Virtus-Parva During the length of our project, we found that importing chemicals and biological agents that we needed were difficult to pass through customs in Mexico. In coordination with other Mexican teams, specially [https://2014.igem.org/Team:Tec-Monterrey/ITESM14_human_practice.html#tab_law-proposal "Tec-Monterrey team"] , we decided to propose to the senate a change in customs law, to make it easier for future iGEMers or other scientists to continue with their investigations. of Nanotechnology students who aim to create a better world through love,sympathy and endearment… and through the design and development of a novel technology based on micrometric “drills” to attack pathogen agents.

First and foremost, we studied different procedures in order to synthesize the strongest and smallest magnetite particles we could make. Magnetite is a molecule that derives from iron, and has observable magnetic properties. Because we wanted to put together magnetite and DNA, we had to make them compatible, task we accomplished by functionalizing it with amino groups, that would allow it to form peptide bonds with our protein, HU. At the same time, our biology team was busy extracting, purifying and transforming E. Coli DNA to work with. This protein, HU, is a histone-like protein normally aids DNA into supercoiling around histones; the “Magnetic-Protein” complex we created mimics the nucleosome in DNA supercoiling process. This allows us to have a DNA “chromatin” with a magnetic core.

Combining magnetite, DNA and HU protein, we are building magnetic-core machines, which can be controlled through external electrical impulses. Because of the shape of our system, it is possible for it to have linear movement depending on the frequency applied to it, which has the potential to be incorporated into the medical sector as a pathogen-targeted therapy. This was our original idea and module one of our project.

As we were transforming our E. coli cells, we noticed it wasn’t as fast and efficient as we had hoped, which is how we came up with module two of the project. Quite simply, we wanted to take advantage of the shape of our system and its mobility thanks to magnetism in order to make a more efficient transformation. We were able to verify our method was more efficient by making cells express GFP and RFP, which can then be quantified with optic instruments.

NEMS are nanometric electromechanical systems. In this case we take as basis the structure of a resonator which are engineered to make a conversión between energy, such as electric, magnetic, or vibrational into mechanical response.

How exactly do NEMS come into play in our project?

Well, by combining an inorganically synthesized nanoparticle, called magnetite and DNA into what we call BioNEMS drill.

Contenido subtitulo 2

The first part of the synthesis of our magnetite was trying out different methods and characterizing them, to note which method had given us the smallest size nanoparticles. Our first method was synthesis by coprecipitation, of which we prepared nine samples with different concentrations of iron(II) chloride and ammonium hydroxide; from this method we consistently obtained nanoparticles rounding 0.9 to 1nm. Our following method was very similar, but included water in the synthesis: the size of our particles would vary greatly, from 3.89 micrometers to 171 nanometers in size.

After choosing the best method possible, it was time to silanize our magnetite in order for it to be biocompatible with DNA and be able to tie them together. In order for the silanization to take place, we used a solution of TEOS (tetraethoxysilane) dispersed in a medium of water and propanol and dripped this mix slowly onto our magnetite. Just like when we synthesized our particles, we tested different concentrations of TEOS and magnetite, as well as different addition rates in order to observe which combination would give us the smallest possible nanoparticles.

Our results were then characterized by DLS (dynamic light scattering), for which we observed a peak at 39 nm, once coated with TEOS, the peak was moved toward 60 and 80 nm. We also ran our two samples in the IR, comparing the spectra of the pure magnetite and silanized magnetite, we were able to distinguish a peak at 990.2 cm^-1 corresponding to a Si-O bond, confirming the correct silanization of the magnetite.

Soon..