Team:StanfordBrownSpelman/Cellulose Cross Linker

From 2014.igem.org

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   The goal of this subproject is to create a cellulose cross-linking protein to increase material strength and allow for the modular attachment of biological sensors.  This fusion protein contains two distinct cellulose-binding domains on either side of a streptavidin domain. The cellulose-binding domains cross link the cellulose fibers while the streptavidin serves as a binding domain for biological sensors. The interaction between SA (streptavidin) and biotin is one of the strongest non-covalent interactions in Nature. Therefore a cell expressing an outer membrane protein that has been biotinylated will bind tightly to this domain. This will allow our UAV to make use of a number of biological sensors.
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   The goal of this subproject is to create a cellulose cross-linking protein to increase material strength and allow for the modular attachment of biological sensors.  This fusion protein contains two distinct cellulose-binding domains on either side of a streptavidin domain. The cellulose-binding domains cross link the cellulose fibers while the streptavidin serves as a binding domain for biological sensors. The interaction between SA (streptavidin) and biotin is one of the strongest non-covalent interactions in nature [1]. Therefore a cell expressing an outer membrane protein that has been biotinylated will bind tightly to this domain. This will allow our UAV to make use of a number of biological sensors.
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   <h5><center>References</h5>
   <h5><center>References</h5>
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  1. Chivers, Claire <i>et al.</i> (2011) How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer. <i>Biochem. J.</i> 55. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/21241253">24136966</a>.<br>
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  <div class="sub5"><a href="work/PUT-PDF-REFERENCE-HEREpdf">● Have a link or reference? Put it here!</a></div>
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  <div class="sub5"><a href="work/PUT-PDF-REFERENCE-HEREpdf">● Link, acknowledgement, or reference 2</a></div>
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  <div class="sub5"><a href="work/PUT-PDF-REFERENCE-HEREpdf">● Additional links, acknowledgements, and references</a></div>
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Revision as of 18:52, 16 October 2014

Stanford–Brown–Spelman iGEM 2014 — Cellulose Acetate

Methods
Methods here.


Figure 1. Figure caption here.
Results
Our initial approach was to include two identical cellulose-binding domains on either side of the streptavidin domain. However, this led to numerous problems with molecular cloning due to the repetitive nature of the sequence. We changed our approach to using two cellulose-binding domains with different sequences. This allowed us to successfully conduct the molecular cloning.
References
1. Chivers, Claire et al. (2011) How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer. Biochem. J. 55. PMID: 24136966.
Additional Information
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