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Team:UST Beijing/Parts
From 2014.igem.org
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<img align="center" src="https://static.igem.org/mediawiki/2014/1/11/Parts1.jpg"></a> | <img align="center" src="https://static.igem.org/mediawiki/2014/1/11/Parts1.jpg"></a> | ||
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| - | <p>PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.</p></div> | + | <p align="left">PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.</p></div> |
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Revision as of 11:22, 16 October 2014
Parts We Used
Mitochondrial proteorhodopsin (humanized genetic code bias)
PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.
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