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Team:NEFU China/Recycling
From 2014.igem.org
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<p>In order to verify the synthesis of the CdS nanocrystal in <em>E.coli</em> (<em>Rosetta plysS</em>), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.</p> | <p>In order to verify the synthesis of the CdS nanocrystal in <em>E.coli</em> (<em>Rosetta plysS</em>), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.</p> | ||
| - | <p>Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at | + | <p>Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 80-keV electron energy.</p> |
<p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/9/9c/NEFU_China_labnote_fig42.png" class="img-thumbnail"></p> | <p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2014/9/9c/NEFU_China_labnote_fig42.png" class="img-thumbnail"></p> | ||
<h2 class="subtitle">Reference.</h2> | <h2 class="subtitle">Reference.</h2> | ||
Revision as of 09:23, 16 October 2014
Recycling
TEM Protocol.
In order to verify the synthesis of the CdS nanocrystal in E.coli (Rosetta plysS), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.
Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 80-keV electron energy.
Reference.
- Mareike Hartmann,Marina Berditsch,Jacques Hawecker,Mohammad Fotouhi Ardakani,Dagmar Gerthsen,Anne S. Ulrich1.Damage of the Bacterial Cell Envelope by Antimicrobial Peptides Gramicidin S and PGLa as Revealed by Transmission and Scanning Electron Microscopy Antimicrob Agents Chemother. 2010; 54(8): 3132–3142.
- Congcong Mi,Yanyan Wang,Jingpu Zhang,Huaiqing Huang,Linru Xu,Shuo Wang,Xuexun Fang,Jin Fang,Chuanbin Mao,Shukun Xu.Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli. Journal of biotechnology 153(2011) 125-132
- Ivan Sondi,Branka Salopek-Sondi.Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182
