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Team:Groningen/Template/MODULE/Notebook/toolbox/week2
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Revision as of 20:17, 16 October 2014
14 - 20 Juli
A collection of constitutive promoters was also desirable for the toolbox.
We decided to use the CP promoter collection and test it in
Lactococcus lactis, that was BioBricked by
the Uppsala iGEM team of 2013. The CP promoters are registered as
BBa_K1033219 (CP1), BBa_K1033220 (CP8), BBa_K1033221 (CP11), BBa_K1033222 (CP29),
BBa_K1033223 (CP30), BBa_K1033224 (CP41) and BBa_K1033225 (CP44). These parts were
ordered from iGEM HQ, except for CP8 as for this promoter the sequencing
was inconsistent. The promoters were sent by iGEM HQ using
Escherichia coli containing pSB1C3 with the promoter as insert.
E. coli was grown and the plasmids with the promoter were
isolated.
