Revision as of 23:15, 16 October 2014

Cooper Union 2014 iGEM

Hardware

OpenGelBox

OpenRoller

OpenFuge

Microfluidics

Microfluidics Platform

To optimize DNA synthesis using our De Novo Synthesis BioBricks, a microfluidics platform is being developed. It is unrealistic to have a lab worker pipetting for hours upon hours to produce large DNA sequences. This greatly aids in that by simplifying and automating the process.

This robotic arm is the first scaled down version of our automated system. This device was constructed in order to facilitate element timing using our in house TdT. The robotic arm, powered with Arduino, would lower the microfuge tube into the appropriate water baths for the experimental time periods. In house pumps, made from stepper motors, would pump in and out the correct solutions in the correct sequence. This device can deliver volumes as low as 10μls, making the robotic arm more a "macroscale" device.

Macro Arm Cad

Macro Pump Cad

These are the Cad files of the arm and pump being used in the platform.

Assembly

Fluidics Pump

Micro Sketch

Conclusion

In order to maximize the consumption of our in house TdT, a microfluidic device would be a more ideal platform for automation. The microfluidics platform has not yet been completed and is still in progress. We are hoping to prototype out a 10x scale version from a 3D print before pursuing soft lithography.

@@ Line 71: / Line 71: @@
 <h2>Microfluidics Platform</h2>
 <br>
+To optimize DNA synthesis using our De Novo Synthesis BioBricks, a microfluidics platform is being developed. It is unrealistic to have a lab worker pipetting for hours upon hours to produce large DNA sequences. This greatly aids in that by simplifying and automating the process.<br>
 <div class="center">
-<img src="https://static.igem.org/mediawiki/2014/8/83/CU_fluidics_Macro1.png" alt="Assembly1" style="height:400px">
+<img src="https://static.igem.org/mediawiki/2014/8/83/CU_fluidics_Macro1.png" alt="Assembly1" style="height:400px"><br><br>
+</div>
-</div><div class="left">
+This robotic arm is the first scaled down version of our automated system. This device was constructed in order to facilitate element timing using our in house TdT. The robotic arm, powered with Arduino, would lower the microfuge tube into the appropriate water baths for the experimental time periods. In house pumps, made from stepper motors, would pump in and out the correct solutions in the correct sequence. This device can deliver volumes as low as 10&mu;ls, making the robotic arm more a "macroscale" device.</div>
-<br>
-For use with the De Novo Synthesis portion of this project a microfluidics platform is being developed in conjunction. It is unrealistic to have a lab worker pipetting for hours upon hours to produce large DNA sequences. This greatly aids in that by simplifying and automating the process. The main components are made by laser cut acrylic, 3-D printed components and the pump and arm are controlled by an arduino. </div>
 <body>
@@ Line 98: / Line 99: @@
 <h2>Fluidics Pump</h2>
 <img src="https://static.igem.org/mediawiki/2014/9/9b/CU_fluidics_Pump1.png" alt="Fluidics Pump" style="width:304px;height:228px">
+<br><br>
 <h2>Micro Sketch</h2>
 <img src="https://static.igem.org/mediawiki/2014/4/44/CU_Micro_Sketch1.JPG" alt="Micro Sketch1" style="width:304px;height:228px">
@@ Line 106: / Line 107: @@
 <br>
-The microfluidics platform has not yet been completed and is still in progress.
+In order to maximize the consumption of our in house TdT, a microfluidic device would be a more ideal platform for automation. The microfluidics platform has not yet been completed and is still in progress. We are hoping to prototype out a 10x scale version from a 3D print before pursuing soft lithography.

Team:Cooper Union/Microfluidics

From 2014.igem.org