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Team:Freiburg/Content/Results/BioBrick
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<section> | <section> | ||
<h4>BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)</h4> | <h4>BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)</h4> | ||
| - | <p> <a href="http://parts.igem.org/Part:BBa_K1470004">Secreted Alkaline Phosphatase (SEAP)</a></p> | + | <p> <a href="http://parts.igem.org/Part:BBa_K1470004">Secreted Alkaline Phosphatase (SEAP)</a>is used in cell culture assays to quantify gene expression.</p> |
</section> | </section> | ||
<section> | <section> | ||
<h4>BBa_K1470005: engineered PDZ domain (ePDZ)</h4> | <h4>BBa_K1470005: engineered PDZ domain (ePDZ)</h4> | ||
| - | <p> <a href="http://parts.igem.org/Part:BBa_K1470005">engineered PDZ domain (ePDZ)</a></p> | + | <p> <a href="http://parts.igem.org/Part:BBa_K1470005">engineered PDZ domain (ePDZ)</a>is an important part of the blue light system.</p> |
</section> | </section> | ||
<section> | <section> | ||
<h4>BBa_K1470006: The Viral Vector (pMIG)</h4> | <h4>BBa_K1470006: The Viral Vector (pMIG)</h4> | ||
| - | <p> <a href="http://parts.igem.org/Part:BBa_K1470006">The Viral Vector (pMIG)</a></p> | + | <p> <a href="http://parts.igem.org/Part:BBa_K1470006">The Viral Vector (pMIG)</a>is the plasmid which is used to tranfect Phoenix cells with the gene to be packed into viral particles. It contains two LTRs and a multiple cloning site. </p> |
</section> | </section> | ||
<section> | <section> | ||
Revision as of 19:19, 16 October 2014
Bio Bricks
BBa_K1470000: mCat-1
mCat-1 is the receptor we introduced to human cells to be able to infect them using the MuLV
BBa_K1470001: Puromycin N-acetyl-transferase
Puromycin N-acetyl-transferase is an enzyme which we used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99% of uninfected cells will be dead within two days.
BBa_K1470002: Galactose-induced gene 4 (Gal4BD)
Galactose-induced gene 4 (Gal4BD) is used to investigate gene expression levels.
BBa_K1470003: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)
Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) is a regulatory RNA sequence, which promotes viral gene expression.
BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)
Secreted Alkaline Phosphatase (SEAP)is used in cell culture assays to quantify gene expression.
BBa_K1470005: engineered PDZ domain (ePDZ)
engineered PDZ domain (ePDZ)is an important part of the blue light system.
BBa_K1470006: The Viral Vector (pMIG)
The Viral Vector (pMIG)is the plasmid which is used to tranfect Phoenix cells with the gene to be packed into viral particles. It contains two LTRs and a multiple cloning site.
BBa_K1470007: Cas9-Nickase
Plasmids
mCat-1 Plasmids
Constitutive Expression
p14rz_003
- bla
- blaII
p14rz_003: pcDNA_SLC7A1_GFP
p14rz_004
- bla
- blaII
Freiburg2014_p14rz_004_pcDNA_SLC7A1_HA.png
p14rz_005
- bla
- blaII
Freiburg2014_p14rz_005_pcDNA_SLC7A1_mCherry.png
p14rz_006
- bla
- blaII
Freiburg2014_p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry.png
p14rz_007
- bla
- blaII
Freiburg2014_p14rz_007_pcDNA_SLC7A1_HA_mCherry.png
Red Light induced
p14rz_010
Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png
p14rz_011
Freiburg2014_p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry.png
Blue Light induced
p14rz_008
Freiburg2014_p14rz_008_Gal4_SLC7A1.png
p14rz_009
Freiburg2014_p14rz_009_Gal4_SLC7A1_HA.png
Light System
Red Light
Blue Light
UV Light
p14ls_001
p14ls_001_COP1-VP16_IRES_TetR-UVR8
p14ls_002
Freiburg2014_p14ls_002_Gal4_mCherry
