Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors

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=Agrobacterium Vectors=
=Agrobacterium Vectors=
==Ti Plasmids==
==Ti Plasmids==
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The Ti plasmid contains the genes required by Agrobacterium to transfect plants.
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Commonly used Ti plasmids are the pSoup plasmid. These are available from GenBank, or from any plant lab. Some of the Ti plasmids are commercial, so can't be submitted to the registry. Please contact a [mailto:salil92@gmail.com| team member] if you're having trouble sourcing the Ti plasmid.
Commonly used Ti plasmids are the pSoup plasmid. These are available from GenBank, or from any plant lab. Some of the Ti plasmids are commercial, so can't be submitted to the registry. Please contact a [mailto:salil92@gmail.com| team member] if you're having trouble sourcing the Ti plasmid.
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- T-DNA plasmid
 
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Level 1 + MoClo acceptors  are T-DNA plasmids. You can also consider pGreen2. If you are having trouble sourcing a plasmid suitable for transfection, please contact:
 
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Make sure your Ti plasmids and T-DNA plasmids have different antibiotic resistance markers, else you will not be able to select AT properly.
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== T-DNA plasmid==
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The T-DNA plasmid contains your DNA between the Left Border and Right Border of DNA transfer:
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Level 1 + [[Team:Cambridge-JIC/Guide/Constructs/MoCloAssembly|MoClo]] acceptors  are T-DNA plasmids. You can also consider pGreen2. If you are having trouble sourcing a plasmid suitable for transfection, consider contacting [http://www.tsl.ac.uk/staff/nicola-patron/|Nicola Patron, the author of the Modular Cloning Paper],
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When generating transformants, it is recommended to use the [Selection Cassette]
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Make sure your Ti plasmids and T-DNA plasmids have different antibiotic resistance markers, else you will not be able to select transformed Agrobacterium!

Revision as of 22:05, 16 October 2014

Agrobacterium Vectors

Ti Plasmids

The Ti plasmid contains the genes required by Agrobacterium to transfect plants.

Commonly used Ti plasmids are the pSoup plasmid. These are available from GenBank, or from any plant lab. Some of the Ti plasmids are commercial, so can't be submitted to the registry. Please contact a team member if you're having trouble sourcing the Ti plasmid.

T-DNA plasmid

The T-DNA plasmid contains your DNA between the Left Border and Right Border of DNA transfer:

Level 1 + MoClo acceptors are T-DNA plasmids. You can also consider pGreen2. If you are having trouble sourcing a plasmid suitable for transfection, consider contacting [http://www.tsl.ac.uk/staff/nicola-patron/|Nicola Patron, the author of the Modular Cloning Paper],

Make sure your Ti plasmids and T-DNA plasmids have different antibiotic resistance markers, else you will not be able to select transformed Agrobacterium!

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