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Team:ZJU-China/Tools
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Revision as of 12:46, 16 October 2014
| Team Sponsor | Contact us | ||
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Team Forum(BBS): | ZJU-China 2014 Team Forum on www.zjubiolab.zju.edu.cn | |
| Post Address: | Biolab Center Room 413, ZJU Zijin'gang Campus, Yuhang Tang Road No.866, Hangzhou, Zhejiang |
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| Powered by Media Wiki. Zhejiang University. | Email Address: | zjuchina2014 @ 163.com | |
GS-Box
An useful bioinformatics tool for Gene-Socket
Every excellent technique should be simple and efficient to use! As an excellent technique, gene-socket also does well in this respect------ it has its' own optimization tool: GS-BOX. GS-BOX is a bioinformatics tool which is designed for customs to build their own gen-sockets easily and efficiently. What need the customs do with the tool? Just input the gene circuit sequences and then GS-BOX will design the experimental process automatically and every detail that customs will need to use!
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Service
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Design Principle
Homeodomains:
1.Don't have similar sequences with E.coli (to avoid off-target effects)
2.Don't have similar sequences with custom's parts (to avoid off-target effects)
3.Fit the subsequent steps' requiring
Primer:
1.GC content & TM value
2.Duplex formation and hairpin
3.proper length -
Design Method
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Mauris sed mauris. Praesent imperdiet, nunc ut sollicitudin hendrerit, nisi tellus mollis leo, blandit pharetra nulla orci id eros. Nullam ut nunc. Praesent lacus lacus, tempor a, dignissim eu, tristique ac, tortor. Sed faucibus. Integer eleifend lacus ac neque. Fusce tempus. In hac habitasse platea dictumst. Nulla arcu neque, gravida at, rhoncus id, pharetra vitae, tortor. Aenean vestibulum consequat augue. -
How to use
If you just want to build a normal gene circuit, input your mount of parts and sequences; If your circuit contains some particular parts(for example, an terminator primer or want to make your parts seamless connection),please choose the "special mode" according to your situation.
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Contact
If you have any questions when using GS-Box, please contact us.
