Team:Austin Texas/photocage
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=Experimental Methods= | =Experimental Methods= | ||
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To demonstrate the function of this system, cells were grown in culture... These cells were then exposed to varying amounts of 365 nm light, ranging from 0 to 30 minutes. During this exposure, the ONB functional group at position ONBY639 should be released, resulting in Y639. | To demonstrate the function of this system, cells were grown in culture... These cells were then exposed to varying amounts of 365 nm light, ranging from 0 to 30 minutes. During this exposure, the ONB functional group at position ONBY639 should be released, resulting in Y639. | ||
- | After this exposure, the cells were grown for an additional | + | After this exposure, the cells were grown for an additional [1 hour?], allowing the newly decaged T7 RNAP to transcribe the GFP reporter gene, which ultimately results in fluorescence. |
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+ | [[File:ONBY PQE photocage 10-15-14.png| 250px|thumb|right|Spacio-temporal control over GFP translation.]] | ||
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=Discussion= | =Discussion= |
Revision as of 01:43, 17 October 2014
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