Team:Warwick/Parts

From 2014.igem.org

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     <h2>5' Promoter</h2>
     <h2>5' Promoter</h2>
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     <p>This is derived from the 5i UTR of the HCV virus strain 1b isolate Con1. The secondary structure of this sequence acts as a binding site and initiates replication of the system by RdRp. It also includes the first 16 amino acids of the first gene of HCV as this has been shown to increase the efficacy of binding of the RdRp to the 5' promoter.</p>
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Revision as of 21:02, 16 October 2014

5' Promoter

This is derived from the 5i UTR of the HCV virus strain 1b isolate Con1. The secondary structure of this sequence acts as a binding site and initiates replication of the system by RdRp. It also includes the first 16 amino acids of the first gene of HCV as this has been shown to increase the efficacy of binding of the RdRp to the 5' promoter.

IRES

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MS2 Box

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Neomycin Resistance

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Aptazyme

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siRNA

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MS2 Coat Protein

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P2A

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RdRp

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3' Promoter

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