Team:Austin Texas/interlab study
From 2014.igem.org
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*The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. Triplicate cultures were continued. | *The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. Triplicate cultures were continued. | ||
*After 16-18 hours, add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | *After 16-18 hours, add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | ||
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+ | The above protocol was described in more detail on the [https://2014.igem.org/Tracks/Measurement/Interlab_study interlab study page]. | ||
<h3>Measurement Protocol</h3> | <h3>Measurement Protocol</h3> |
Revision as of 03:28, 17 October 2014
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