Team:Sumbawagen/Notebook

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<h1 class="firstHeading">Team:Sumbawagen/Team</h1>
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<h3 id="siteSub" class='noprint'>From 2014.igem.org</h3>
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    <title>iGEM Sumbawagen 2014 &middot; Econey</title>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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            <a class="brand" href="https://2014.igem.org/Team:Sumbawagen">Sumbawagen</a>
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<a href="https://2014.igem.org/Team:Sumbawagen/Project"style="color:#000000"> Project</a></td>
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                <li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li>
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<a href="https://2014.igem.org/Team:Sumbawagen/Parts"style="color:#000000"> Parts</a></td>
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
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                      <li><a href="#"> Sub Menu 1 </a></li>
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                      <!--<li><a href="#">Sub Menu 2 </a></li>-->
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                      <li><a href="https://2014.igem.org/Team:Sumbawagen/Modeling">Modelling </a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/humanpractice_overview">Human Practice </a></li>
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<a href="https://2014.igem.org/Team:Sumbawagen/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:Sumbawagen/Notebook"style="color:#000000"> Notebook</a></td>
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                <li class="dropdown">
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                  <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
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                    <li class="nav-header">Lab</li>
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                  <!-- <li><a href="#">Mop</a></li>-->
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                    <li><a href="#">Sub Menu <i>Miring</i> </a></li>
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                    <li class="nav-header">Modelling </li>
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                    <li class="nav-header">Software &amp; Hardware</li>
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                  <li><a href="#">Glucose Measurement</a></li>
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                    <li class="nav-header">Attributions </li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attributions </a></li>
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                </li>
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                <li class="dropdown">
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                    <li><a href="#">Our Biobricks</a></li>
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                <li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
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                    <li><a href="http:https://igem.org/Team.cgi">Team Information</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Notebook</a></li>
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                    <li class="nav-header">Social Media</li>
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  <sup>*New</sup></div>
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<li><a href="#">Media Coverage</a></li>
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                    <li><a href="#">Political Campaign</a></li>
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<li><a href="#">Media Coverage</a></li>
 +
                    <li><a href="#">Clatto Case Study</a></li>
 +
                    <li><a href="#">Public Engagement</a></li>
 +
                    <!--<li><a href="#">Interviews</a></li>-->
 +
                    <li><a href="#">Collaboration</a></li>
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                    <li class="divider"></li>
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                    <li class="nav-header">Media</li>
 +
                    <li><a href="http://www.youtube.com/channel/UCvHOQ9Y1PqKInj6iCwLqTJw/feed?view_as=public">Youtube Channel</a></li>
 +
                    <li><a href="http://www.flickr.com/photos/97927329@N05/">Flickr</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Human Practice/comic">Comic</a></li>
 +
                    <li><a href="#">Video Game</a></li>
 +
<li><a href="#">Digital Poster</a></li>
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<!--requirements section -->
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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
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                  </ul>
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                <li class="dropdown">
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study <b class="caret"></b></a>
 +
                  <ul class="dropdown-menu">
 +
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/interlabstudy/results">Results</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/interlabstudy/Notebook">Notebook</a></li>
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                  </ul>
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
 
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      <div class="container">
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      <!-- Title -->
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      <div class="page-header">
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          <h2><b>Week 1</b> </h2>
 +
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      <!-- Title End -->
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      <div class="row">
 +
      <!-- the main content -->
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 +
        <div class="span12" style="text-align:justify;margin-top:-30px;">
 +
<p>We began our project by preparing material needed. We made liquid and solid medium using LB powder. We also made solution for transformation process like MgCl2, and TSS medium. In this week, we also culture the stock of our RFP in liquid medium. We aim to make the stock of RFP and also examine whether the RFP still work or not. The culture of RFP is not growth. It indicates that our RFP stock has been damaged due to the unstable storage temperature.  </p>
 +
 +
<h2>Week 2</h2>
 +
<p>In the second week we culture the stock of RFP into the solid medium. The purpose is to make the stock of RFP in the solid medium. We also transformed the plasmid Bba_J04450 and Bba_B0034 into E coli BL21 (DE3). We did transformation twice in this week. The first transformation does not show any red colony growing. But the second transformation showed red colonies growing indicates that the transformation process success.<br><br>
 +
 +
<strong>Detail Activity : </strong>
 +
<p>1. August 18th, 2014</p>
 +
Making solid medium with chlorampenicol, take Bba_B0034 from kit plate and storage
 +
<p>2. August 19th, 2014</p>
 +
Making ampicilin stock 1 M, streak RFP (stock lab) medium padat, transformation
 +
<p>3. August 20th, 2014</p>
 +
Check Bba_J04450 transformation result, culture RFP, streak RFP, making ampicilin stock 1 M, plating transformation result, streak RFP from solid medium
 +
<p>4. August 21th, 2014</p>
 +
Check transformation results (Not growing), check RFP streak (contaminant), streak transformation result
 +
<p>5. August 22th, 2014</p>
 +
Culture E coli glycerol stock (BL21(DE3)), Transformation
 +
</p>
 +
<center><img src="https://static.igem.org/mediawiki/2013/1/1e/Somethingbeautiful.png"></img></center>
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          <h2>Week 3</h2>
 +
          <P><strong> Detail Activity </strong></p>
 +
<p>1. 25 August, 2014</p>
 +
Culture RFP DH5α (Add over expression reagent), making chlorampenicol stock
 +
<p>2. 26 August, 2014</p>
 +
Culture E coli glycerol stock RFP, culture over expression DH5α in 6 tube, streak RFP from single colony and line colony to the LB medium with and without chlorampenicol
 +
<p>3. 27 August, 2014</p>
 +
Plating transformation results, streak stock RFP
 +
 +
       
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    </div>
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<!-- Title -->
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      <div class="page-header">
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          <center><strong><h2><b>Sponsors</b> </h2></strong></center>
 +
<center><img src="https://static.igem.org/mediawiki/2014/3/30/Logo_Alzyme.jpg"><br></center>
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<center><img src="https://static.igem.org/mediawiki/2014/1/17/Logo-UTS.png"><br></center>
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Revision as of 01:59, 8 September 2014

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Sumbawagen

Week 1

We began our project by preparing material needed. We made liquid and solid medium using LB powder. We also made solution for transformation process like MgCl2, and TSS medium. In this week, we also culture the stock of our RFP in liquid medium. We aim to make the stock of RFP and also examine whether the RFP still work or not. The culture of RFP is not growth. It indicates that our RFP stock has been damaged due to the unstable storage temperature.

Week 2

In the second week we culture the stock of RFP into the solid medium. The purpose is to make the stock of RFP in the solid medium. We also transformed the plasmid Bba_J04450 and Bba_B0034 into E coli BL21 (DE3). We did transformation twice in this week. The first transformation does not show any red colony growing. But the second transformation showed red colonies growing indicates that the transformation process success.

Detail Activity :

1. August 18th, 2014

Making solid medium with chlorampenicol, take Bba_B0034 from kit plate and storage

2. August 19th, 2014

Making ampicilin stock 1 M, streak RFP (stock lab) medium padat, transformation

3. August 20th, 2014

Check Bba_J04450 transformation result, culture RFP, streak RFP, making ampicilin stock 1 M, plating transformation result, streak RFP from solid medium

4. August 21th, 2014

Check transformation results (Not growing), check RFP streak (contaminant), streak transformation result

5. August 22th, 2014

Culture E coli glycerol stock (BL21(DE3)), Transformation

Week 3

Detail Activity

1. 25 August, 2014

Culture RFP DH5α (Add over expression reagent), making chlorampenicol stock

2. 26 August, 2014

Culture E coli glycerol stock RFP, culture over expression DH5α in 6 tube, streak RFP from single colony and line colony to the LB medium with and without chlorampenicol

3. 27 August, 2014

Plating transformation results, streak stock RFP

Sponsors




Retrieved from "https://2013.igem.org/Team:Dundee"

Retrieved from "http://2014.igem.org/Team:Sumbawagen/Notebook"