="file:///E|/https://static.igem.org/mediawiki/2014/e/e0/Partpic2.png" width="301" height="251" /></p>
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| - | <div><img src="file:///E|/[iGEM]/wiki/partpic2.png" width="301" height="251" /></div>
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| - | <p> </p>
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| - | <p> </p>
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| - | <p> 2014.5.24-5.31<br />
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| - | Measurement for three strains’(<em>Shewanella onedensis,</em> <em>Pseudomonas aeruginosa </em>and LZU-3, which is separated in Li’s lab ) Cr(VI) reduction activity.</p>
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| - | <div><img src="file:///E|/[iGEM]/wiki/dblbluebar.jpg" alt="" width="1365" height="463" /></div>
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| - | <p> </p>
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| - | <p> <img src="https://static.igem.org/mediawiki/2014/thumb/d/dc/Z.a2.png/463px-Z.a2.png" width="467" height="605" /></p>
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| - | <p> </p>
| + | |
| - | <p> 2014.6.1-2014.6.8<br />
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| - | 1.Another measurement for three strains’(as above said) Cr(VI) reduction activity.</p>
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| - | <p> <img src="https://static.igem.org/mediawiki/2014/a/a6/Z.a3.png" width="569" height="482" /></p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2.Construction of the phylogeny tree containing the LZU-3 </p>
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| - | <p> <img src="https://static.igem.org/mediawiki/2014/5/52/Z.4.png" width="654" height="503" /></p>
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| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2014.6.8-2014.7.24<br />
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| - | The PCR cycling for BBa_I723020 and BBa_I723133</p>
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| - | <p> </p>
| + | |
| - | <p> 1. The primers(synthesized by BGI,shenzhen)</p>
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| - | <p> 1.1 Promoter of BBa_I723133 <br />
| + | |
| - | The forward primer (5’ TATACTAGTCTGGGGCGAGAGGCGACGAC 3’) contained a SpeI cutsite <br />
| + | |
| - | The reverse primer (5’ GGGGAATTCTAGAATGTGGGCTGCTTGGTG 3’) contained a EcoRI and XbaI cutsite. <br />
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| - | 2313bp</p>
| + | |
| - | <p> 1.2 BBa_I723020<br />
| + | |
| - | The forward primer (5’GGTGCTGCAGACTAGTATTGAAGGGTCACCACTATTTTTATTTTA 3’) containes SpeI and PstI cutsites <br />
| + | |
| - | the reverse primer (5’ ATGAATTCTCTAGACCTGCTGGAGGGCGTGAAC 3’) containes EcoRI and XbaI cutsites. <br />
| + | |
| - | 340bp</p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2.The PCR system</p>
| + | |
| - | <p> 0.2μL ExTaq.<br />
| + | |
| - | 1μL bacterial liquid<br />
| + | |
| - | 1μL dNTP<br />
| + | |
| - | 1μL forward primer<br />
| + | |
| - | 1μL reverse primer<br />
| + | |
| - | 2.5μL 10x Buffer<br />
| + | |
| - | 18.5μL ddH2O</p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2014.7.31-8.1<br />
| + | |
| - | Pre-experience for the function of BBa_K381001.<br />
| + | |
| - | BBa_K381001 transformation to E.coli DH5 alpha<br />
| + | |
| - | There are two groups to test the green fluorescence under microscope:<br />
| + | |
| - | (These groups' total sizes are all 20μL, had been shaken for 2hours before observation,400x)<br />
| + | |
| - | a.bacterial liquid with 10mM PNP concentration.<br />
| + | |
| - | b.bacterial liquid with 20mM PNP concentration.<br />
| + | |
| - | </p>
| + | |
| - | <p> <img src="https://static.igem.org/mediawiki/2014/2/2d/Z.5.png" width="704" height="307" /></p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2014.8.2-2014.8.6<br />
| + | |
| - | Pre-experience for the function of BBa_K381001.<br />
| + | |
| - | BBa_K381001 transformation to E.coli DH5 alpha<br />
| + | |
| - | There are four groups to test the green fluorescence under microscope:<br />
| + | |
| - | (These groups' total sizes are all 20μL, had been shaken for 2hours before observation,1000x)<br />
| + | |
| - | a.bacterial liquid with 10mM PNP concentration.b.<br />
| + | |
| - | b.bacterial liquid with ddH2O<br />
| + | |
| - | c.bacterial liquid with 10mM KNO3<br />
| + | |
| - | d.bacterial liquid with 10mM KCl.<br />
| + | |
| - | </p>
| + | |
| - | <p> <img src="https://static.igem.org/mediawiki/2014/4/41/Z.a6.png" width="455" height="340" /></p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2014.8.25-9.21<br />
| + | |
| - | 1.Construction of BBa_K1523101<br />
| + | |
| - | </p>
| + | |
| - | <p> <img src="https://static.igem.org/mediawiki/2014/2/28/Z.a7.png" width="489" height="90" /></p>
| + | |
| - | <p> </p>
| + | |
| - | <p> The size of the whole part is about 3700bp(without plasmid), we test the assembling result by PCR(sense:5'---TTCCCATCTATAATCCTCCCTGATTCTTCG---3';anti-sense:5'---GAATTCTCTAGATTACAACTGTTGTTCAAGCTGTT---3'). From the gel picture we can see the size is right.</p>
| + | |
| - | <p> <img src="https://static.igem.org/mediawiki/2014/c/cf/Z.a8.png" width="643" height="305" /></p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2.Construction of BBa_K1523024(yieF) and BBa_K1523005(chrR)</p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> 2014.9.21-10.2<br />
| + | |
| - | Construction of BBa_K1523021(<em>nahR</em>), K1523022(<em>nahE</em>) and K1523023(<em>nahG</em>).</p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p> </p>
| + | |
| - | <p>
| + | |
| - | <p>
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| - | <p>
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| - | <p>
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| - | <p>
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| - | <p>
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| - | <p> </td>
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| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="13%"> </td>
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| - | </tr>
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| - | </table></td>
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| - | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><p> </p>
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| - | <p> </p></td>
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