Team:The Tech Museum/Notebook

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Revision as of 18:42, 16 October 2014

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Notebook

Timeline of major events:
May: Basic concept research and design
June: Software development initiated; plasmid design started and promoter-RBS’s picked
July: Wetlab testing of possible color protein combinations
August 10: Design of complete tri-color plasmids finalized
August 24: Assembly of tri-color plasmid pools completed (DNA2.0)
September 10: Tri-color plasmids optimized on museum wetlab setup
September 29: Software fully integrated with hardware on mobile exhibit
September 30 - October 3: Prototyping of mobile exhibit with museum staff and visitors
October 6 - October 17: Data collection on the museum floor with visitor!

Biology Details

Design of tri-color plasmid pool (June 2014)
9 promoter-rbs pairs of varying strength from Kosuri et al paper (2013):

	Promoter - RBS Pairs	Sequence
1	BBa_J23117 - BBa_J61112	TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCCAATCTCTAGAGAAAGAGGTGACATAC
2	BBa_J23104 - BBa_J61107	TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCTTACGTCTAGAGAAAGAAGAGACTCAC
3	apFAB78 - apFAB954	TTGACATTTATCCCTTGCGGCGATAGATTTAACGTATGACGGATCTTAATCTAGCTCAGGACAATTT
4	apFAB76 - apFAB927	TTGACATTTATCCCTTGCGGCGATATAATAGATATCTTAATCTAGCCCGGGAGTTTTTTCATTCCGGATCTTAATCTAGCTGGGGACTGTTT
5	apFAB70 - apFAB844	TTGACATCGCATCTTTTTGTACCTATAATGTGTGGATAGAGTATCTTAATCTAGCAGGGGACACTTT
6	BBa_J23104 - apFAB909	TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCTTACGATCTTAATCTAGCGAAGGATAGTTT
7	apFAB45 - apFAB901	AAAAAGAGTATTGACTTCGCATCTTTTTGTACCTATAATGTGTGGATAGCGG
8	apFAB92 - apFAB863	AAAAAATTTATTTGCTTTCAGGAAAATTTTTCTGCATAATTATTTCATGGAGCATCTTAATCTAGCGGGGGAGCGTTT
9	apFAB71 - salis-4-10	TTGACATCGCATCTTTTTGTACCTATAATAGATTCATGATGAAATCTCTTTTATCAAATATAAGCAGGAT

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 <td>Promoter - RBS Pairs</td>
 <td>Sequence</td>