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Team:Cambridge-JIC/Guide/Constructs/Reporters
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These can be added instead of the stop codon for the reporters | These can be added instead of the stop codon for the reporters | ||
| - | These are clearly visible under | + | These are clearly visible under fluorescence/confocal microscopes. Sample images include: |
| - | LTI | + | LTI: |
| + | N7: | ||
[[File:CAM14_eGFP-N7.png|thumb|center|300px|Confocal Microscopy image of eGFP n7. Excitation:488, Detection range: 520-540.]] | [[File:CAM14_eGFP-N7.png|thumb|center|300px|Confocal Microscopy image of eGFP n7. Excitation:488, Detection range: 520-540.]] | ||
Revision as of 23:11, 16 October 2014
Contents |
Reporters
Fluorescent proteins
The following fluorescent proteins have been used with consistent results in Marchantia: [http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP.
Localisation Tags
A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: [http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI].
These can be added instead of the stop codon for the reporters
These are clearly visible under fluorescence/confocal microscopes. Sample images include:
LTI:
N7:
Fluorescent transformants are visible first from 3-5 days after transformation.
Other reporters
GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper]
