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Team:Groningen/Template/MODULE/PP/collaborations/methods
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| + | Used strains B. subtilus: | ||
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| + | <div class="item">1. ATCC6633 LB</div> | ||
| + | <div class="item">2. ATCC6633 SMM (Preparation of SMM (Spizizen, 1958) with trace elements (Harwood and Archibald, 1990)</div> | ||
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Revision as of 14:45, 16 October 2014
Methods
We I performed a halo experiment to test if S. aureus has sensitivity to subtilin, produced by B. subtilis. This was performed in the ML-II lab of the MolGen group at the University of Groningen and supervised by Renske van Raaphorst. Below we report what steps are taken and give an overview of the results. Also we provide you with some references of the strains we used.
30-09-2014
- Inoculated three different strains of S. aureus (see below) in 3 ml Nutrient Broth (Difco). Also two different B. subtilis strains were inoculated under different conditions (see below). The ATCC6633 strain was inoculated in 3 ml LB and in 3 ml of the defined medium SMM. Also the 168 strain was inoculated in 3 ml LB. These samples were grown overnight at 37ºC in a shaking incubator.
- Inoculated three different strains of S. aureus (see below) in 3 ml Nutrient Broth (Difco). Also two different B. subtilis strains were inoculated under different conditions (see below). The ATCC6633 strain was inoculated in 3 ml LB and in 3 ml of the defined medium SMM. Also the 168 strain was inoculated in 3 ml LB. These samples were grown overnight at 37ºC in a shaking incubator.
Used strains S. aureus: (All strains were provided by Auke van Heel)
1. CAL (MRSA obtained at the UMCG hospital)
2. NWZ (MRSA obtained at the UMCG hospital)
3. CECT240 (Staphylococcus aureus subsp. aureus, Rosenbach 1884)
Used strains B. subtilus:
1. ATCC6633 LB
2. ATCC6633 SMM (Preparation of SMM (Spizizen, 1958) with trace elements (Harwood and Archibald, 1990)
3. 168 LB
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