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Team:Cambridge-JIC/Guide/Transformation/Outline
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Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: | Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: | ||
The | The | ||
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-[[Team:Cambridge-JIC/Guide/Transformation/Cocultivation| cocultivation]] method | -[[Team:Cambridge-JIC/Guide/Transformation/Cocultivation| cocultivation]] method | ||
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-[[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| agar trap]] methods | -[[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| agar trap]] methods | ||
Revision as of 23:45, 16 October 2014
Outline
Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: The
- cocultivation method
- agar trap methods
Timing
The cocultivation method requires spores to be (at least) 5 days old prior to cocultivation with agrobacterium, and the agar trap 3 days old. Agrobacteria takes 3 days to grow after transformation.
For each method, your timing will be optimal if you prepare spores and transform agrobacteria on day 1.
