Team:Hong Kong-CUHK/project-5.html

From 2014.igem.org

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        <h2>Future Application</h2>
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                    <strong><u>Control enzymatic reactions</u>
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                    <li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li>
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                    <li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li>
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                    <strong><u>Bacterial Screening</u>
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                <ul>
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                    <li>Utilize the BiFC</li>
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                    <li>Load cells onto multi-well plates</li>
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                    <li>Each well serves as a Pixel</li>
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                    <img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1">
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                    <strong><u>Degradation of cellular proteins</u>
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                    <li>Utilize the Ubiquitin/26S proteasome system</li>
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                    <li>Linking the voltage switch with E1/E2/E3 protein</li>
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                    <li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li>
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                <p></p>
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                    <strong><u>Study genes using protein complementation</u>
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                    <li>Split genes into two parts, and insert into our system</li>
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                    <li>Use voltage switch to control the split and refold of a gene.</li>
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                    <li>Possible tool for the screening of new protein complementation compatible proteins.</li>
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                    <strong><u>Study Membrane Potential of Organelles</u>
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                    <li>Target voltage switch system with BiFC onto the membrane of organelles interested</li>
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                    <li>Obtain fluorescent signal</li>
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                    <li>Estimate membrane potential</li>
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Revision as of 20:49, 18 November 2014

We are now updating the page! Please come later Thank you!