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Team:Arizona State/project
From 2014.igem.org
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<p>Biodesiel production | <p>Biodesiel production | ||
| - | Current system: When attempting to produce biodesiel in E. Coli, certain problems emerge, especially with the intermediate products. E. coli has to use pyruvate created in glycolysis to produce both intermediates of the fatty acid ethyl ester: ethanol and acyl-COAs. | + | Current system: When attempting to produce biodesiel in E. Coli, certain problems emerge, especially with the intermediate products. E. coli has to use pyruvate created in glycolysis to produce both intermediates of the final biodesiel final product, fatty acid ethyl ester: ethanol and acyl-COAs. The solution to this is to get two colonies of E. Coli to create the intermediates individually. This way, there will be no competition for use of resources inside of the individual cells. With the two colonies growing together, a greater titer of the final product (FAEE's) can be accomplished. |
| - | <img src="https://2014.igem.org/File:Metabolic_pathway.jpg"> | + | |
| - | + | <p><u>Engineered Pathway:</u> | |
| + | </p> | ||
| + | The first intermediate, ethanol, is formed using a two enzyme plasmid. Pdc and adhB take pyruvate from glycolisis and convert it into first, acetaldehyde, and then into ethanol. | ||
| + | <p> | ||
| + | The second intermediates, acyl coa's, are formed with a thioesterase combined with the four components of the Acc plasmid (A,B,C,D). The two intermediates are combined with a wax-synthase and a coa-ligase to produce fatty acid ethyl esters. | ||
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| + | <tr> <td colspan="3" height="100px"><img src="https://2014.igem.org/File:Metabolic_pathway.jpg" width="975" height="77" align="center"/></td></tr> | ||
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</p> | </p> | ||
<p> </p></td> | <p> </p></td> | ||
Revision as of 19:34, 16 October 2014
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Fabrication of Biofuels with a multi-strain system
Biodesiel production Current system: When attempting to produce biodesiel in E. Coli, certain problems emerge, especially with the intermediate products. E. coli has to use pyruvate created in glycolysis to produce both intermediates of the final biodesiel final product, fatty acid ethyl ester: ethanol and acyl-COAs. The solution to this is to get two colonies of E. Coli to create the intermediates individually. This way, there will be no competition for use of resources inside of the individual cells. With the two colonies growing together, a greater titer of the final product (FAEE's) can be accomplished. Engineered Pathway: The first intermediate, ethanol, is formed using a two enzyme plasmid. Pdc and adhB take pyruvate from glycolisis and convert it into first, acetaldehyde, and then into ethanol.The second intermediates, acyl coa's, are formed with a thioesterase combined with the four components of the Acc plasmid (A,B,C,D). The two intermediates are combined with a wax-synthase and a coa-ligase to produce fatty acid ethyl esters. | ||||||||||
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