Team:Clemson

From 2014.igem.org

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<h2>Project Overview</h2>
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<p style="color:black">This year, the Lethbridge iGEM team is working to bring a whole new class of parts to the iGEM community: programmed ribosomal frameshifting elements. To do this, we have been working towards standardizing the PK401 pseudoknot for use within the BioBrick system. These RNA secondary structural elements cause the ribosome to switch between translational frames and give another degree of freedom when engineering genetic circuits.</p><br>
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<h1 >Clemson iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Clemson&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<b style="color:black"><u>WHAT?</u></b>
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<ul style="color:black"><li>Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community</li></ul>  
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<b style="color:black"><u>WHY?</u></b>
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<ul style="color:black"><li>As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications</li></ul>
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<b style="color:black"><u>HOW?</b></u>
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<ul style="color:black"><li>We have brought pseudoknots to the iGEM community by:
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<ul><li>Characterizing their function in a biobrick system</li>
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<li>Designing software that enables others to dual code proteins</li>
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<li>Ensuring that the release of these tools to the wider public does not pose a significant risk to the rest of the world</li></li></ul></ul><br>
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<h2>Sponsors</h2>
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<a href="https://2014.igem.org/Team:Clemson"style="color:#000000">Home </a> </td>
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<br>
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<p><center><image src="https://static.igem.org/mediawiki/2013/0/00/ULeth2013_Sponsors_-_Platinum.png"; width="200px"; height="100px" />&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2014.igem.org/Team:Clemson/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Clemson"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Clemson/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Clemson/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Clemson/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Clemson/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:Clemson/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Clemson/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:Clemson">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Clemson">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:Clemson/Attributions">Attributions</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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=Welcome to the Official Page of Clemson iGEM!=
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[[File:TillmanPaw.png|850px|frameless|center]]
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==Our Mission==
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The FDA has maintained a zero-tolerance policy for several foodborne pathogens.  For example, a policy of “zero-tolerance” for ''Listeria monocytogenes'' in ready-to-eat foods means that the detection of any ''L. monocytogenes'' in either of two 25 gram samples of a food renders the food adulterated; the infectious dosage of ''E. coli'' O157:H7 has been determined to be 10 cells; the Environmental Protection Agency standard for ''E. coli'' O157:H7 in water is 40 cells per liter.  The current detection methods suffer from one or more of the following limitations: 1) the requirement of pre-enrichment and enrichment to increase the number of target pathogens, e.g., bio-chemical assays and immunoassays, 2) high detection limit, e.g., 10^3 – 10^5 CFU per ml or per gram of sample for immunoassays, 3) inability to distinguish viable from non-viable cells, e.g., PCR-based detection methods, 4) small sample volume capacity, e.g., microfluidic-based biosensors (µl instead of the required ml to liter capacity), 5) tedious detection procedures, and 6) the current high per-assay cost.
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The aim of this project is develop a Universal Self-Amplified (USA) Biosensor that addresses the aforementioned disadvantages of current detection methods. This two component system utilizes a universal signal amplification bacterial system and a unique pathogen-specific detection counterpart for a one-step detection of target microorganisms in a scalable volume.
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<image src="https://static.igem.org/mediawiki/2013/1/1e/ULeth2013_Sponsors_-_Silver.png"; width="200px"; height="100px" />&nbsp;&nbsp;&nbsp;&nbsp;
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<image src="https://static.igem.org/mediawiki/2013/4/4c/ULeth2013_Sponsors_-_Bronze.png"; width="300px"; height="100px" /></center></p>&nbsp;&nbsp;&nbsp;&nbsp;
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Revision as of 00:12, 17 October 2014


Project Overview

This year, the Lethbridge iGEM team is working to bring a whole new class of parts to the iGEM community: programmed ribosomal frameshifting elements. To do this, we have been working towards standardizing the PK401 pseudoknot for use within the BioBrick system. These RNA secondary structural elements cause the ribosome to switch between translational frames and give another degree of freedom when engineering genetic circuits.


WHAT?
  • Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community

WHY?
  • As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications

HOW?
  • We have brought pseudoknots to the iGEM community by:
    • Characterizing their function in a biobrick system
    • Designing software that enables others to dual code proteins
    • Ensuring that the release of these tools to the wider public does not pose a significant risk to the rest of the world

Sponsors


         

    
Retrieved from "http://2014.igem.org/Team:Clemson"