"
Team:UFAM Brazil/Protocol1
From 2014.igem.org
Manickchand (Talk | contribs) |
Manickchand (Talk | contribs) |
||
| Line 43: | Line 43: | ||
<p>After DNA cloning resuspended from parts kit by strains of E. coli RR1, DH5α and DH10B, we used this strain of E. coli to demethylate the XbaI restriction site, enabling our genetic constructs to be efficient, taking into account that the same dam is negative. </p> | <p>After DNA cloning resuspended from parts kit by strains of E. coli RR1, DH5α and DH10B, we used this strain of E. coli to demethylate the XbaI restriction site, enabling our genetic constructs to be efficient, taking into account that the same dam is negative. </p> | ||
| - | <p style="margin-left:50px">• <i> | + | <p style="margin-left:50px">• <i>Escherichia coli</i>JM110</p> |
<p></p> | <p></p> | ||
Revision as of 15:54, 16 October 2014
Cell lines | ||
|
Bacteria used, their genotypes and functions for our Project. • Escherichia coli DH5α Genotype: F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– Nalidixic acid resistant. We used this strain of E. coli for DNA cloning resuspended from parts kit. In addition to this role, it was chosen for our tests with mercury. • Escherichia coli DH10B Genotype: F- endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ- Antibiotic Resistance: streptomycin. We used this strain of E. coli for DNA cloning resuspended from parts kit Escherichia coli RR1 Genotype: F- mcrB mrr hsdS20(rB- mB-) recA+ leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- Antibiotic Resistance: streptomycin. We used this strain of E. coli for DNA cloning resuspended from parts kit Escherichia coli RR1 • Escherichia coli ER2925 Genotype: ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 Antibiotic Resistance: kanamycin, streptomycin. After DNA cloning resuspended from parts kit by strains of E. coli RR1, DH5α and DH10B, we used this strain of E. coli to demethylate the XbaI restriction site, enabling our genetic constructs to be efficient, taking into account that the same dam is negative. • Escherichia coliJM110 | ||
